The effects of T-DPSC-conditioned media on human umbilical vein endothelial cell proliferation and migration were examined by MTT, wound healing, and trans-well migration assay.
[Stem Cell Research & Therapy]
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Category Archives: Endothelial Cell
Endothelial Cell NewsEndothelial Cell News is an online publication and email newsletter dedicated to the latest research studying the endothelium, blood vessels, and angiogenesis.
Specific research topics covered in Endothelial Cell News include the roles of endothelial cells in the regulation of physiological processes, as well as their implications in tumor progression, cardiovascular disease, endometriosis, wound healing, tissue regeneration and other diseases and disorders. We also provide updates on the latest job postings and upcoming events, helping connect everyone within the endothelial cell research field.
DPSCs Treated by TGF-β1 Regulate Angiogenic Sprouting of Three-Dimensionally Co-cultured HUVECs and DPSCs through VEGF-Ang-Tie2 Signaling
Syndecan-1 Promotes Angiogenesis in Triple-Negative Breast Cancer through the Prognostically Relevant Tissue Factor Pathway and Additional Angiogenic Routes
Scientists silenced Syndecan-1 in TNBC cell lines using a 3D human umbilical vein endothelial cell co-culture system to study its role in angiogenesis.
[Cancers]
Nassar, E., Hassan, N., El-Ghonaimy, E. A., Hassan, H., Abdullah, M. S., Rottke, T. V., Kiesel, L., Greve, B., Ibrahim, S. A., & Götte, M. (2021). Syndecan-1 Promotes Angiogenesis in Triple-Negative Breast Cancer through the Prognostically Relevant Tissue Factor Pathway and Additional Angiogenic Routes. Cancers, 13(10), 2318. https://doi.org/10.3390/cancers13102318 Cite
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Mitofusin-2 Stabilizes Adherens Junctions and Suppresses Endothelial Inflammation via Modulation of β-Catenin Signaling
Using three-dimensional structured illumination microscopy, scientists observed that the mitochondrial protein Mitofusin-2 (Mfn2) co-localized at the plasma membrane with VE-cadherin and β-catenin in endothelial cells during homeostasis.
[Nature Communications]
Kim, Y.-M., Krantz, S., Jambusaria, A., Toth, P. T., Moon, H.-G., Gunarathna, I., Park, G. Y., & Rehman, J. (2021). Mitofusin-2 stabilizes adherens junctions and suppresses endothelial inflammation via modulation of β-catenin signaling. Nature Communications, 12(1), 2736. https://doi.org/10.1038/s41467-021-23047-6 Cite
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Knockout RAGE Alleviates Cardiac Fibrosis through Repressing Endothelial-to-Mesenchymal Transition (EndMT) Mediated by Autophagy
Scientists found a decrease in endothelial cells and an increase in cells co-expressing CD31 and α-smooth muscle actin at eight weeks in heart tissue of mice subjected to transverse aortic constriction, which implied EndMT.
[Cell Death & Disease]
Zhang, L., He, J., Wang, J., Liu, J., Chen, Z., Deng, B., Wei, L., Wu, H., Liang, B., Li, H., Huang, Y., Lu, L., Yang, Z., Xian, S., & Wang, L. (2021). Knockout RAGE alleviates cardiac fibrosis through repressing endothelial-to-mesenchymal transition (EndMT) mediated by autophagy. Cell Death & Disease, 12(5), 1–13. https://doi.org/10.1038/s41419-021-03750-4 Cite
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A Junctional PACSIN2/EHD4/MICAL-L1 Complex Coordinates VE-Cadherin Trafficking for Endothelial Migration and Angiogenesis
Scientists showed that PACSIN2 recruited the trafficking regulators EHD4 and MICAL-L1 to the rear end of asymmetric adherens junctions to form a recycling endosome-like tubular structure.
[Nature Communications]
Malinova, T. S., Angulo-Urarte, A., Nüchel, J., Tauber, M., van der Stoel, M. M., Janssen, V., de Haan, A., Groenen, A. G., Tebbens, M., Graupera, M., Plomann, M., & Huveneers, S. (2021). A junctional PACSIN2/EHD4/MICAL-L1 complex coordinates VE-cadherin trafficking for endothelial migration and angiogenesis. Nature Communications, 12(1), 2610. https://doi.org/10.1038/s41467-021-22873-y Cite
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FUNDC1-Dependent Mitochondria-Associated Endoplasmic Reticulum Membranes Are Involved in Angiogenesis and Neoangiogenesis
Researchers determined the contributions of FUNDC1-mediated MAMs to angiogenesis in vitro and in vivo. In cultured endothelial cells, VEGF significantly increased the formation of MAMs and MAM-related proteins, including FUNDC1.
[Nature Communications]
Wang, C., Dai, X., Wu, S., Xu, W., Song, P., & Huang, K. (2021). FUNDC1-dependent mitochondria-associated endoplasmic reticulum membranes are involved in angiogenesis and neoangiogenesis. Nature Communications, 12(1), 2616. https://doi.org/10.1038/s41467-021-22771-3 Cite
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Targeting Inflammation in Atherosclerosis — from Experimental Insights to the Clinic
Scientists consider a gamut of attractive possibilities for modifying inflammation in atherosclerosis, including targeting pivotal inflammatory pathways such as the inflammasomes, inhibiting cytokines, manipulating adaptive immunity and promoting pro-resolution mechanisms.
[Nature Reviews Drug Discovery]
Soehnlein, O., & Libby, P. (2021). Targeting inflammation in atherosclerosis — from experimental insights to the clinic. Nature Reviews Drug Discovery, 1–22. https://doi.org/10.1038/s41573-021-00198-1 Cite
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Fabrication of Vascularized DPSC Constructs for Efficient Pulp Regeneration
Vascularized dental pulp stem cells (DPSC) constructs were fabricated by inducing endothelial differentiation, and then scientist investigated the behavior of differentiated DPSCs, the internal structure of cell constructs, and their pulp regenerative ability in vivo.
[Journal of Dental Research]
Katata, C., Sasaki, J. I., Li, A., Abe, G. L., Nör, J. E., Hayashi, M., & Imazato, S. (2021). Fabrication of Vascularized DPSC Constructs for Efficient Pulp Regeneration. Journal of Dental Research, 00220345211007427. https://doi.org/10.1177/00220345211007427 Cite
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Role of Epithelial-Endothelial Cell Interaction in the Pathogenesis of SARS-CoV-2 Infection
Scientists measured the susceptibility to infection, immune response, and expression of adhesion molecules, in human pulmonary microvascular endothelial cells exposed to conditioned medium from infected epithelial cells.
[Clinical Infectious Diseases]
Hui, K. P.-Y., Cheung, M.-C., Lai, K.-L., Ng, K.-C., Ho, J. C.-W., Peiris, M., Nicholls, J. M., & Chan, M. C.-W. (2021). Role of epithelial-endothelial cell interaction in the pathogenesis of SARS-CoV-2 infection. Clinical Infectious Diseases, ciab406. https://doi.org/10.1093/cid/ciab406 Cite
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Notch Regulates Vascular Collagen IV Basement Membrane through Modulation of Lysyl Hydroxylase 3 Trafficking
Researchers identified trafficking pathways that regulated collagen type IV (Col IV) deposition during angiogenic blood vessel development. They have identified the GTPase Rab10 as a major regulator of Col IV vesicular trafficking during vascular development using both in vitro imaging and biochemistry as well as in vivo models.
[Angiogenesis]
Gross, S. J., Webb, A. M., Peterlin, A. D., Durrant, J. R., Judson, R. J., Raza, Q., Kitajewski, J. K., & Kushner, E. J. (2021). Notch regulates vascular collagen IV basement membrane through modulation of lysyl hydroxylase 3 trafficking. Angiogenesis. https://doi.org/10.1007/s10456-021-09791-9 Cite
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HDAC Inhibitor, MS-275, Increases Vascular Permeability by Suppressing Robo4 Expression in Endothelial Cells
Researchers investigated the involvement of histone deacetylases (HDACs) in the regulation of Robo4 expression. An HDAC inhibitor, MS-275, which inhibits HDAC1, HDAC2, and HDAC3, was found to suppress Robo4 expression in endothelial cells.
[Tissue Barriers]
Kashio, T., Shirakura, K., Kinoshita, M., Morita, M., Ishiba, R., Muraoka, K., Kanbara, T., Tanaka, M., Funatsu, R., Hino, N., Koyama, S., Suzuki, R., Yoshioka, Y., Aoshi, T., Doi, T., & Okada, Y. (2021). HDAC inhibitor, MS-275, increases vascular permeability by suppressing Robo4 expression in endothelial cells. Tissue Barriers, 0(0), 1911195. https://doi.org/10.1080/21688370.2021.1911195 Cite
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