Organoid News

Organoid News is an online resource dedicated to the latest research about and featuring organoids.

Tissue- and Cell-Type-Specific Molecular and Functional Signatures of 16p11.2 Reciprocal Genomic Disorder Across Mouse Brain and Human Neuronal Models

[American Journal of Human Genetics] Researchers performed transcriptome profiling of 350 libraries from six tissues in mouse models harboring copy-number variants (CNVs) of the syntenic 7qF3 region, as well as cellular, transcriptional, and single-cell analyses in 54 isogenic neural stem cell, induced neuron, and cerebral organoid models of CRISPR-engineered 16p11.2 CNVs.

Retinoic Acid Delays Initial Photoreceptor Differentiation and Results in a Highly Structured Mature Retinal Organoid

[Stem Cell Research & Therapy] A 2D-3D differentiation method was used to generate retinal organoids, which were cultured with or without the most commonly used media supplements, notably retinoic acid.

TGFβ Superfamily Signaling Regulates the State of Human Stem Cell Pluripotency and Capacity To Create Well-Structured Telencephalic Organoids

[Stem Cell Reports] Scientists demonstrated that a major contributor to cortical organoid quality is the way hPSCs are maintained prior to differentiation.

Dual Roles of β-Arrestin 1 in Mediating Cell Metabolism and Proliferation in Gastric Cancer

[Proceedings of the National Academy of Sciences of the United States of America] The authors identified that ARRB1 played essential roles in mediating gastric cancer cell metabolism and proliferation, by combining cohort analysis and functional investigation using patient-derived preclinical models.

Novel Histone Deacetylase 6 Inhibitor Confers Anti-Inflammatory Effects and Enhances Gut Barrier Function

[Gut and Liver] The effects of CKD-506 on gut barrier function in a cell line and colon organoids, based on examinations of mRNA production, goblet cell differentiation, and E-cadherin recovery, were investigated using quantitative reverse transcription PCR, immunofluorescence, and a fluorescein isothiocyanate-dextran permeability assay.

DNA Sensor Associated Type I Interferon Signaling Is Increased in Ulcerative Colitis and Induces JAK-Dependent Inflammatory Cell Death in Colonic Organoids

[American Journal of Physiology-Gastrointestinal and Liver Physiology] The effects of type I IFN, IFN-γ, and TNF-α on gene expression, cytokine production and cell death were investigated in human colonic organoids.

Crown Bioscience & MBL Launch Joint Venture To Serve Japanese Biopharmaceutical Community

[Crown Bioscience] Crown Bioscience & MBL, a joint venture between two JSR Life Sciences companies formed earlier this year, announced the launch of their full-service drug discovery and development capabilities in Japan.

Engineering Placenta-Like Organoids Containing Endogenous Vascular Cells from Human-Induced Pluripotent Stem Cells

[Bioengineering & Translational Medicine] Researchers presented a new strategy to establish placenta-like organoids with vascular-like structures from human-induced pluripotent stem cells in a defined three-dimensional culture system.

Kidney Organoids Generated Using an Allelic Series of NPHS2 Point Variants Reveal Distinct Intracellular Podocin Mistrafficking

[Journal of the American Society of Nephrology] Investigators generated a series of human iPSC lines bearing pathogenic missense variants of NPHS2, encoding the protein changes p.G92C, p.P118L, p.R138Q, p.R168H and p.R291W, as well as control lines.

Applications of Human Organoids in the Personalized Treatment for Digestive Diseases

[Signal Transduction and Targeted Therapy] Over the past few decades, the use of organoids has led to an advanced understanding of the composition of each digestive organ and has facilitated disease modeling, chemotherapy dose prediction, CRISPR-Cas9 genetic intervention, high-throughput drug screening, and identification of SARS-CoV-2 targets, pathogenic infection.

3D In Vitro Cultures of Human Bone Marrow and Wharton’s Jelly Derived Mesenchymal Stromal Cells Show High Chondrogenic Potential

[Frontiers in Bioengineering and Biotechnology] Chondrogenic potentials of 3D high-density cultures of Bone Marrow and Wharton’s Jelly-derived MSCs was investigated by chondrogenesis- and cytokine-related gene expression over a 16-day culture period supplemented with human transforming growth factor.

Autophagic State Prospectively Identifies Facultative Stem Cells in the Intestinal Epithelium

[EMBO Reports] Researchers utilized in vivo labeling combined with ex vivo organoid formation assays to evaluate how cell age and autophagic state contributed to facultative stem cell activity within secretory lineages.

Organoids are three-dimensional cell cultures that more accurately model cell behavior, organ function, and pathology than traditional two-dimensional cell culture. Organoid News was launched in 2020 to help scientists stay up-to-date with this revolutionary new research system. Use Organoid News to stay current with the latest applications and discoveries using organoids, as well as new reviews, jobs, news, and upcoming events.