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Replication Is the Key Barrier during the Dual-Host Adaptation of Mosquito-Borne Flaviviruses

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Using the pseudoinfectious reporter virus particle and reverse genetics systems, researchers found dual-host affiliated insect-specific flaviviruses entered vertebrate cells as efficiently as the mosquito-borne flaviviruses but failed to initiate replication.
[Proceedings of the National Academy of Sciences of the United States of America]

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