Keep Current with the Latest in Cell Biology Research

A Quadri-Fluorescence SARS-CoV-2 Pseudovirus System for Efficient Antigenic Characterization of Multiple Circulating Variants

[Cell Reports Methods] Scientists engineered a quadri-fluorescence lentiviral pseudovirus system employing four spectrally distinct fluorescent protein reporters, enabling the concurrent measurement of neutralizing antibodies against four SARS-CoV-2 variants within a single assay.

Discovery of CO2 Tolerance Genes Associated with Virulence in the Fungal Pathogen Cryptococcus neoformans

[Nature Microbiology] Researchers utilized quantitative trait loci mapping with 374 progeny from a cross between a CO2-tolerant clinical isolate and a CO2-sensitive environmental isolate to identify genetic regions regulating CO2 tolerance.

Mycobacterium tuberculosis Virulence Lipid PDIM Inhibits Autophagy in Mice

[Nature Microbiology] The authors used transposon sequencing analysis of Mycobacterium tuberculosis (Mtb) in wild-type and autophagy-deficient mouse macrophages lacking ATG5 or ATG7, and found that Mtb genes involved in phthiocerol dimycocerosate (PDIM) virulence lipid synthesis confered resistance to autophagy.

Cell Wall Melanin Impedes Growth of the Cryptococcus neoformans Polysaccharide Capsule by Sequestering Calcium

[Proceedings of The National Academy of Sciences of The United States of America] Investigators reported an interaction between the two major cryptococcal virulence factors, the polysaccharide capsule and melanin.

Leishmania infantum Exploits the Anti-Ferroptosis Effects of Nrf2 to Escape Cell Death in Macrophages

[Cell Reports] Researchers reported that the activation of the transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2) in macrophages played a pivotal role in the progression of Leishmania infantum infection by controlling inflammation and redox balance of macrophages.

Necrotizing Soft Tissue Infections

[Jama Surgery] Investigators discuss diagnostic and treatment approaches to necrotizing soft tissue infection (NSTI) while highlighting future directions and promising developments in NSTI management.

NIH Awards Will Support Innovation in Syphilis Diagnostics

[National Institutes of Health] The National Institutes of Health’s (NIH) National Institute of Allergy and Infectious Diseases has awarded grants for 10 projects to improve diagnostic tools for congenital and adult syphilis—conditions currently diagnosed with a sequence of tests, each with limited precision.

Generation of an Isogenic Hereditary Hemorrhagic Telangiectasia Model via Prime Editing in Human Induced Pluripotent Stem Cells

[International Journal of Stem Cells] Mice and human umbilical vein endothelial cell models did not sufficiently elucidate the relationship between the genotype and disease phenotype in hereditary hemorrhagic telangiectasia 1, demanding more clinically relevant models that reflect human genetics. Therefore, the authors used prime editing to propose a method for establishing an isogenic hiPSC line.

Introduction of an RS1 Mutation Causative Variant Consistent with Identified XLRS Patient Using CRISPR/Cas9 Technology in Normal iPSC

[Stem Cell Research] Researchers introduced the RS1 (c.C304T, p.R102W) mutation into a normal iPSC line using CRISPR/Cas9 technology.

Mitochondrial Dysfunction and Increased Reactive Oxygen Species Production in MECP2 Mutant Astrocytes and Their Impact on Neurons

[Scientific Reports] Using hESC lines, scientists modeled MECP2 loss-of-function to explore its effects on astrocyte development and dysfunction in the brain.

Tracking Induced Pluripotent Stem Cell Differentiation with a Fluorescent Genetically Encoded Epigenetic Probe

[Cellular and Molecular Life Sciences] Investigators presented a genetically encoded fluorescent probe used to track changes in H3K9me3 landscapes during the differentiation of iPSCs into induced neurons.

The Pluripotent Path to Immunotherapy

[Experimental Hematology] iPSCs hold the potential to address challenges with utilizing adoptive cell therapy as cancer treatment, as they can provide an unlimited source for the in vitro generation of immune cells.

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