Identification of SARS-CoV-2 Inhibitors using Lung and Colonic Organoids

As SARS-CoV-2 primarily infects the respiratory tract, investigators developed a lung organoid model using human pluripotent stem cells, and generated complementary hPSC-derived colonic organoids to explore the response of colonic cells to SARS-CoV-2 infection.
[Nature]
Han, Y., Duan, X., Yang, L., Nilsson-Payant, B. E., Wang, P., Duan, F., Tang, X., Yaron, T. M., Zhang, T., Uhl, S., Bram, Y., Richardson, C., Zhu, J., Zhao, Z., Redmond, D., Houghton, S., Nguyen, D.-H. T., Xu, D., Wang, X., … Chen, S. (2020). Identification of SARS-CoV-2 Inhibitors using Lung and Colonic Organoids. Nature, 1–8. https://doi.org/10.1038/s41586-020-2901-9 Cite
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Optimal, Large-Scale Propagation of Mouse Mammary Tumor Organoids

Scientists identified critical factors regulating organoid growth ex vivo, and to use these observations to develop a more efficient organoid expansion method.
[Journal of Mammary Gland Biology and Neoplasia]
Wrenn, E. D., Moore, B. M., Greenwood, E., McBirney, M., & Cheung, K. J. (2020). Optimal, Large-Scale Propagation of Mouse Mammary Tumor Organoids. Journal of Mammary Gland Biology and Neoplasia. https://doi.org/10.1007/s10911-020-09464-1 Cite
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Modeling Cancer Progression using Human Pluripotent Stem Cell-Derived Cells and Organoids

The authors review the application of human pluripotent stem cell-derived organoid technologies in cancer modeling with respect to the cell-of-origin, cancer propagation, and metastasis.
[Stem Cell Research]
Zhang, M., Jeya Vandana, J., Lacko, L., & Chen, S. (2020). Modeling Cancer Progression using Human Pluripotent Stem Cell-Derived Cells and Organoids. Stem Cell Research, 102063. https://doi.org/10.1016/j.scr.2020.102063 Cite
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Optimal, Large-Scale Propagation of Mouse Mammary Tumor Organoids

Using time-lapse imaging of mouse mammary tumor organoids in 3D culture, researchers observed that outgrowth potential varied non-linearly with initial organoid size.
[Journal of Mammary Gland Biology and Neoplasia]
Wrenn, E. D., Moore, B. M., Greenwood, E., McBirney, M., & Cheung, K. J. (2020). Optimal, Large-Scale Propagation of Mouse Mammary Tumor Organoids. Journal of Mammary Gland Biology and Neoplasia. https://doi.org/10.1007/s10911-020-09464-1 Cite
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Intestinal Organoid/Enteroid-Based Models for Cryptosporidium

Scientists discuss recent breakthroughs in the field and highlight different models for functional ex vivo organoid or enteroidderived culture systems.
[Current Opinion in Microbiology]
Bhalchandra, S., Lamisere, H., & Ward, H. (2020). Intestinal organoid/enteroid-based models for Cryptosporidium. Current Opinion in Microbiology, 58, 124–129. https://doi.org/10.1016/j.mib.2020.10.002 Cite
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Development and Characterization of Rat Duodenal Organoids for ADME and Toxicology Applications

Investigators optimized and characterized rat duodenal organoids with light and electron microscopy, immunofluorescence and notably, global mRNA expression.
[Toxicology]
Hedrich, W. D., Panzica-Kelly, J. M., Chen, S.-J., Strassle, B., Hasson, C., Lecureux, L., Wang, L., Chen, W., Sherry, T., Gan, J., & Davis, M. (2020). Development and characterization of rat duodenal organoids for ADME and toxicology applications. Toxicology, 152614. https://doi.org/10.1016/j.tox.2020.152614 Cite
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Generation of a Transplantable Population of Human iPSC-Derived Retinal Ganglion Cells

Researchers describe the generation of functional retinal ganglion cells (RGCs) from iPSCs based on innovative 3D/2D stepwise differentiation protocol. They demonstrated that targeting the cell surface marker THY1 was an effective strategy to select transplantable RGCs.
[Frontiers in Cell and Developmental Biology]
Rabesandratana, O., Chaffiol, A., Mialot, A., Slembrouck-Brec, A., Joffrois, C., Nanteau, C., Rodrigues, A., Gagliardi, G., Reichman, S., Sahel, J.-A., Chédotal, A., Duebel, J., Goureau, O., & Orieux, G. (2020). Generation of a Transplantable Population of Human iPSC-Derived Retinal Ganglion Cells. Frontiers in Cell and Developmental Biology, 8. https://doi.org/10.3389/fcell.2020.585675 Cite
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NMR Microsystem for Label-Free Characterization of 3D Nanoliter Microtissues

Scientists introduce a novel microsystem, which combines CMOS technology with 3D microfabrication, enabling nL NMR as a platform tool for non-invasive spectroscopy of organoids, 3D cell cultures, and early stage embryos.
[Scientific Reports]
Grisi, M., Conley, G. M., Rodriguez, K. J., Riva, E., Egli, L., Moritz, W., Lichtenberg, J., Brugger, J., & Boero, G. (2020). NMR microsystem for label-free characterization of 3D nanoliter microtissues. Scientific Reports, 10(1), 18306. https://doi.org/10.1038/s41598-020-75480-0 Cite
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Interplay Among p21Waf1/Cip1, MUSASHI-1 and Krüppel-Like Factor 4 in Activation of Bmi1-CreER Reserve Intestinal Stem Cells After Gamma Radiation-Induced Injury

Bmi1-specific Klf4 deletion resulted in decreased numbers of MSI1+ cells in regenerating crypts compared to those of control mice. Researchers showed that KLF4 bound to the Msi1 promoter and activated its expression in vitro.
[Scientific Reports]
Interplay among p21 Waf1/Cip1 , MUSASHI-1 and Krüppel-like factor 4 in activation of Bmi1-Cre ER reserve intestinal stem cells after gamma radiation-induced injury | Scientific Reports. (n.d.). Retrieved October 27, 2020, from https://www.nature.com/articles/s41598-020-75171-w Cite
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Prime Editing for Functional Repair in Patient-Derived Disease Models

Prime editing functionally recovered disease-causing mutations in intestinal organoids from patients with DGAT1-deficiency and liver organoids from a patient with Wilson disease
[Nature Communications]
Schene, I. F., Joore, I. P., Oka, R., Mokry, M., van Vugt, A. H. M., van Boxtel, R., van der Doef, H. P. J., van der Laan, L. J. W., Verstegen, M. M. A., van Hasselt, P. M., Nieuwenhuis, E. E. S., & Fuchs, S. A. (2020). Prime editing for functional repair in patient-derived disease models. Nature Communications, 11(1), 5352. https://doi.org/10.1038/s41467-020-19136-7 Cite
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Exogenous L-Arginine Increases Intestinal Stem Cell Function through CD90+ Stromal Cells Producing mTORC1-Induced Wnt2b

Scientists utilized mice and small intestinal organoid models to clarify the role of L-arginine on epithelial differentiation of intestinal stem cells (ISCs). They showed that L-arginine increased expansion of ISCs in mice.
[Communications Biology]
Hou, Q., Dong, Y., Huang, J., Liao, C., Lei, J., Wang, Y., Lai, Y., Bian, Y., He, Y., Sun, J., Sun, M., Jiang, Q., Wang, B., Yu, Z., Guo, Y., & Zhang, B. (2020). Exogenous L-arginine increases intestinal stem cell function through CD90+ stromal cells producing mTORC1-induced Wnt2b. Communications Biology, 3(1), 1–16. https://doi.org/10.1038/s42003-020-01347-9 Cite
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