A Genome-Scale CRISPR Screen Reveals Factors Regulating Wnt-Dependent Renewal of Mouse Gastric Epithelial Cells

Scientists investigated the in vivo role of Dhx15 in intestinal antibacterial responses by generating mice that were intestinal epithelial cell-specific deficient for Dhx15.
[Proceedings of the National Academy of Sciences of the United States of America]
Murakami, K., Terakado, Y., Saito, K., Jomen, Y., Takeda, H., Oshima, M., & Barker, N. (2021). A genome-scale CRISPR screen reveals factors regulating Wnt-dependent renewal of mouse gastric epithelial cells. Proceedings of the National Academy of Sciences, 118(4). https://doi.org/10.1073/pnas.2016806118 Cite
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Stromal DLK1 Promotes Proliferation and Inhibits Differentiation of the Intestinal Epithelium during Development

Scientists used a novel co-culture system with E14.5 intestinal epithelial organoids to examine the microenvironmental cues that regulate the embryonic stem/progenitor population, focusing on the role of Notch pathway factor, Delta-Like Protein 1.
[American Journal of Physiology-Gastrointestinal and Liver Physiology]
Ichinose, M., Suzuki, N., Wang, T., Wright, J. A., Lannagan, T. R., Vrbanac, L., Kobayashi, H., Gieniec, K. A., Ng, J. Q., Hayakawa, Y., García-Gallastegui, P., Monsalve, E. M., Bauer, S. R., Laborda, J., García-Ramírez, J. J., Ibarretxe, G., Worthley, D. L., & Woods, S. L. (2021). Stromal DLK1 promotes proliferation and inhibits differentiation of the intestinal epithelium during development. American Journal of Physiology-Gastrointestinal and Liver Physiology. https://doi.org/10.1152/ajpgi.00445.2020 Cite
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An Endometrial Organoid Model of Chlamydia-Epithelial and Immune Cell Interactions

Researchers describe a primary organoid system derived from endometrial tissue to recapitulate epithelial cell diversity, polarity, and ensuing responses to Chlamydia infection.
[Journal of Cell Science]
Dolat, L., & Valdivia, R. H. (2021). An endometrial organoid model of Chlamydia-epithelial and immune cell interactions. Journal of Cell Science. https://doi.org/10.1242/jcs.252403 Cite
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Pretreatment of Aged Mice with Retinoic Acid Supports Alveolar Regeneration via Upregulation of Reciprocal PDGFA Signaling

In vitro organoids generated from epithelial cells of mouse or patient with idiopathic pulmonary fibrosis co-cultured with young, aged or retinoic acid-pretreated murine fibroblasts were used to test potential targets.
[Thorax]
Gokey, J. J., Snowball, J., Green, J., Waltamath, M., Spinney, J. J., Black, K. E., Hariri, L. P., Xu, Y., & Perl, A. K. (2021). Pretreatment of aged mice with retinoic acid supports alveolar regeneration via upregulation of reciprocal PDGFA signalling. Thorax. https://doi.org/10.1136/thoraxjnl-2020-214986 Cite
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Biomaterial-Guided Stem Cell Organoid Engineering for Modeling Development and Diseases

The authors discuss the enhancement of organoid systems for therapeutic applications using biofabrication and organoid-on-chip platforms, which facilitate the assembly of complex organoid systems for in vitro modeling of development and diseases.
[Acta Biomaterialia]
Hoang, P., & Ma, Z. (2021). Biomaterial-guided stem cell organoid engineering for modeling development and diseases. Acta Biomaterialia. https://doi.org/10.1016/j.actbio.2021.01.026 Cite
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3D High-Density Microelectrode Array with Optical Stimulation and Drug Delivery for Investigating Neural Circuit Dynamics

Researchers present a 3D high-density multifunctional microelectrode array with optical stimulation and drug delivery for investigating neural circuit dynamics within engineered 3D neural tissues.
[Nature Communications]
3D high-density microelectrode array with optical stimulation and drug delivery for investigating neural circuit dynamics | Nature Communications. (n.d.). Retrieved January 22, 2021, from https://www.nature.com/articles/s41467-020-20763-3 Cite
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Epithelium-Derived Indian Hedgehog Restricts Stromal Expression of ErbB Family Members That Drive Colonic Tumor Cell Proliferation

Scientists found that recombinant EREG, BTC, and NRG1 but not Lgr5 ligand R-Spondin promoted growth and proliferation of Apc double mutant colonic organoids.
[Oncogene]
Westendorp, F., Karpus, O. N., Koelink, P. J., Vermeulen, J. L. M., Meisner, S., Koster, J., Büller, N. V. J. A., Wildenberg, M. E., Muncan, V., & van den Brink, G. R. (2021). Epithelium-derived Indian Hedgehog restricts stromal expression of ErbB family members that drive colonic tumor cell proliferation. Oncogene, 1–16. https://doi.org/10.1038/s41388-020-01633-0 Cite
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Re-Expression of REG Family and DUOXs Genes in CRC Organoids by Co-Culturing With CAFs

Researchers found that the expression levels of several genes, which were highly expressed in original colorectal carcinoma tissues, were downregulated in organoids but re-expressed in organoids by co-culturing with cancer-associated fibroblasts.
[Scientific Reports]
Naruse, M., Ochiai, M., Sekine, S., Taniguchi, H., Yoshida, T., Ichikawa, H., Sakamoto, H., Kubo, T., Matsumoto, K., Ochiai, A., & Imai, T. (2021). Re-expression of REG family and DUOX s genes in CRC organoids by co-culturing with CAFs. Scientific Reports, 11(1), 2077. https://doi.org/10.1038/s41598-021-81475-2 Cite
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Transit-Amplifying Cells Coordinate Changes in Intestinal Epithelial Cell-Type Composition

Using a high-throughput approach that combines enteroid monolayers and quantitative imaging, scientists identified conditions that enrich for specific cell types as well as interactions between pathways.
[Developmental Cell]
Sanman, L. E., Chen, I. W., Bieber, J. M., Steri, V., Trentesaux, C., Hann, B., Klein, O. D., Wu, L. F., & Altschuler, S. J. (2021). Transit-Amplifying Cells Coordinate Changes in Intestinal Epithelial Cell-Type Composition. Developmental Cell, 0(0). https://doi.org/10.1016/j.devcel.2020.12.020 Cite
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Epitope-Resolved Profiling of the SARS-CoV-2 Antibody Response Identifies Cross-Reactivity with Endemic Human Coronaviruses

Researchers studied cross-reactivity using a highly multiplexed peptide assay to generate an epitope-resolved view of IgG reactivity across all human coronaviruses in both COVID-19 convalescent and negative donors.
[Cell Reports Medicine]
Ladner, J. T., Henson, S. N., Boyle, A. S., Engelbrektson, A. L., Fink, Z. W., Rahee, F., D’ambrozio, J., Schaecher, K. E., Stone, M., Dong, W., Dadwal, S., Yu, J., Caligiuri, M. A., Cieplak, P., Bjørås, M., Fenstad, M. H., Nordbø, S. A., Kainov, D. E., Muranaka, N., … Altin, J. A. (2021). Epitope-resolved profiling of the SARS-CoV-2 antibody response identifies cross-reactivity with endemic human coronaviruses. Cell Reports Medicine, 2(1). https://doi.org/10.1016/j.xcrm.2020.100189 Cite
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Advances in CNS Organoids: A Focus on Organoid-Based Models for Motor Neuron Disease

Scientists review recent advances in the development of CNS organoid models, their application in neurodegenerative diseases and technical limitations.
[Tissue Engineering Part C: Methods]
Vieira de Sa, R., Canizares Luna, M., & Pasterkamp, J. (2021). Advances in CNS organoids: a focus on organoid-based models for motor neuron disease. Tissue Engineering Part C: Methods. https://doi.org/10.1089/ten.TEC.2020.0337 Cite
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