ESC & iPSC News

ESC & iPSC News is an online resource dedicated to the latest research in the fields of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs).

Developmental Potency of Human ES Cell-Derived Mesenchymal Stem Cells Revealed in Mouse Embryos Following Blastocyst Injection

[Cell Reports] Scientists injected GFP-tagged human ESC-derived MSCs (EMSCs) into mouse blastocysts. EMSCs survived well and penetrated both the inner cell mass and trophectoderm, correlating to the higher anti-apoptotic capability of EMSCs than hESCs.

Microarray Analysis of lncRNAs and mRNAs in Spinal Cord Contusion Rats with iPSC-Derived A2B5+ Oligodendrocyte Precursor Cells Transplantation

[Heliyon] Induced pluripotent stem cell (iPSC)-derived A2B5+ oligodendrocyte precursor cells were obtained from mouse embryonic fibroblasts and injected into the lesion sites of spinal cord contusion rats.

Immune-Privileged Tissues Formed from Immunologically Cloaked Mouse Embryonic Stem Cells Survive Long Term in Allogeneic Hosts

[Nature Biomedical Engineering] Investigators showed that the overexpression of eight immunomodulatory transgenes in mouse ESCs was sufficient to immunologically ‘cloak’ the cells as well as tissues derived from them, allowing their survival for months in outbred and allogeneic inbred recipients.

Integration of Xeno-Free Single-Cell Cloning in CRISPR-Mediated DNA Editing of Human iPSCs Improves Homogeneity and Methodological Efficiency of Cellular Disease Modeling

[Stem Cell Reports] The authors provided a xeno-free method for single-cell cloning of CRISPRed iPSCs achieving a clonal survival of up to 70% within seven–ten days.

BACH1 Regulates the Differentiation of Vascular Smooth Muscle Cells from Human Embryonic Stem Cells via CARM1-Mediated Methylation of H3R17

[Cell Reports] Scientists foundnd that the loss of BACH1 in human embryonic stem cells (hESCs) attenuated the expression of vascular smooth muscle cell (VSMC) marker genes, whereas overexpression of BACH1 after mesoderm induction increased the expression of VSMC markers during in vitro hESC-VSMC differentiation.

γH2AX in Mouse Embryonic Stem Cells: Distribution During Differentiation and Following γ-Irradiation

[Cells & Development] Researchers established a model of mouse ESC differentiation and examined the dynamics of γH2AX foci during the process.

DOT1L Is a Barrier to Histone Acetylation During Reprogramming to Pluripotency

[Science Advances] The authors found that ESCs were depleted for H3K79me2 at shared locations of enrichment with somatic cells, which were highly and ubiquitously expressed housekeeping genes, and have lower RNA polymerase II at the transcription start site despite greater nascent transcription.

The Translational Oscillation in Oocyte and Early Embryo Development

[Nucleic Acids Research] Researchers uncovered a highly dynamic and oscillatory pattern of translational reprogramming that contributes to the translational regulation of specific mRNAs with different modes of polysomal occupancy/translation that are important for oocyte and embryo developmental competence.

Generation of Induced Pluripotent Stem Cell Lines IRMBi003-A and IRMBi003-B from a Healthy Donor to Model Alzheimer’s Disease

[Stem Cell Research] Investigators described the reprogramming of skin fibroblasts obtained from a healthy 59-year-old individual without Alzheimer’s disease.

Generation of an NCS1 Gene Knockout Human Induced Pluripotent Stem Cell Line Using CRISPR/Cas9

[Stem Cell Research] Researchers generated a NCS1 knockout human hiPSC line using CRISPR-Cas9 genome editing. It showed regular expression of pluripotent markers, normal iPSC morphology and karyotype as well as no detectable off-target effects on top 6 potentially affected genes.

UC San Diego Researchers Receive Close to $10M from California Stem Cell Research Agency

[UC San Diego] Two researchers at the University of California San Diego received close to $10 million in grants from the California Institute of Regenerative Medicine, the agency announced.

Overexpression of ZNF488 Supports Pancreatic Cancer Cell Proliferation and Tumorigenesis through Inhibition of Ferroptosis via Regulating SCD1-Mediated Unsaturated Fatty Acid Metabolism

[Current Stem Cell Research & Therapy] Human ESCcs were genetically modified to stably overexpress ferritin using the CRISPR-Cas9 system. Cellular toxicity associated with ferritin overexpression and manganese supplementation were assessed based on cell viability, proliferation, and metabolic activity.

Since 2007, ESC & iPSC News has been providing updates on the top publications, reviews, and news in pluripotent stem cell research. Through our website, Twitter, and email newsletters, our editorial team is proud to help keep researchers and medical professionals up-to-date with the latest discoveries, jobs, and event postings in the pluripotent stem cell field.