Downregulation of E-cadherin in Pluripotent Stem Cells Triggers Partial EMT

Using a CRISPR interference system in human ESCs, scientists describe a molecular characterization of the effects of downregulation of E-cadherin, one of the main initiation events of EMT, as a unique start signal.
[Scientific Reports]
Aban, C. E., Lombardi, A., Neiman, G., Biani, M. C., La Greca, A., Waisman, A., Moro, L. N., Sevlever, G., Miriuka, S., & Luzzani, C. (2021). Downregulation of E-cadherin in pluripotent stem cells triggers partial EMT. Scientific Reports, 11(1), 2048. https://doi.org/10.1038/s41598-021-81735-1 Cite
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Greater Epithelial Ridge Cells Are the Principal Organoid-Forming Progenitors of the Mouse Cochlea

Scientists purified different cochlear cell types from neonatal mice, validated the composition of the different groups with single-cell RNA sequencing, and assessed the various groups’ potential to grow into inner ear organoids.
[Cell Reports]
Kubota, M., Scheibinger, M., Jan, T. A., & Heller, S. (2021). Greater epithelial ridge cells are the principal organoid-forming progenitors of the mouse cochlea. Cell Reports, 34(3). https://doi.org/10.1016/j.celrep.2020.108646 Cite
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Cellular and Molecular Landscape of Mammalian Sinoatrial Node Revealed by Single-Cell RNA Sequencing

Deficiency of Vsnl1 not only reduced the beating rate of human iPSC- derived cardiomyocytes but also the heart rate of mice. Weighted gene co-expression network analysis unveiled the core gene regulation network governing the function of the sinoatrial node in mice.
[Nature Communications]
Liang, D., Xue, J., Geng, L., Zhou, L., Lv, B., Zeng, Q., Xiong, K., Zhou, H., Xie, D., Zhang, F., Liu, J., Liu, Y., Li, L., Yang, J., Xue, Z., & Chen, Y.-H. (2021). Cellular and molecular landscape of mammalian sinoatrial node revealed by single-cell RNA sequencing. Nature Communications, 12(1), 287. https://doi.org/10.1038/s41467-020-20448-x Cite
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Direct Reprogramming of Epithelial Cell Rests of Malassez into Mesenchymal-Like Cells by Epigenetic Agents

Epithelial cell rests of malassez was cultured in ESC medium with 1 µM of 5-Azacytidine (5Aza), or 2 mM ofvalproic acid (Vpa), or a combination of 5Aza and Vpa. The cells stimulated with both 5Aza and Vpa were named as progenitor-dedifferentiated into stem-like cells.
[Scientific Reports]
Yoshida, K., Uehara, O., Kurashige, Y., Paudel, D., Onishi, A., Neopane, P., Hiraki, D., Morikawa, T., Harada, F., Takai, R., Sato, J., Saitoh, M., & Abiko, Y. (2021). Direct reprogramming of epithelial cell rests of malassez into mesenchymal-like cells by epigenetic agents. Scientific Reports, 11(1), 1852. https://doi.org/10.1038/s41598-020-79426-4 Cite
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Aberrant Development of Pancreatic Beta Cells Derived from Human iPSCs with FOXA2 Deficiency

Investigators generated iPSCs from a patient with FOXA2 haploinsufficiency followed by beta-cell differentiation to understand the role of FOXA2 during pancreatic beta-cell development.
[Cell Death & Disease]
Elsayed, A. K., Younis, I., Ali, G., Hussain, K., & Abdelalim, E. M. (2021). Aberrant development of pancreatic beta cells derived from human iPSCs with FOXA2 deficiency. Cell Death & Disease, 12(1), 1–17. https://doi.org/10.1038/s41419-021-03390-8 Cite
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OCT4 Induces Embryonic Pluripotency via STAT3 Signaling and Metabolic Mechanisms

Scientists used single-cell whole-genome transcriptional profiling and protein quantification to investigate the role of OCT4 in establishing pluripotency in the murine embryo.
[Proceedings of the National Academy of Sciences of the United States of America]
Stirparo, G. G., Kurowski, A., Yanagida, A., Bates, L. E., Strawbridge, S. E., Hladkou, S., Stuart, H. T., Boroviak, T. E., Silva, J. C. R., & Nichols, J. (2021). OCT4 induces embryonic pluripotency via STAT3 signaling and metabolic mechanisms. Proceedings of the National Academy of Sciences, 118(3). https://doi.org/10.1073/pnas.2008890118 Cite
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Pluripotent Stem Cells for Disease Modeling and Drug Discovery in Niemann-Pick Type C1

Scientists present a review focusing on studies that have used iPSCs for disease modeling and drug discovery in NPC1 and draw a comparison to commonly used NPC1 models.
[International Journal of Molecular Sciences]
Völkner, C., Liedtke, M., Hermann, A., & Frech, M. J. (2021). Pluripotent Stem Cells for Disease Modeling and Drug Discovery in Niemann-Pick Type C1. International Journal of Molecular Sciences, 22(2), 710. https://doi.org/10.3390/ijms22020710 Cite
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Generation and Manipulation of Human iPSC-Derived Platelets

The authors focus on the current status of the development, application and manipulation of iPSC-derived platelet-like particles, which has been leading the field of regenerative medicine using iPSC-derived blood cells and has reached the first-in-human clinical trial.
[Cellular and Molecular Life Sciences]
Sugimoto, N., & Eto, K. (2021). Generation and manipulation of human iPSC-derived platelets. Cellular and Molecular Life Sciences. https://doi.org/10.1007/s00018-020-03749-8 Cite
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PIAS1 Modulates Striatal Transcription, DNA Damage Repair, and SUMOylation with Relevance to Huntington’s Disease

Investigators evaluated the mechanistic contributions of protein inhibitor of activated STAT-1 (PIAS1) in Huntington’s disease (HD) mice and HD patient-derived induced pluripotent stem cells and found a link between PIAS1 and DNA damage repair pathways.
[Proceedings of the National Academy of Sciences of the United States of America]
Morozko, E. L., Smith-Geater, C., Monteys, A. M., Pradhan, S., Lim, R. G., Langfelder, P., Kachemov, M., Hill, A., Stocksdale, J. T., Cullis, P. R., Wu, J., Ochaba, J., Miramontes, R., Chakraborty, A., Hazra, T. K., Lau, A., St-cyr, S., Orellana, I., Kopan, L., … Thompson, L. M. (2021). PIAS1 modulates striatal transcription, DNA damage repair, and SUMOylation with relevance to Huntington’s disease. Proceedings of the National Academy of Sciences, 118(4). https://doi.org/10.1073/pnas.2021836118 Cite
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A Clinically Applicable and Scalable Method To Regenerate T-Cells from iPSCs for Off-the-Shelf T-Cell Immunotherapy

Scientists showed improved differentiation efficiency of T-cells from induced PSCs (iPSCs) derived from an antigen-specific cytotoxic T-cell clone, or from T-cell receptor-transduced iPSCs, as starting materials.
[Nature Communications]
Iriguchi, S., Yasui, Y., Kawai, Y., Arima, S., Kunitomo, M., Sato, T., Ueda, T., Minagawa, A., Mishima, Y., Yanagawa, N., Baba, Y., Miyake, Y., Nakayama, K., Takiguchi, M., Shinohara, T., Nakatsura, T., Yasukawa, M., Kassai, Y., Hayashi, A., & Kaneko, S. (2021). A clinically applicable and scalable method to regenerate T-cells from iPSCs for off-the-shelf T-cell immunotherapy. Nature Communications, 12(1), 430. https://doi.org/10.1038/s41467-020-20658-3 Cite
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Essential Roles of Insulin and IGF-1 Receptors during Embryonic Lineage Development

To directly define the roles of insulin and IGF-1 receptors in the maintenance of pluripotency and differentiation of stem cells, scientists knocked out both the receptors in induced pluripotent stem cells.
[Molecular Medicine]
Okawa, E. R., Gupta, M. K., Kahraman, S., Goli, P., Sakaguchi, M., Hu, J., Duan, K., Slipp, B., Lennerz, J. K., & Kulkarni, R. N. (2021). Essential roles of insulin and IGF-1 receptors during embryonic lineage development. Molecular Metabolism, 101164. https://doi.org/10.1016/j.molmet.2021.101164 Cite
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