[Scientific Data] Researchers generated a single-cell RNA sequencing time course to study the role of developmental signaling pathways on multilineage diversification from pluripotency in vitro.

[Journal of The American Chemical Society] Scientists reported a heparan sulfate mimetic containing a DNA aptamer with affinity for the membrane-associated pluripotency marker, alkaline phosphatase, that can be selectively targeted to the surface of ESCs.

[Science] Investigators modulated developmental signaling pathways combined with transcripiton factor overexpression to explore the spectrum of neuron subtypes generated from PSCs.

[Genetics] To dissect the molecular mechanisms underlying aberrant function of BCL-6 corepressor (BCOR)-internal tandem duplication (ITD) mutants, scientists employed mESCs expressing either transgenic or endogenous BCOR-ITD.

[Breast Cancer Research] Through whole-exome sequencing of 147 ductal carcinoma in situ (DCIS) cases, scientists characterized the genomic landscape of pure DCIS and identified genetic alterations associated with the risk of recurrence.

[Cell Death & Disease] Scientists found that DHX9 expression is frequently elevated in breast cancer (BC) cells and tissues, which suggests poor survival. The viability and motility of BC cells are irritated by enhanced DHX9 expression.

[Cancer Research] The authors generated orthotopic cell line–derived xenograft (CDX) models from breast cancer cell lines using both mammary intraductal injections and fat pad transplantations. The resulting mammary intraductal CDX and fat pad transplantation CDX models covered the full spectrum of disease progression.

[Expert Opinion on Therapeutic Targets] The authors review the metabolic strategies that HER2⁺ breast cancer cells adopt to enable trastuzumab resistance, grouping them into a structured classification that takes into account their functional nature, namely: (1) metabolic reprogramming; (2) adaptive stress response; and (3) metabolic-signaling crosstalk.

[Gut] The effects of peptidoglycan fragments on intestinal tumourigenesis were systematically evaluated using various murine models of colorectal cancer (CRC) and organoids derived from CRC patients.

[ACS Nano] Investigators developed a cochlear organoid culture system using synthetic GelMA hydrogel, which promoted the spontaneous clustering and organization of cochlear stem cells, thereby accelerating organoid formation and facilitating the functional maturation of cochlear hair cells.

[Cell Reports Medicine] Scientists used in vitro co-cultures and in vivo humanized mouse experimental models to examine autologous and allogeneic immune response to human primary cholangiocyte organoids as treatment for bile duct disorders.

[Science Advances] To understand the association of amyloid-β plaques and pathogenic infections in the central nervous system, scientists obtained viable short-interval postmortem human retinal tissue and generated human retinal organoids that contain electrophysiologically active neurons.