Mesenchymal Cell News

Mesenchymal Cell News is an online resource that delivers updates on the latest research in the mesenchymal stem cell field.

UC Irvine Advances Stem Cell Research with $4 Million CIRM Grant for Shared Resources Lab

[UCI News] The University of California, Irvine (UCI) received a five-year, $4 million grant from the California Institute for Regenerative Medicine to establish a shared resources lab in the Sue & Bill Gross Stem Cell Research Center. The facility will offer essential technologies and training for the development of novel in vitro stem cell-based modeling that will serve researchers across the campus and the state.

CD_99 G1 Neutrophils Modulate Osteogenic Differentiation of Mesenchymal Stem Cells in the Pathological Process of Ankylosing Spondylitis

[Annals Of The Rheumatic Diseases] Single-cell RNA sequencing was used to identify cell populations and their gene signatures in the spinal enthesis of five patients with ankylosing spondylitis and three healthy individuals. The transcriptomes of 40 065 single cells were profiled and divided into 7 clusters: neutrophils, monocytic cells, granulomonocytic progenitor_erythroblasts, T cells, B cells, plasma cells and stromal cells.

Isolation, Identification and Osteogenic Capability Analysis of Mesenchymal Stem Cells Derived From Different Layers of Human Maxillary Sinus Membrane

[Journal Of Clinical Periodontology] Human maxillary sinus membrane (hMSM) was isolated into a monolayer using the combined method of physical separation and enzymatic digestion. The localization of MSCs in hMSM was performed by immunohistological staining and other techniques.

Efficient Preparation of High-Purity and Intact Mesenchymal Stem Cell–Derived Extracellular Vesicles

[Analytical And Bioanalytical Chemistry] Investigators present the adoption of a label-free technique known as “EXODUS” to isolate and purify MSC-extracellular vesicles (EVs) from the conditioned medium. Their findings indicated that EXODUS could rapidly isolate EVs from 10 mL of conditioned medium with a 5-fold higher yield compared to conventional approaches, including ultracentrifugation and polyethylene glycol precipitation methods.

Apoptotic Extracellular Vesicles Derived from Hypoxia-Preconditioned Mesenchymal Stem Cells Within a Modified Gelatine Hydrogel Promote Osteochondral Regeneration by Enhancing Stem Cell Activity and Regulating...

[Journal Of Nanobiotechnology] Researchers investigated whether apoptotic extracellular vesicles (EVs) that were isolated from adipose-derived MSCs cultured under hypoxic conditions exerted greater effects on cartilage repair than those that are isolated from cells cultured under normoxic conditions.

Identification of Critical Process Parameters for Expansion of Clinical Grade Human Wharton’s Jelly-Derived Mesenchymal Stromal Cells in Stirred-Tank Bioreactors

[Biotechnology Journal] The feasibility of transitioning was evaluated from an established Wharton's jelly MSCs 2D production strategy to a new one with stirred-tank bioreactors. Experimental conditions included four GMP-compliant xeno- and serum-free media screened in 2D conditions and two GMP-grade microcarriers assessed in 0.25 L-STRs using serum-containing media.

CYP7B1-Mediated 25-Hydroxycholesterol Degradation Maintains Quiescence-Activation Balance and Improves Therapeutic Potential of Mesenchymal Stem Cells

[Cell Chemical Biology] Scientists demonstrated that CYP7B1 was the common critical molecule that promoted activation and impeded quiescence of bone marrow-MSCs under inflammatory stimulation. Mechanistically, CYP7B1 degraded 25-hydroxycholesterol (25-HC) into 7α,25-dihydroxycholesterol , which alleviates the quiescence maintenance effect of 25-HC through Notch3 signaling pathway activation.

The RNA Binding Proteins LARP4A and LARP4B Promote Sarcoma and Carcinoma Growth and Metastasis

[iScience] The authors revealed that the RNA-binding proteins (RBPs) LARP4A and LARP4B were differentially overexpressed in osteosarcoma and osteosarcoma lung metastases, as well as in prostate cancer.

Mesenchymal Stromal Cell Therapy for COVID-19 Acute Respiratory Distress Syndrome: A Double-Blind Randomised Controlled Trial

[Bone Marrow Transplantation] Scientists conducted a randomised, placebo-controlled, double-blind clinical trial to assess the efficacy and safety of one bone marrow-derived MSC infusion in twenty patients with moderate to severe ARDS caused by COVID-19.

3D Culture of Alginate-Hyaluronic Acid Hydrogel Supports the Stemness of Human Mesenchymal Stem Cells

[Scientific Reports] Researchers developed natural biomaterials, including hyaluronic acid, alginate, and gelatin, to mimic the creation of a 3D human MSC extracellular environment and selected hydrogels with high proliferation capacity for 3D MSC culture.

Boosting Cartilage Repair with Silk Fibroin-DNA Hydrogel-Based Cartilage Organoid Precursor

[Bioactive Materials] Researchers developed a novel RGD-SF-DNA hydrogel microsphere via a microfluidic system by integrating photopolymerization with self-assembly techniques and then modified with Pep-RGDfKA. In vitro studies demonstrated that RSD-MSs enhanced BMSCs proliferation, adhesion, and chondrogenic differentiation.

Effects of Bone Marrow Mesenchymal Stem Cell-Derived Exosomes in Central Nervous System Diseases

[Molecular Neurobiology] The authors review the mechanisms of BMSC-Exos in central nervous system diseases and provide direction for further research.

Mesenchymal Cell News covers topics such as the isolation, identification, and culturing conditions of mesenchymal stem cells, as well as their cellular characterization, behavior and differentiation capacities. We also include research that focuses on identifying tissue sources of mesenchymal stem cells and their potential therapeutic applications in regenerative medicine and cellular therapies.