Keep Current with the Latest in Cell Biology Research
The authors report that mouse ESCs with Wnt3a-bead induction displayed nonoverlapping preexisting (old) versus newly synthesized (new) histone H3 patterns, but mouse ESCs without Wnt3a beads had largely overlapping patterns.
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Human PSCs were subject to directed differentiation through a paraxial mesoderm progenitor state that generates brown adipocytes (BAs) at high efficiency. Molecular analysis identified potential regulatory networks for BA development, giving insight into development along this lineage.
[Cell Stem Cell]
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The anti-tumor effect of a water extract of G. lucidum was investigated using brain tumor cells. Researchers used an analysis of cell viability, flow cytometry, the IncuCyte live-cell analysis system, and Western blotting to study its effects.
Scientists report that an identified Lymnaea neuron selectively released its classical small molecule and peptide neurotransmitters, acetylcholine and FMRFamide-derived neuropeptides, to differentially influence the activity of distinct postsynaptic targets that coordinated cardiorespiratory behaviour.
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Getz, A. M., Janes, T. A., Visser, F., Zaidi, W., & Syed, N. I. (2020). Neurotrophic factors and target-specific retrograde signaling interactions define the specificity of classical and neuropeptide cotransmitter release at identified Lymnaea synapses. Scientific Reports, 10(1), 13526. https://doi.org/10.1038/s41598-020-70322-5 Cite
Depletion of embryonic ectoderm development (EED), a core polycomb repressive complex 2 subunit, reduced differentiation of oligodendrocyte progenitors (OPCs), and caused an OPC-to-astrocyte fate switch in a region-specific manner.
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Wang, J., Yang, L., Dong, C., Wang, J., Xu, L., Qiu, Y., Weng, Q., Zhao, C., Xin, M., & Lu, Q. R. (2020). EED-mediated histone methylation is critical for CNS myelination and remyelination by inhibiting WNT, BMP, and senescence pathways. Science Advances, 6(33), eaaz6477. https://doi.org/10.1126/sciadv.aaz6477 Cite
Tumor-derived DNA isolated from Lewis cell lines was transfected into microglia, and analyzed for stimulator of interferon genes (STING) effects.
[Journal of Ethnopharmacology]
[Proceedings of the National Academy of Sciences of the United States of America]
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Liu, J., Huang, Z.-P., Nie, M., Wang, G., Silva, W. J., Yang, Q., Freire, P. P., Hu, X., Chen, H., Deng, Z., Pu, W. T., & Wang, D.-Z. (2020). Regulation of myonuclear positioning and muscle function by the skeletal muscle-specific CIP protein. Proceedings of the National Academy of Sciences, 117(32), 19254–19265. https://doi.org/10.1073/pnas.1922911117 Cite
Researchers used intersectional genetic strategy to ablate NPY+ noradrenergic neurons and/or adrenal chromaffin cells.
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The co-immobilized peptide surfaces showed anti-biofilm activity against Streptococcus gordonii while enhancing proliferation, hemidesmosome formation, and mechanical attachment of orally-derived keratinocytes.
[ACS Biomaterials Science & Engineering]
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Investigators demonstrated that treatment with phorbol 12-myristate 13-acetate for six hours significantly increased acid-sensing ion channel 1a (ASIC1a) protein expression and ASIC currents in NS20Y cells, a neuronal cell line, and in primary cultured mouse cortical neurons.
Cortical brain organoid modeling showed reduced proliferation of radial glial cells, leading to smaller organoids characteristic of microcephaly.
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Wang, L., Li, Z., Sievert, D., Smith, D. E. C., Mendes, M. I., Chen, D. Y., Stanley, V., Ghosh, S., Wang, Y., Kara, M., Aslanger, A. D., Rosti, R. O., Houlden, H., Salomons, G. S., & Gleeson, J. G. (2020). Loss of NARS1 impairs progenitor proliferation in cortical brain organoids and leads to microcephaly. Nature Communications, 11(1), 4038. https://doi.org/10.1038/s41467-020-17454-4 Cite
Investigators demonstrated the production of cardiomyocytes by using high definition direct writing technologies to influence embryoid bodies aggregation from stem cells.