An Induced Pluripotent Stem Cell Line (GZHMCi004-a) Derived from a Fetus with Heterozygous G380R Mutation in FGFR3 Gene Causing Achondroplasia

Scientists generated an induced pluripotent stem cell line GZHMCi004-A derived from umbilical cord blood mononuclear cells of a fetus with heterozygous G380R mutation in FGFR3 gene.
[Stem Cell Research]
Li, N., Lin, S. M., Li, Y., Sun, J., Zhang, L., & Chen, M. (2021). An induced pluripotent stem cell line (GZHMCi004-A) derived from a fetus with heterozygous G380R mutation in FGFR3 gene causing achondroplasia. Stem Cell Research, 53, 102322. https://doi.org/10.1016/j.scr.2021.102322 Cite
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Method for Selective Ablation of Undifferentiated Human Pluripotent Stem Cell Populations for Cell-Based Therapies

Scientists determined an appropriate in vitro doxorubicin dose that (a) eliminates residual undifferentiated stem cells before cell injection to prevent teratoma formation after cell transplantation and (b) does not cause cardiotoxicity in ESC-derived cardiomyocytes as demonstrated through contractility analysis, electrophysiology, topoisomerase activity assay, and quantification of reactive oxygen species generation.
[Experimental & Molecular Medicine]
Chour, T., Tian, L., Lau, E., Thomas, D., Itzhaki, I., Malak, O., Zhang, J. Z., Qin, X., Wardak, M., Liu, Y., Chandy, M., Black, K. E., Lam, M. P. Y., Neofytou, E., & Wu, J. C. (2021). Method for selective ablation of undifferentiated human pluripotent stem cell populations for cell-based therapies. JCI Insight, 6(7). https://doi.org/10.1172/jci.insight.142000 Cite
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Cytovia Therapeutics Files New Patents for its Natural Killer Cell Engager Multifunctional Antibodies (FLEX-NK®) for the Treatment of Hematological and Solid Tumors

Cytovia Therapeutics, Inc.announced that it has filed two provisional composition of matter patent applications for its FLEX-NK® platform, consisting of NKp46-based NK cell engager multifunctional antibodies targeting GPC3 for the treatment of solid tumors, including Hepatocellular carcinoma, and CD38 for the treatment of hematological malignancies, including Multiple Myeloma.
[Cytovia Therapeutics, Inc.]
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Establishment of Bovine Expanded Potential Stem Cells

Investigators explored the expanded potential stem cell (EPSC) technology for mammalian species refractory to the standard ESC approaches and report the successful establishment of bovine EPSCs from preimplantation embryos of both wild-type and somatic cell nuclear transfer.
[Proceedings of the National Academy of Sciences of the United States of America]
Zhao, L., Gao, X., Zheng, Y., Wang, Z., Zhao, G., Ren, J., Zhang, J., Wu, J., Wu, B., Chen, Y., Sun, W., Li, Y., Su, J., Ding, Y., Gao, Y., Liu, M., Bai, X., Sun, L., Cao, G., … Li, X. (2021). Establishment of bovine expanded potential stem cells. Proceedings of the National Academy of Sciences, 118(15). https://doi.org/10.1073/pnas.2018505118 Cite
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Centrosome‐Mediated Microtubule Remodeling During Axon Formation in Human iPSC‐Derived Neurons

The authors demonstrated that centrosomes played an essential role in controlling axon formation in hiPSC‐derived neurons.
[EMBO Journal]
Lindhout, F. W., Portegies, S., Kooistra, R., Herstel, L. J., Stucchi, R., Hummel, J. J. A., Scheefhals, N., Katrukha, E. A., Altelaar, M., MacGillavry, H. D., Wierenga, C. J., & Hoogenraad, C. C. (2021). Centrosome-mediated microtubule remodeling during axon formation in human iPSC-derived neurons. The EMBO Journal, n/a(n/a), e106798. https://doi.org/10.15252/embj.2020106798 Cite
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Fluid Shear Stress Promotes Embryonic Stem Cell Pluripotency via Interplay between β‐catenin and Vinculin in Bioreactor Culture

Researchers investigated how murine ESCs sense fluid shear stress and modulate a non‐canonical Wnt signaling response to promote pluripotency.
[Stem Cells]
Nath, S. C., Day, B., Harper, L., Yee, J., Hsu, C. Y.-M., Larijani, L., Rohani, L., Duan, N., Kallos, M. S., & Rancourt, D. E. (n.d.). Fluid Shear Stress Promotes Embryonic Stem Cell Pluripotency via Interplay between β-catenin and Vinculin in Bioreactor Culture. STEM CELLS, n/a(n/a). https://doi.org/https://doi.org/10.1002/stem.3382 Cite
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Gene and Protein Expression in Human Megakaryocytes Derived From Induced Pluripotent Stem Cells

iPSCs were reprogrammed from peripheral blood mononuclear cells and megakaryocytes were derived from the iPSCs in 194 healthy European American and African American subjects. mRNA was isolated and gene expression measured by RNA sequencing. Protein expression was measured in 62 of the subjects using mass spectrometry.
[Journal of Thrombosis and Haemostasis]
Gene and protein expression in human megakaryocytes derived from induced pluripotent stem cells - Kammers - - Journal of Thrombosis and Haemostasis - Wiley Online Library. (n.d.). Retrieved April 14, 2021, from https://onlinelibrary.wiley.com/doi/10.1111/jth.15334 Cite
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Defining Optimal Enzyme and Matrix Combination for Replating of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes at Different Levels of Maturity

The authors revealed the selection criteria of the dissociation approach and coating matrix for replating iPSC-cardiomyocytes based on the maturity and the requirements of further applications.
[Experimental Cell Research]
Koc, A., Goktas, S. S., Caglar, T. A., & Cagavi, E. (2021). Defining Optimal Enzyme and Matrix Combination for Replating of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes at Different Levels of Maturity. Experimental Cell Research, 112599. https://doi.org/10.1016/j.yexcr.2021.112599 Cite
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Stem Cell-Based Vascularization of Microphysiological Systems

The authors discuss key features and development of the native vasculature, linking that to how hiPSC-derived vascular cells have been generated, the state of the art in vascular microphysiological systems, and opportunities arising from interdisciplinary thinking.
[Stem Cell Reports]
Stem cell-based vascularization of microphysiological systems: Stem Cell Reports. (n.d.). Retrieved April 14, 2021, from https://www.cell.com/stem-cell-reports/fulltext/S2213-6711(21)00145-4?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS2213671121001454%3Fshowall%3Dtrue Cite
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APOE2 Mitigates Disease-Related Phenotypes in an Isogenic hiPSC-Based Model of Alzheimer’s Disease

Investigators utilized an isogenic human induced pluripotent stem cell (hiPSC)-based system to demonstrate that conversion of APOE3 to APOE2 greatly reduced the production of amyloid-beta peptides in hiPSC-derived neural cultures.
[Molecular Psychiatry]
Brookhouser, N., Raman, S., Frisch, C., Srinivasan, G., & Brafman, D. A. (2021). APOE2 mitigates disease-related phenotypes in an isogenic hiPSC-based model of Alzheimer’s disease. Molecular Psychiatry, 1–18. https://doi.org/10.1038/s41380-021-01076-3 Cite
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Efficient Conversion of Human Induced Pluripotent Stem Cells Into Microglia by Defined Transcription Factors

The authors report a novel approach that produces, within ten days, the differentiation of human induced pluripotent stem cells into microglia by forced expression of both SPI1 and CEBPA.
[Stem Cell Reports]
Chen, S.-W., Hung, Y.-S., Fuh, J.-L., Chen, N.-J., Chu, Y.-S., Chen, S.-C., Fann, M.-J., & Wong, Y.-H. (2021). Efficient conversion of human induced pluripotent stem cells into microglia by defined transcription factors. Stem Cell Reports, 0(0). https://doi.org/10.1016/j.stemcr.2021.03.010 Cite
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