The Solupore system was used to deliver mRNA and CRISPR/Cas9 guide RNA ribonucleoprotein cargos to T cells, and efficiency was measured by flow cytometry.
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Kavanagh, H., Dunne, S., Martin, D. S., McFadden, E., Gallagher, L., Schwaber, J., Leonard, S., & O’Dea, S. (2021). A novel non-viral delivery method that enables efficient engineering of primary human T cells for ex vivo cell therapy applications. Cytotherapy, 0(0). https://doi.org/10.1016/j.jcyt.2021.03.002 Cite
The authors observed a high correlation in LILRB4 expression with other immune inhibitory receptors. After tumor challenge, LILRB4−/− mice and mice treated with anti-LILRB4 antibody showed reduced tumor burden and increased survival.
[Journal of Experimental Medicine]
High levels of FOXP3, CTLA4 and CD25 expression, demethylation of the FOXP3 promoter and high suppressive ability were found with no differences between Tregs and RA+Tregs.
[European Journal of Immunology]
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Romano, M., Sen, M., Scottà, C., Alhabbab, R. Y., Rico‐Armada, A., Lechler, R. I., Burch, M., & Lombardi, G. (n.d.). Isolation and expansion of thymus-derived regulatory T cells for use in paediatric heart transplant patients. European Journal of Immunology, n/a(n/a). https://doi.org/https://doi.org/10.1002/eji.202048949 Cite
The authors identified a population of Krt17+ basal cells with multipotent properties at the squamo-columnar anorectal junction that maintained a squamous epithelium during normal homeostasis and could participate in the repair of a glandular epithelium following tissue injury.
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Mitoyan, L., Chevrier, V., Hernandez-Vargas, H., Ollivier, A., Homayed, Z., Pannequin, J., Poizat, F., De Biasi-Cador, C., Charafe-Jauffret, E., Ginestier, C., & Guasch, G. (2021). A stem cell population at the anorectal junction maintains homeostasis and participates in tissue regeneration. Nature Communications, 12(1), 2761. https://doi.org/10.1038/s41467-021-23034-x Cite
Scientists summarize studies that indicate the superiority of IL-12 as a potential cytokine for conditioning T cells for adoptive cell therapy.
[Cancer Immunology Immunotherapy]
Investigators highlight the influence of hematopoeitic stem cells in the bone repair process, mainly through the promotion of osteogenesis and angiogenesis at the bone injury site.
[Tissue Engineering Part B-Reviews]
Accelerated Biosciences announced new data that further demonstrates statistically significant cytolysis with iPSc-derived natural killer cells programmed from its ethically sourced hTSCs.
[Accelerated Biosciences (BusinessWire, Inc.)]
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Scientists treated 50 patients with Severe combined immunodeficiency due to adenosine deaminase (ADA) with an investigational gene therapy composed of autologous CD34+ hematopoietic stem and progenitor cells transduced ex vivo with a self-inactivating lentiviral vector encoding human ADA.
[New England Journal of Medicine]
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Kohn, D. B., Booth, C., Shaw, K. L., Xu-Bayford, J., Garabedian, E., Trevisan, V., Carbonaro-Sarracino, D. A., Soni, K., Terrazas, D., Snell, K., Ikeda, A., Leon-Rico, D., Moore, T. B., Buckland, K. F., Shah, A. J., Gilmour, K. C., De Oliveira, S., Rivat, C., Crooks, G. M., … Gaspar, H. B. (2021). Autologous Ex Vivo Lentiviral Gene Therapy for Adenosine Deaminase Deficiency. New England Journal of Medicine, 0(0), null. https://doi.org/10.1056/NEJMoa2027675 Cite
Analysis of CD45RA-depleted haploidentical mixed lymphocytes culture revealed insufficient suppression of alloresponses in the CD4+ compartment and identified CD276 as a marker for alloreactive memory Th1 T cells.
[Bone Marrow Transplantation]
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Hashimoto, H., Kasteleiner, P., Kressin, J., Müller, F., Bühring, H.-J., Handgretinger, R., & Schilbach, K. (2021). Removal of CD276 + cells from haploidentical memory T-cell grafts significantly lowers the risk of GVHD. Bone Marrow Transplantation, 1–19. https://doi.org/10.1038/s41409-021-01307-9 Cite
Researchers engineered red blood cells into artificial antigen-presenting cells presenting a peptide bound to the major histocompatibility complex I, the costimulatory ligand 4-1BBL, and interleukin-12.
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Zhang, X., Luo, M., Dastagir, S. R., Nixon, M., Khamhoung, A., Schmidt, A., Lee, A., Subbiah, N., McLaughlin, D. C., Moore, C. L., Gribble, M., Bayhi, N., Amin, V., Pepi, R., Pawar, S., Lyford, T. J., Soman, V., Mellen, J., Carpenter, C. L., … Chen, T. F. (2021). Engineered red blood cells as an off-the-shelf allogeneic anti-tumor therapeutic. Nature Communications, 12(1), 2637. https://doi.org/10.1038/s41467-021-22898-3 Cite
Off-the-shelf treatments for cancer made from genetically engineered T cells and natural killer, or NK, cells have each shown promise in clinical trials. Two biotech companies- Nkarta and CRISPR Therapeutics- announced a partnership that aims to merge the two immune-cell approaches together.
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