Accelerated Biosciences announced new data that further demonstrates statistically significant cytolysis with iPSc-derived natural killer cells programmed from its ethically sourced hTSCs.
[Accelerated Biosciences (BusinessWire, Inc.)]
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Scientists found that disruption of glyoxalase I , the gene encoding a major catabolic enzyme scavenging the carbonyl group, increased vulnerability to external carbonyl stress, leading to abnormal phenotypes in hiPSCs.
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Hara, T., Toyoshima, M., Hisano, Y., Balan, S., Iwayama, Y., Aono, H., Futamura, Y., Osada, H., Owada, Y., & Yoshikawa, T. (2021). Glyoxalase I disruption and external carbonyl stress impair mitochondrial function in human induced pluripotent stem cells and derived neurons. Translational Psychiatry, 11(1), 1–12. https://doi.org/10.1038/s41398-021-01392-w Cite
The KCNH2 was knocked out in the hESC H9 line using the CRISPR/Cas9 system. Using a chemically defined differentiation protocol, investigators obtained and verified human ether-a-go-go-related gene(hERG)-deficient cardiomyocytes. Subsequently, high-throughput microelectrode array assays and drug interventions were performed to characterise the electrophysiological signatures of hERG-deficient cell lines.
[Stem Cell Research & Therapy]
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Chang, Y., Li, Y., Bai, R., Wu, F., Ma, S., Saleem, A., Zhang, S., Jiang, Y., Dong, T., Guo, T., Hang, C., Lu, W., Jiang, H., & Lan, F. (2021). hERG-deficient human embryonic stem cell-derived cardiomyocytes for modelling QT prolongation. Stem Cell Research & Therapy, 12(1), 278. https://doi.org/10.1186/s13287-021-02346-1 Cite
The authors generated a patient-specific iPSC line from a family cohort carrying a hereditary atrial septal defect (ASD) mutation in GATA4 gene, as well as a hESC line carrying the isogenic T280M mutation using the CRISPR/Cas9 genome editing method. The GATA4-mutant iPSCs and ESCs were then differentiated into cardiomyocytes to model GATA4 mutation-associated ASD.
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Ye, L., Yu, Y., Zhao, Z.-A., Zhao, D., Ni, X., Wang, Y., Fang, X., Yu, M., Wang, Y., Tang, J.-M., Chen, Y., Shen, Z., Lei, W., & Hu, S. (2021). Patient-specific iPSC-derived cardiomyocytes reveal abnormal regulation of FGF16 in a familial atrial septal defect. Cardiovascular Research, cvab154. https://doi.org/10.1093/cvr/cvab154 Cite
To interrogate the C-mannosylome of hiPSCs, researchers compared the secretomes of CRISPR-Cas9 mutants lacking either the C-mannosyltransferase DPY19L1 or DPY19L3 to wild-type hiPSCs using mass spectrometry-based quantitative proteomics.
[Molecular & Cellular Proteomics]
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Cirksena, K., Hütte, H. J., Shcherbakova, A., Thumberger, T., Sakson, R., Weiss, S., Jensen, L. R., Friedrich, A., Todt, D., Kuss, A. W., Ruppert, T., Wittbrodt, J., Bakker, H., & Buettner, F. F. R. (2021). The C-mannosylome of human induced pluripotent stem cells implies a role for ADAMTS16 C-mannosylation in eye development. Molecular & Cellular Proteomics, 0(0). https://doi.org/10.1016/j.mcpro.2021.100092 Cite
Scientists showed that retinoic acid had a broader role in 2C‐like cells state, not only was one of the upstream regulators of the 2C‐like state in chemically defined media but also illuminated genetic and epigenetic regulations that govern ESCs to 2C‐like transition.
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Wang, Y., Na, Q., Li, X., Tee, W.-W., Wu, B., & Bao, S. (n.d.). Retinoic acid induces NELFA-mediated 2C-like state of mouse embryonic stem cells associates with epigenetic modifications and metabolic processes in chemically defined media. Cell Proliferation, n/a(n/a), e13049. https://doi.org/https://doi.org/10.1111/cpr.13049 Cite
The authors present the latest technical developments and discuss how bioengineering can further advance the optimization and external control of stem cell-based embryo-like structures.
[Stem Cell Reports]
RepliCel Life Sciences Inc. announced it has signed and launched a new collaborative research project agreement with the University of British Columbia (UBC) that enables the second stage of its cell marker research.
[RepliCel Life Sciences Inc.]
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MIMEDX Group, Inc. announced a collaborative agreement with the Wake Forest Institute for Regenerative Medicine to develop and advance scientific evidence in support of safe and effective clinical therapies. The partnership reflects a shared vision to advance regenerative science and innovative biologics that restore quality of life for patients, and is geared to further understanding product mechanisms of action and potential therapeutic targets.
[MIMEDX Group, Inc.]
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To investigate whether the autism spectrum disorder-associated CHD7 intronic variant affects neural development, researchers established human embryonic stem cells carrying this variant using CRISPR/Cas9 methods and found that the level of CHD7 mRNA significantly decreased compared to control.
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Scientists identified a homozygous stop-gain mutations in DSG2 that led to complete desmoglein-2 deficiency in a patient with severe biventricular heart failure. Induced pluripotent stem cells were generated from the patient, and the mutated DSG2 gene locus was heterozygously corrected to a normal allele via homology-directed repair.
[Human Molecular Genetics]
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Shiba, M., Higo, S., Kondo, T., Li, J., Liu, L., Ikeda, Y., Kohama, Y., Kameda, S., Tabata, T., Inoue, H., Nakamura, S., Takeda, M., Ito, E., Takashima, S., Miyagawa, S., Sawa, Y., Hikoso, S., & Sakata, Y. (2021). Phenotypic Recapitulation and Correction of Desmoglein-2-deficient Cardiomyopathy using Human Induced Pluripotent Stem Cell-derived Cardiomyocytes. Human Molecular Genetics, ddab127. https://doi.org/10.1093/hmg/ddab127 Cite