Home Newsletters Pulmonary Cell News In Well-Differentiated Primary Human Bronchial Epithelial Cells, TGF-β1 and TGF-β2 Induce Expression...

In Well-Differentiated Primary Human Bronchial Epithelial Cells, TGF-β1 and TGF-β2 Induce Expression of Furin

0
Investigators assessed the suitability of primary human bronchial epithelial cells maintained in air-liquid interface as an experimental system for studying SARS-CoV-2 infection in vitro.
[American Journal of Physiology-Lung Cellular and Molecular Physiology]
1254621 ZH77BHVV items 1 apa 0 default asc 1 156162 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-3852e05baa2b57bb96e56b70c3c8e8a3%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22ZH77BHVV%22%2C%22library%22%3A%7B%22id%22%3A1254621%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22O%27Sullivan%20et%20al.%22%2C%22parsedDate%22%3A%222020-11-11%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EO%26%23x2019%3BSullivan%2C%20M.%20J.%2C%20Mitchel%2C%20J.%20A.%2C%20Mwase%2C%20C.%2C%20McGill%2C%20M.%2C%20Kanki%2C%20P.%2C%20%26amp%3B%20Park%2C%20J.-A.%20%282020%29.%20In%20well-differentiated%20primary%20human%20bronchial%20epithelial%20cells%2C%20TGF-%26%23x3B2%3B1%20and%20TGF-%26%23x3B2%3B2%20induce%20expression%20of%20furin.%20%3Ci%3EAmerican%20Journal%20of%20Physiology-Lung%20Cellular%20and%20Molecular%20Physiology%3C%5C%2Fi%3E.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1152%5C%2Fajplung.00423.2020%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1152%5C%2Fajplung.00423.2020%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D1254621%26amp%3Bitem_key%3DZH77BHVV%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22In%20well-differentiated%20primary%20human%20bronchial%20epithelial%20cells%2C%20TGF-%5Cu03b21%20and%20TGF-%5Cu03b22%20induce%20expression%20of%20furin%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michael%20J%22%2C%22lastName%22%3A%22O%27Sullivan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jennifer%20A%22%2C%22lastName%22%3A%22Mitchel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Chimwemwe%22%2C%22lastName%22%3A%22Mwase%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maureen%22%2C%22lastName%22%3A%22McGill%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Phyllis%22%2C%22lastName%22%3A%22Kanki%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jin-Ah%22%2C%22lastName%22%3A%22Park%22%7D%5D%2C%22abstractNote%22%3A%22Background%3A%20The%20COVID-19%20pandemic%20is%20an%20ongoing%20threat%20to%20public%20health.%20Since%20the%20identification%20of%20COVID-19%2C%20the%20disease%20caused%20by%20SARS-CoV-2%2C%20no%20drugs%20have%20been%20developed%20to%20specifically%20target%20SARS-CoV-2.%20To%20develop%20effective%20and%20safe%20treatment%20options%2C%20a%20better%20understanding%20of%20cellular%20mechanisms%20underlying%20SARS-CoV-2%20infection%20is%20required.%20To%20fill%20this%20knowledge%20gap%2C%20researchers%20require%20reliable%20experimental%20systems%20that%20express%20the%20host%20proteins%20necessary%20for%20the%20cellular%20entry%20of%20SARS-CoV-2.%20These%20proteins%20include%20the%20viral%20receptor%2C%20ACE2%20and%20the%20proteases%20TMPRSS2%20and%20furin.%20A%20number%20of%20studies%20have%20reported%20cell-type%20specific%20expression%20of%20the%20genes%20encoding%20these%20molecules.%20However%2C%20less%20is%20known%20about%20the%20protein%20expression%20of%20these%20molecules.%20Methods%3A%20We%20assessed%20the%20suitability%20of%20primary%20human%20bronchial%20epithelial%20%28HBE%29%20cells%20maintained%20in%20air-liquid%20interface%20%28ALI%29%20as%20an%20experimental%20system%20for%20studying%20SARS-CoV-2%20infection%20in%20vitro.%20During%20cellular%20differentiation%2C%20we%20measured%20the%20expression%20of%20ACE2%2C%20TMPRSS2%2C%20and%20furin%20over%20progressive%20ALI%20days%20by%20RT-qPCR%2C%20western%20blot%20and%20immunofluorescence%20staining.%20We%20also%20explored%20the%20effect%20of%20the%20fibrotic%20cytokine%20TGF-b%20on%20the%20expression%20of%20these%20proteins%20in%20well-differentiated%20HBE%20cells.%20Results%5C%2FDiscussion%3A%20Like%20ACE2%2C%20TMPRSS2%20and%20furin%20proteins%20are%20localized%20in%20differentiated%20ciliated%20cells%20as%20confirmed%20by%20immunofluorescence%20staining.%20These%20data%20suggest%20that%20well-differentiated%20HBE%20cells%20maintained%20in%20air-liquid%20interface%20is%20a%20reliable%20in%20vitro%20system%20for%20investigating%20cellular%20mechanisms%20of%20SARS-CoV-2%20infection.%20We%20further%20identified%20that%20profibrotic%20mediators%2C%20TGF-%5Cu03b21%20and%20TGF-%5Cu03b22%2C%20increase%20the%20expression%20of%20furin%2C%20which%20is%20a%20protease%20required%20for%20the%20cellular%20entry%20of%20SARS-CoV-2.%22%2C%22date%22%3A%22November%2011%2C%202020%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1152%5C%2Fajplung.00423.2020%22%2C%22ISSN%22%3A%221040-0605%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fjournals.physiology.org%5C%2Fdoi%5C%2Fabs%5C%2F10.1152%5C%2Fajplung.00423.2020%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222020-11-12T22%3A03%3A43Z%22%7D%7D%5D%7D
O’Sullivan, M. J., Mitchel, J. A., Mwase, C., McGill, M., Kanki, P., & Park, J.-A. (2020). In well-differentiated primary human bronchial epithelial cells, TGF-β1 and TGF-β2 induce expression of furin. American Journal of Physiology-Lung Cellular and Molecular Physiology. https://doi.org/10.1152/ajplung.00423.2020 Cite
Abstract
Exit mobile version