Investigators utilized an isogenic human induced pluripotent stem cell (hiPSC)-based system to demonstrate that conversion of APOE3 to APOE2 greatly reduced the production of amyloid-beta peptides in hiPSC-derived neural cultures.
The authors report a novel approach that produces, within ten days, the differentiation of human induced pluripotent stem cells into microglia by forced expression of both SPI1 and CEBPA.
[Stem Cell Reports]
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Researchers use mass spectrometry to describe the molecular events triggered by inhibition of Mek1/2 and Gsk3 and inhibition of Cdk8/19 on ESCs.
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Researchers reveal an intrinsic glutamatergic transmitter system directly modulating excitability and conductivity of atrial cardiomyocytes through controlling ionotropic glutamate receptor-gated currents.
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Xie, D., Xiong, K., Su, X., Wang, G., Ji, Q., Zou, Q., Wang, L., Liu, Y., Liang, D., Xue, J., Wang, L., Gao, X., Gu, X., Liu, H., He, X., Li, L., Yang, J., Lu, Y., Peng, L., & Chen, Y.-H. (2021). Identification of an endogenous glutamatergic transmitter system controlling excitability and conductivity of atrial cardiomyocytes. Cell Research, 1–14. https://doi.org/10.1038/s41422-021-00499-5 Cite
The authors evaluated the effect of ionizing radiation on chondrocyte-like cells differentiated from hiPSCs by determining their gene and microRNA expression profile and proteomic analysis.
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Stelcer, E., Kulcenty, K., Rucinski, M., Kruszyna-Mochalska, M., Skrobala, A., Sobecka, A., Jopek, K., & Suchorska, W. M. (2021). Ionizing radiation exposure of stem cell-derived chondrocytes affects their gene and microRNA expression profiles and cytokine production. Scientific Reports, 11(1), 7481. https://doi.org/10.1038/s41598-021-86230-1 Cite
Scientists proposed a novel mechanism for transcription factor EB (TFEB) governing pluripotency of mESCs by regulating the pluripotency transcriptional network.
[Cell Death & Disease]
Investigators developed two hiPSC/hESC-specific glycan-recognizing mouse antibodies, R-10G and R-17F, using the Tic hiPSC line as an antigen. R-10G recognized a low-sulfate keratan sulfate, and R-17F recognized lacto-N-fucopentaose-1.
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Scientists identified a novel role of PUMA in regulation of DNA repair in embryonic or iPSCs and immortalized hematopoietic progenitor cells after ionizing radiation.
[Signal Transduction and Targeted Therapy]
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Kang, J. W., Zhan, Z., Ji, G., Sang, Y., Zhou, D., Li, Y., Feng, H., & Cheng, T. (2021). PUMA facilitates EMI1-promoted cytoplasmic Rad51 ubiquitination and inhibits DNA repair in stem and progenitor cells. Signal Transduction and Targeted Therapy, 6(1), 1–11. https://doi.org/10.1038/s41392-021-00510-w Cite
Diamond Blackfan Anemia (DBA) iPSC lines were generated from blood mononuclear cells of DBA patients with inactivating mutations in RPS19 and subjected to hematopoietic differentiation to model disease phenotypes.
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Qanash, H., Li, Y., Smith, R. H., Linask, K., Young-Baird, S., Hakami, W., Keyvanfar, K., Choy, J. S., Zou, J., & Larochelle, A. (2021). Eltrombopag Improves Erythroid Differentiation in a Human Induced Pluripotent Stem Cell Model of Diamond Blackfan Anemia. Cells, 10(4), 734. https://doi.org/10.3390/cells10040734 Cite
Investigators established a hiPSC-derived neuron model for the investigation of axonal lysosome transport and maturation and showed that loss of JIP3 resulted in the accumulation of axonal lysosomes and the Alzheimer’s disease-related amyloid precursor protein-derived Aβ42 peptide.
[Molecular Biology of the Cell]
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Gowrishankar, S., Lyons, L., Rafiq, N. M., Roczniak-Ferguson, A., De Camilli, P., & Ferguson, S. M. (2021). Overlapping roles of JIP3 and JIP4 in promoting axonal transport of lysosomes in human iPSC-derived neurons. Molecular Biology of the Cell, mbc.E20-06-0382. https://doi.org/10.1091/mbc.E20-06-0382 Cite
To elucidate the underlying pathogenic mechanisms of myoclonus dystonia, scientists investigated iPSC-derived striatal medium spiny neurons from two myoclonus-dystonia patients carrying a heterozygous mutation in the SGCE gene in comparison to two matched healthy control lines.
[International Journal of Molecular Sciences]
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Kutschenko, A., Staege, S., Grütz, K., Glaß, H., Kalmbach, N., Gschwendtberger, T., Henkel, L. M., Heine, J., Grünewald, A., Hermann, A., Seibler, P., & Wegner, F. (2021). Functional and Molecular Properties of DYT-SGCE Myoclonus-Dystonia Patient-Derived Striatal Medium Spiny Neurons. International Journal of Molecular Sciences, 22(7), 3565. https://doi.org/10.3390/ijms22073565 Cite