The Microfluidic Environment Reveals a Hidden Role of Self-Organizing Extracellular Matrix in Hepatic Commitment and Organoid Formation of hiPSCs

Hepatic progenitor cells either derived in microfluidics or exposed to exogenous ECM stimuli showed a significantly higher potential of forming hepatic organoids that could be rapidly expanded for several passages and further differentiated into functional hepatocytes.
[Cell Reports]
Michielin, F., Giobbe, G. G., Luni, C., Hu, Q., Maroni, I., Orford, M. R., Manfredi, A., Filippo, L. D., David, A. L., Cacchiarelli, D., Coppi, P. D., Eaton, S., & Elvassore, N. (2020). The Microfluidic Environment Reveals a Hidden Role of Self-Organizing Extracellular Matrix in Hepatic Commitment and Organoid Formation of hiPSCs. Cell Reports, 33(9). https://doi.org/10.1016/j.celrep.2020.108453 Cite
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Antigen Retrieval and Clearing for Whole-Organ Immunofluorescence by FLASH

FLASH utilizes non-degradative epitope recovery and membrane solubilization to enable the detection of a multitude of membranous, cytoplasmic and nuclear antigens in whole mouse organs and embryos, human biopsies, organoids and Drosophila.
[Nature Protocols]
Messal, H. A., Almagro, J., Zaw Thin, M., Tedeschi, A., Ciccarelli, A., Blackie, L., Anderson, K. I., Miguel-Aliaga, I., van Rheenen, J., & Behrens, A. (2020). Antigen retrieval and clearing for whole-organ immunofluorescence by FLASH. Nature Protocols, 1–33. https://doi.org/10.1038/s41596-020-00414-z Cite
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Patient-Derived Glioblastoma Stem cells transfer mitochondria through Tunneling Nanotubes in Tumor Organoids

Both glioblastoma stem cells, grown in classical 2D culture and in 3D-tumor organoids, formed functional tunneling nanotubes which allowed mitochondria transfer.
[Biochemical Journal]
Pinto, G., Saenz de Santa Maria, I., Chastagner, P., Perthame, E., Delmas, C., Toulas, C., Moyal-Jonathan-Cohen, E., Brou, C., & Zurzolo, C. (n.d.). Patient-derived Glioblastoma Stem cells transfer mitochondria through Tunneling Nanotubes in Tumor Organoids. Biochemical Journal. https://doi.org/10.1042/BCJ20200710 Cite
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A Comprehensive Library of Human Transcription Factors for Cell Fate Engineering

Scientists demonstrated orthogonal programming by including oligodendrocyte-inducible hPSCs with unmodified hPSCs to generate cerebral organoids, which expedited in situ myelination.
[Nature Biotechnology]
Ng, A. H. M., Khoshakhlagh, P., Rojo Arias, J. E., Pasquini, G., Wang, K., Swiersy, A., Shipman, S. L., Appleton, E., Kiaee, K., Kohman, R. E., Vernet, A., Dysart, M., Leeper, K., Saylor, W., Huang, J. Y., Graveline, A., Taipale, J., Hill, D. E., Vidal, M., … Church, G. M. (2020). A comprehensive library of human transcription factors for cell fate engineering. Nature Biotechnology, 1–10. https://doi.org/10.1038/s41587-020-0742-6 Cite
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Directed Induction of Alveolar Type I Cells Derived from Pluripotent Stem Cells via Wnt Signaling Inhibition

Scientists performed single‐cell RNA sequencing of fibroblast‐dependent alveolar organoids, including human induced pluripotent stem cell‐derived epithelial cells and fetal lung fibroblasts, and identified hiPSC‐derived AT1 cells.
[Stem Cells]
Directed induction of alveolar type I cells derived from pluripotent stem cells via Wnt signaling inhibition - Kanagaki - - STEM CELLS - Wiley Online Library. (n.d.). Retrieved November 26, 2020, from https://stemcellsjournals.onlinelibrary.wiley.com/doi/10.1002/stem.3302 Cite
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Shared Extracellular Vesicle miRNA Profiles of Matched Ductal Pancreatic Adenocarcinoma Organoids and Blood Plasma Samples Show the Power of Organoid Technology

Researchers showed that the miRNA cargo of extracellular vesicles (EVs) from pancreatic ductal adenocarcinoma organoids largely differs among patients. However, they detected a common set of EV miRNAs that were present in matched organoids and blood plasma samples of individual patients.
[Cellular and Molecular Life Sciences]
Zeöld, A., Sándor, G. O., Kiss, A., Soós, A. Á., Tölgyes, T., Bursics, A., Szűcs, Á., Harsányi, L., Kittel, Á., Gézsi, A., Buzás, E. I., & Wiener, Z. (2020). Shared extracellular vesicle miRNA profiles of matched ductal pancreatic adenocarcinoma organoids and blood plasma samples show the power of organoid technology. Cellular and Molecular Life Sciences. https://doi.org/10.1007/s00018-020-03703-8 Cite
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Definitive Hematopoietic Stem/Progenitor Cells from Human Embryonic Stem Cells through Serum/Feeder-Free Organoid-Induced Differentiation

To derive definitive hematopoietic stem/precursor cells from human embryonic stem cells, scientists differentiated mesodermally specified embryoid bodies on gelatin-coated plates in serum/feeder-free conditions.
[Stem Cell Research & Therapy]
Demirci, S., Haro-Mora, J. J., Leonard, A., Drysdale, C., Malide, D., Keyvanfar, K., Essawi, K., Vizcardo, R., Tamaoki, N., Restifo, N. P., Tisdale, J. F., & Uchida, N. (2020). Definitive hematopoietic stem/progenitor cells from human embryonic stem cells through serum/feeder-free organoid-induced differentiation. Stem Cell Research & Therapy, 11(1), 493. https://doi.org/10.1186/s13287-020-02019-5 Cite
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Unveiling Role of Sphingosine-1-Phosphate Receptor 2 as a Brake of Epithelial Stem Cell Proliferation and a Tumor Suppressor in Colorectal Cancer

Researchers mimicked the ablation of S1PR2 in normal mucosa by treating S1PR2+/+ organoids with JTE013 and characterized intestinal epithelial stem cells isolated from S1PR2-/-Lgr5-EGFP- mice.
[Journal of Experimental & Clinical Cancer Research]
Petti, L., Rizzo, G., Rubbino, F., Elangovan, S., Colombo, P., Restelli, S., Piontini, A., Arena, V., Carvello, M., Romano, B., Cavalleri, T., Anselmo, A., Ungaro, F., D’Alessio, S., Spinelli, A., Stifter, S., Grizzi, F., Sgambato, A., Danese, S., … Vetrano, S. (2020). Unveiling role of sphingosine-1-phosphate receptor 2 as a brake of epithelial stem cell proliferation and a tumor suppressor in colorectal cancer. Journal of Experimental & Clinical Cancer Research, 39(1), 253. https://doi.org/10.1186/s13046-020-01740-6 Cite
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Establishment of Human Fetal Hepatocyte Organoids and CRISPR–Cas9-Based Gene Knockin and Knockout in Organoid Cultures from Human Liver

Scientists established culture conditions that facilitate long-term expansion of human fetal hepatocytes as organoids
[Nature Protocols]
Hendriks, D., Artegiani, B., Hu, H., Chuva de Sousa Lopes, S., & Clevers, H. (2020). Establishment of human fetal hepatocyte organoids and CRISPR–Cas9-based gene knockin and knockout in organoid cultures from human liver. Nature Protocols, 1–36. https://doi.org/10.1038/s41596-020-00411-2 Cite
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Organoid Sample Preparation and Extraction for LC-MS Peptidomics

Scientists describe the peptidomic analysis of organoid lysates, FACS-purified cell populations, and 2D culture secretions by liquid chromatography mass spectrometry.
[STAR Protocols]
Miedzybrodzka, E. L., Foreman, R. E., Galvin, S. G., Larraufie, P., George, A. L., Goldspink, D. A., Reimann, F., Gribble, F. M., & Kay, R. G. (2020). Organoid Sample Preparation and Extraction for LC-MS Peptidomics. STAR Protocols, 100164. https://doi.org/10.1016/j.xpro.2020.100164 Cite
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Generation of Brain Organoids from Mouse ESCs via Teratoma Formation

Investigators aimed to establish a new method to generate brain organoids efficiently in a mouse model. They applied the in vivo teratoma formation method as a new approach to generate brain organoids.
[Stem Cell Research]
Ji Lee, W., Eon Lee, J., Ju Hong, Y., Hoon Yoon, S., Song, H., Park, C., Hong, K., Choi, Y., & Tae Do, J. (2020). Generation of brain organoids from mouse ESCs via teratoma formation. Stem Cell Research, 102100. https://doi.org/10.1016/j.scr.2020.102100 Cite
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