ESC & iPSC News

ESC & iPSC News is an online resource dedicated to the latest research in the fields of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs).

Generation of Salivary Glands Derived from Pluripotent Stem Cells via Conditional Blastocyst Complementation

[Cell Reports] Scientists identified the Foxa2 lineage as a critical lineage for salivary gland development through conditional blastocyst complementation.

Optimizing In Vitro T Cell Differentiation by Using Induced Pluripotent Stem Cells with GFP-RUNX1 and McHerry-TCF7 Labeling

[Cell Proliferation] Investigators established a fluorescence reporter hiPSC line in which the endogenous expression of RUNX1 and TCF7 were illustrated by the GFP and mCherry fluorescence, respectively.

iPS Cell Generation-Associated Point Mutations Include Many C > T Substitutions via Different Cytosine Modification Mechanisms

[Nature Communications] Researchers found through WGS analysis of human and mouse iPSC lines that genomic mutations were de novo events and that, in addition to unmodified cytosine base prone to deamination, the DNA methylation sequence CpG represented a significant mutation-prone site.

The Totipotent 2C-Like State Safeguards Genomic Stability of Mouse Embryonic Stem Cells

[Journal of Cellular Physiology] The authors generated an inducible reporter cell system for specific elimination of two cell-like cells (2CLCs) from the ESC cultures to disrupt the equilibrium between ESCs and 2CLCs.

Tead4 and Tfap2c Generate Bipotency and a Bistable Switch in Totipotent Embryos to Promote Robust Lineage Diversification

[Nature Structural & Molecular Biology] The authors showed that TEAD4 and TFAP2C accelerated the loss of totipotency. These two factors paradoxically promoted and inhibited Hippo signaling before lineage diversification.

Molecular Mechanism of BMP Signal Control by Twisted Gastrulation

[Nature Communications] Investigators reported crystal structures of twisted gastrulation alone and in complex with a bone morphogenetic protein ligand, growth differentiation factor 5.

Parathyroid Gland Generation from Pluripotent Stem Cells

[Endocrinology and Metabolism] The author discusses current achievements and challenges in present and future parathyroid gland regenerative medicine.

Connecting Lab, Clinic, and Community

[Science] Communities Advancing Research Equity for Health, a new National Institutes of Health (NIH) research network in primary care settings, will expand beyond the existing capacity of clinical researchers to engage patients and communities that are currently not well represented in clinical research.

ASXLs Binding to the PHD2/3 Fingers of MLL4 Provides a Mechanism for the Recruitment of BAP1 to Active Enhancers

[Nature Communications] Researchers reported a novel function of the plant homeodomain (PHD) 2 and 3 fingers of mixed lineage leukemia 4 and 3 that bind to additional sexcombs like (ASXL) 2, a component of the polycomb repressive H2AK119 deubiquitinase complex.

Systematic Optimization of Culture Media for Maintenance of Human Induced Pluripotent Stem Cells Using the Response Surface Methodology

[Heliyon] The authors used the design of experiments and the response surface methodology, a powerful statistical tool, to generate empirical models for predicting the optimal culture conditions of the hiPSCs.

The Relationship between Nanoscale Genome Organization and Gene Expression in Mouse Embryonic Stem Cells during Pluripotency Transition

[Nucleic Acids Research] Using single-molecule microscopy approaches to reach nanoscale resolution, researchers showed that genome remodeling affected gene transcription during pluripotency transition.

Inheritance of H3K9 Methylation Regulates Genome Architecture in Drosophila Early Embryos

[EMBO Journal] Investigators showed that H3K9me3 was transmitted from the maternal germline to the next generation.

Since 2007, ESC & iPSC News has been providing updates on the top publications, reviews, and news in pluripotent stem cell research. Through our website, Twitter, and email newsletters, our editorial team is proud to help keep researchers and medical professionals up-to-date with the latest discoveries, jobs, and event postings in the pluripotent stem cell field.