Scientists constructed minigene circuits based on the CRISPReader, a technology used to control promoter-less gene expression in a robust manner.
The authors focus on the recent findings concerning the roles of immune checkpoint molecules and receptors in the regulation of NK cell function, as well as their potential application in tumor immunotherapy.
[Signal Transduction and Targeted Therapy]
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Scientists provide an overview of the current clinical status of CAR-T and oncolytic virus (OV) therapies. While preclinical studies have demonstrated curative potential, the benefit of CAR-T and OVs as single agent treatments remains limited to a subset of patients.
Scientists demonstrated significant differences in the ratio of CD4/CD8 T cells and the number of CD56+ NKG2D+ NK cells and γδ T cells between ovarian endometrioma patients and control subjects.
Researchers conducted a population-based case–control study investigating the association between chronic rhinosinusitis and premorbid autoimmune diseases by using the National Health Insurance Research Database in Taiwan.
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Shih, L.-C., Hsieh, H.-H., Tsay, G. J., Lee, I. T., Tsou, Y.-A., Lin, C.-L., Shen, T.-C., Bau, D.-T., Tai, C.-J., Lin, C.-D., & Tsai, M.-H. (2020). Chronic rhinosinusitis and premorbid autoimmune diseases: a population-based case–control study. Scientific Reports, 10(1), 18635. https://doi.org/10.1038/s41598-020-75815-x Cite
Investigators analyzed peripheral-blood exome sequence data independent of clinical phenotype and inheritance pattern to identify deleterious mutations in ubiquitin-related genes.
[New England Journal of Medicine]
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Beck, D. B., Ferrada, M. A., Sikora, K. A., Ombrello, A. K., Collins, J. C., Pei, W., Balanda, N., Ross, D. L., Cardona, D. O., Wu, Z., Patel, B., Manthiram, K., Groarke, E. M., Gutierrez-Rodrigues, F., Hoffmann, P., Rosenzweig, S., Nakabo, S., Dillon, L. W., Hourigan, C. S., … Grayson, P. C. (2020). Somatic Mutations in UBA1 and Severe Adult-Onset Autoinflammatory Disease. New England Journal of Medicine. https://doi.org/10.1056/NEJMoa2026834 Cite
Scientists characterized the phenotype of the peripheral blood circulating cytotoxic cells of 30 healthy donors, in particular the repertoire of cytotoxic markers, using flow cytometry. In parallel, they characterized the antibody-dependent cellular cytotoxicity effector functions of these primary cells by measuring their cytolytic activity against a cancer cell-line expressing HER2 in the presence of trastuzumab and with regards to FCGR3A genotype.
[Frontiers in Immunology]
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Wieckowski, S., Avenal, C., Orjalo, A. V. J., Gygax, D., & Cymer, F. (2020). Toward a Better Understanding of Bioassays for the Development of Biopharmaceuticals by Exploring the Structure-Antibody-Dependent Cellular Cytotoxicity Relationship in Human Primary Cells. Frontiers in Immunology, 11. https://doi.org/10.3389/fimmu.2020.552596 Cite
Researchers hypothesized that head and neck squamous cell carcinoma recurrent tumors successfully evade anti-tumor immune response and aimed to reveal tumor immune microenvironment changes of primary tumors.
[Clinical Cancer Research]
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Watermann, C., Pasternack, H., Idel, C., Ribbat-Idel, J., Brägelmann, J., Kuppler, P., Offermann, A., Jonigk, D., Kühnel, M., Schröck, A., Dreyer, E., Rosero, C., Nathansen, J., Dubrovska, A., Tharun, L., Kirfel, J., Wollenberg, B., Perner, S., & Krupar, R. (2020). Recurrent HNSCC harbor an immunosuppressive tumor immune microenvironment suggesting successful tumor immune evasion. Clinical Cancer Research. https://doi.org/10.1158/1078-0432.CCR-20-0197 Cite
Scientists combined lineage tracing with antibody cloning from single B cells to examine the role of affinity in B cell selection into germinal centers and the memory B cell compartment in mice immunized with an HIV-1 antigen.
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Scientists screened the ability of multiple adeno-associated virus serotypes to transduce lung bud organoids, a model of human lung parenchyma generated from human embryonic stem cells.
[Stem Cell Research & Therapy]
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To facilitate pathogenesis studies in vivo, scientists developed an inducible CRISPR interference system that enabled genome-wide fitness testing in one sequencing step
[Cell Host & Microbe]
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