The authors describe a platform to cultivate muAstV using air–liquid interface cultures derived from mouse enteroids, which support apical infection and release.
[Mucosal Immunology]
6445218
{6445218:LKRIK2XV}
apa
50
1
159818
https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-9e580e7ded2d2478347648a4462982b3%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22LKRIK2XV%22%2C%22library%22%3A%7B%22id%22%3A6445218%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Ingle%20et%20al.%22%2C%22parsedDate%22%3A%222021-03-05%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EIngle%2C%20H.%2C%20Hassan%2C%20E.%2C%20Gawron%2C%20J.%2C%20Mihi%2C%20B.%2C%20Li%2C%20Y.%2C%20Kennedy%2C%20E.%20A.%2C%20Kalugotla%2C%20G.%2C%20Makimaa%2C%20H.%2C%20Lee%2C%20S.%2C%20Desai%2C%20P.%2C%20McDonald%2C%20K.%20G.%2C%20Diamond%2C%20M.%20S.%2C%20Newberry%2C%20R.%20D.%2C%20Good%2C%20M.%2C%20%26amp%3B%20Baldridge%2C%20M.%20T.%20%282021%29.%20Murine%20astrovirus%20tropism%20for%20goblet%20cells%20and%20enterocytes%20facilitates%20an%20IFN-%26%23x3BB%3B%20response%20in%20vivo%20and%20in%20enteroid%20cultures.%20%3Ci%3EMucosal%20Immunology%3C%5C%2Fi%3E%2C%201%26%23x2013%3B11.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41385-021-00387-6%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41385-021-00387-6%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6445218%26amp%3Bitem_key%3DLKRIK2XV%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Murine%20astrovirus%20tropism%20for%20goblet%20cells%20and%20enterocytes%20facilitates%20an%20IFN-%5Cu03bb%20response%20in%20vivo%20and%20in%20enteroid%20cultures%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Harshad%22%2C%22lastName%22%3A%22Ingle%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ebrahim%22%2C%22lastName%22%3A%22Hassan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jana%22%2C%22lastName%22%3A%22Gawron%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Belgacem%22%2C%22lastName%22%3A%22Mihi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yuhao%22%2C%22lastName%22%3A%22Li%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elizabeth%20A.%22%2C%22lastName%22%3A%22Kennedy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gowri%22%2C%22lastName%22%3A%22Kalugotla%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Heyde%22%2C%22lastName%22%3A%22Makimaa%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sanghyun%22%2C%22lastName%22%3A%22Lee%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pritesh%22%2C%22lastName%22%3A%22Desai%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Keely%20G.%22%2C%22lastName%22%3A%22McDonald%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michael%20S.%22%2C%22lastName%22%3A%22Diamond%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rodney%20D.%22%2C%22lastName%22%3A%22Newberry%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Misty%22%2C%22lastName%22%3A%22Good%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Megan%20T.%22%2C%22lastName%22%3A%22Baldridge%22%7D%5D%2C%22abstractNote%22%3A%22Although%20they%20globally%20cause%20viral%20gastroenteritis%20in%20children%2C%20astroviruses%20are%20understudied%20due%20to%20the%20lack%20of%20well-defined%20animal%20models.%20While%20murine%20astroviruses%20%28muAstVs%29%20chronically%20infect%20immunodeficient%20mice%2C%20a%20culture%20system%20and%20understanding%20of%20their%20pathogenesis%20is%20lacking.%20Here%2C%20we%20describe%20a%20platform%20to%20cultivate%20muAstV%20using%20air%5Cu2013liquid%20interface%20%28ALI%29%20cultures%20derived%20from%20mouse%20enteroids%2C%20which%20support%20apical%20infection%20and%20release.%20Chronic%20muAstV%20infection%20occurs%20predominantly%20in%20the%20small%20intestine%20and%20correlates%20with%20higher%20interferon-lambda%20%28IFN-%5Cu03bb%29%20expression.%20MuAstV%20stimulates%20IFN-%5Cu03bb%20production%20in%20ALI%2C%20recapitulating%20our%20in%20vivo%20findings.%20We%20demonstrate%20that%20goblet%20cells%20and%20enterocytes%20are%20targets%20for%20chronic%20muAstV%20infection%20in%20vivo%2C%20and%20that%20infection%20is%20enhanced%20by%20parasite%20co-infection%20or%20type%202%20cytokine%20signaling.%20Depletion%20of%20goblet%20cells%20from%20ALI%20limits%20muAstV%20infection%20in%20vitro.%20During%20chronic%20infection%2C%20muAstV%20stimulates%20IFN-%5Cu03bb%20production%20in%20infected%20cells%20and%20induces%20ISGs%20throughout%20the%20intestinal%20epithelium%20in%20an%20IFN-%5Cu03bb-receptor-dependent%20manner.%20Collectively%2C%20our%20study%20provides%20insights%20into%20the%20cellular%20tropism%20and%20innate%20immune%20responses%20to%20muAstV%20and%20establishes%20an%20enteroid-based%20culture%20system%20to%20propagate%20muAstV%20in%20vitro.%22%2C%22date%22%3A%222021-03-05%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41385-021-00387-6%22%2C%22ISSN%22%3A%221935-3456%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41385-021-00387-6%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-03-05T23%3A45%3A12Z%22%7D%7D%5D%7D
Ingle, H., Hassan, E., Gawron, J., Mihi, B., Li, Y., Kennedy, E. A., Kalugotla, G., Makimaa, H., Lee, S., Desai, P., McDonald, K. G., Diamond, M. S., Newberry, R. D., Good, M., & Baldridge, M. T. (2021). Murine astrovirus tropism for goblet cells and enterocytes facilitates an IFN-λ response in vivo and in enteroid cultures. Mucosal Immunology, 1–11. https://doi.org/10.1038/s41385-021-00387-6 Cite
