Category Archives: ESC & iPSC

ESC & iPSC News is an online publication and email newsletter dedicated to the latest research in the fields of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs).
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Since 2007, ESC & iPSC News has been providing weekly updates on the top publications, reviews, and news in pluripotent stem cell research. Through our website, Twitter, and email newsletters, our editorial team is proud to help keep researchers and medical professionals up-to-date with the latest discoveries, jobs, and event postings in the pluripotent stem cell field.

Downregulation of E-cadherin in Pluripotent Stem Cells Triggers Partial EMT

Using a CRISPR interference system in human ESCs, scientists describe a molecular characterization of the effects of downregulation of E-cadherin, one of the main initiation events of EMT, as a unique start signal.
[Scientific Reports]
Aban, C. E., Lombardi, A., Neiman, G., Biani, M. C., La Greca, A., Waisman, A., Moro, L. N., Sevlever, G., Miriuka, S., & Luzzani, C. (2021). Downregulation of E-cadherin in pluripotent stem cells triggers partial EMT. Scientific Reports, 11(1), 2048. https://doi.org/10.1038/s41598-021-81735-1 Cite
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Greater Epithelial Ridge Cells Are the Principal Organoid-Forming Progenitors of the Mouse Cochlea

Scientists purified different cochlear cell types from neonatal mice, validated the composition of the different groups with single-cell RNA sequencing, and assessed the various groupsā€™ potential to grow into inner ear organoids.
[Cell Reports]
Kubota, M., Scheibinger, M., Jan, T. A., & Heller, S. (2021). Greater epithelial ridge cells are the principal organoid-forming progenitors of the mouse cochlea. Cell Reports, 34(3). https://doi.org/10.1016/j.celrep.2020.108646 Cite
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Cellular and Molecular Landscape of Mammalian Sinoatrial Node Revealed by Single-Cell RNA Sequencing

Deficiency of Vsnl1 not only reduced the beating rate of human iPSC- derived cardiomyocytes but also the heart rate of mice. Weighted gene co-expression network analysis unveiled the core gene regulation network governing the function of the sinoatrial node in mice.
[Nature Communications]
Liang, D., Xue, J., Geng, L., Zhou, L., Lv, B., Zeng, Q., Xiong, K., Zhou, H., Xie, D., Zhang, F., Liu, J., Liu, Y., Li, L., Yang, J., Xue, Z., & Chen, Y.-H. (2021). Cellular and molecular landscape of mammalian sinoatrial node revealed by single-cell RNA sequencing. Nature Communications, 12(1), 287. https://doi.org/10.1038/s41467-020-20448-x Cite
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Direct Reprogramming of Epithelial Cell Rests of Malassez into Mesenchymal-Like Cells by Epigenetic Agents

Epithelial cell rests of malassez was cultured in ESC medium with 1 ĀµM of 5-Azacytidine (5Aza), or 2 mM ofvalproic acid (Vpa), or a combination of 5Aza and Vpa. The cells stimulated with both 5Aza and Vpa were named as progenitor-dedifferentiated into stem-like cells.
[Scientific Reports]
Yoshida, K., Uehara, O., Kurashige, Y., Paudel, D., Onishi, A., Neopane, P., Hiraki, D., Morikawa, T., Harada, F., Takai, R., Sato, J., Saitoh, M., & Abiko, Y. (2021). Direct reprogramming of epithelial cell rests of malassez into mesenchymal-like cells by epigenetic agents. Scientific Reports, 11(1), 1852. https://doi.org/10.1038/s41598-020-79426-4 Cite
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Aberrant Development of Pancreatic Beta Cells Derived from Human iPSCs with FOXA2 Deficiency

Investigators generated iPSCs from a patient with FOXA2 haploinsufficiency followed by beta-cell differentiation to understand the role of FOXA2 during pancreatic beta-cell development.
[Cell Death & Disease]
Elsayed, A. K., Younis, I., Ali, G., Hussain, K., & Abdelalim, E. M. (2021). Aberrant development of pancreatic beta cells derived from human iPSCs with FOXA2 deficiency. Cell Death & Disease, 12(1), 1–17. https://doi.org/10.1038/s41419-021-03390-8 Cite
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OCT4 Induces Embryonic Pluripotency via STAT3 Signaling and Metabolic Mechanisms

Scientists used single-cell whole-genome transcriptional profiling and protein quantification to investigate the role of OCT4 in establishing pluripotency in the murine embryo.
[Proceedings of the National Academy of Sciences of the United States of America]
Stirparo, G. G., Kurowski, A., Yanagida, A., Bates, L. E., Strawbridge, S. E., Hladkou, S., Stuart, H. T., Boroviak, T. E., Silva, J. C. R., & Nichols, J. (2021). OCT4 induces embryonic pluripotency via STAT3 signaling and metabolic mechanisms. Proceedings of the National Academy of Sciences, 118(3). https://doi.org/10.1073/pnas.2008890118 Cite
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Pluripotent Stem Cells for Disease Modeling and Drug Discovery in Niemann-Pick Type C1

Scientists present a review focusing on studies that have used iPSCs for disease modeling and drug discovery in NPC1 and draw a comparison to commonly used NPC1 models.
[International Journal of Molecular Sciences]
Völkner, C., Liedtke, M., Hermann, A., & Frech, M. J. (2021). Pluripotent Stem Cells for Disease Modeling and Drug Discovery in Niemann-Pick Type C1. International Journal of Molecular Sciences, 22(2), 710. https://doi.org/10.3390/ijms22020710 Cite
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Generation and Manipulation of Human iPSC-Derived Platelets

The authors focus on the current status of the development, application and manipulation of iPSC-derived platelet-like particles, which has been leading the field of regenerative medicine using iPSC-derived blood cells and has reached the first-in-human clinical trial.
[Cellular and Molecular Life Sciences]
Sugimoto, N., & Eto, K. (2021). Generation and manipulation of human iPSC-derived platelets. Cellular and Molecular Life Sciences. https://doi.org/10.1007/s00018-020-03749-8 Cite
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PIAS1 Modulates Striatal Transcription, DNA Damage Repair, and SUMOylation with Relevance to Huntingtonā€™s Disease

Investigators evaluated the mechanistic contributions of protein inhibitor of activated STAT-1 (PIAS1) in Huntingtonā€™s disease (HD) mice and HD patient-derived induced pluripotent stem cells and found a link between PIAS1 and DNA damage repair pathways.
[Proceedings of the National Academy of Sciences of the United States of America]
Morozko, E. L., Smith-Geater, C., Monteys, A. M., Pradhan, S., Lim, R. G., Langfelder, P., Kachemov, M., Hill, A., Stocksdale, J. T., Cullis, P. R., Wu, J., Ochaba, J., Miramontes, R., Chakraborty, A., Hazra, T. K., Lau, A., St-cyr, S., Orellana, I., Kopan, L., … Thompson, L. M. (2021). PIAS1 modulates striatal transcription, DNA damage repair, and SUMOylation with relevance to Huntington’s disease. Proceedings of the National Academy of Sciences, 118(4). https://doi.org/10.1073/pnas.2021836118 Cite
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A Clinically Applicable and Scalable Method To Regenerate T-Cells from iPSCs for Off-the-Shelf T-Cell Immunotherapy

Scientists showed improved differentiation efficiency of T-cells from induced PSCs (iPSCs) derived from an antigen-specific cytotoxic T-cell clone, or from T-cell receptor-transduced iPSCs, as starting materials.
[Nature Communications]
Iriguchi, S., Yasui, Y., Kawai, Y., Arima, S., Kunitomo, M., Sato, T., Ueda, T., Minagawa, A., Mishima, Y., Yanagawa, N., Baba, Y., Miyake, Y., Nakayama, K., Takiguchi, M., Shinohara, T., Nakatsura, T., Yasukawa, M., Kassai, Y., Hayashi, A., & Kaneko, S. (2021). A clinically applicable and scalable method to regenerate T-cells from iPSCs for off-the-shelf T-cell immunotherapy. Nature Communications, 12(1), 430. https://doi.org/10.1038/s41467-020-20658-3 Cite
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Essential Roles of Insulin and IGF-1 Receptors during Embryonic Lineage Development

To directly define the roles of insulin and IGF-1 receptors in the maintenance of pluripotency and differentiation of stem cells, scientists knocked out both the receptors in induced pluripotent stem cells.
[Molecular Medicine]
Okawa, E. R., Gupta, M. K., Kahraman, S., Goli, P., Sakaguchi, M., Hu, J., Duan, K., Slipp, B., Lennerz, J. K., & Kulkarni, R. N. (2021). Essential roles of insulin and IGF-1 receptors during embryonic lineage development. Molecular Metabolism, 101164. https://doi.org/10.1016/j.molmet.2021.101164 Cite
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