ESC & iPSC News

ESC & iPSC News is an online resource dedicated to the latest research in the fields of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs).

Mesodermal FGF and BMP Govern the Sequential Stages of Zebrafish Thyroid Specification

[Development] By analyzing Pax2a and Nkx2.4b expression dynamics in relation to endodermal FGF and BMP signaling activities in zebrafish embryos, researchers identified a Pax2a-expressing thyroid progenitor population that shows enhanced FGF signaling but lacks Nkx2.4b expression and BMP signaling.

Apicobasal RNA Asymmetries Regulate Cell Fate in the Early Mouse Embryo

[Nature Communications] Scientists uncovered apical-to-basal RNA asymmetries in outer blastomeres of 16-cell stage mouse preimplantation embryos. Basally directed RNA transport was facilitated in a microtubule- and lysosome-mediated manner.

GSK-3484862 Targets DNMT1 for Degradation in Cells

[NAR Cancer] Researchers showed that GSK-3484862 targets DNMT1 for protein degradation in both cancer cell lines and murine ESCs. DNMT1 depletion was rapid, taking effect within hours following GSK-3484862 treatment, leading to global hypomethylation.

Mouse Embryonic Stem Cell-Derived Blood–Brain Barrier Model: Applicability to Studying Antibody Triggered Receptor Mediated Transcytosis

[Fluids And Barriers Of The Cns] Investigators developed a mouse blood-brain barrier model, composed of mouse embryonic stem cell-derived brain endothelial-like cells, employing a directed monolayer differentiation strategy.

The Effect of Vitamin D3 and Valproic Acid on the Maturation of Human Induced Pluripotent Stem Cell-Derived Enterocyte-Like Cells

[Stem Cells] The authors identified a Notch activator and CYP modulator Valproic Acid, as a promotor for the maturation of iPSC-ent.

Differentiation and Single-Cell RNA-Seq Analyses of Human Pluripotent-Stem-Cell-Derived Renal Organoids

[Star Protocols] Investigators presented a protocol for the maintenance and differentiation of human pluripotent stem cells into renal organoids.

Propensity of Patient-Derived iPSCs for Retinal Differentiation: Implications for Autologous Cell Replacement

[Stem Cells Translational Medicine] IPSCs were generated from 15 patients aged 14-76 years old, differentiated into retinal organoids, and scored based on their retinal differentiation capacity. Despite significant differences in retinal differentiation propensity, RNA-sequencing revealed remarkable similarity between patient-derived iPSC lines prior to differentiation.

USP7 Represses Lineage Differentiation Genes in Mouse Embryonic Stem Cells by Both Catalytic and Noncatalytic Activities

[Science Advances] Researchers reported that USP7 maintains mESC identity through both catalytic activity–dependent and –independent repression of lineage differentiation genes. Usp7 depletion attenuated SOX2 levels and derepresses lineage differentiation genes thereby compromising mESC pluripotency.

RNA Stability Controlled by m6A Methylation Contributes to X-to-Autosome Dosage Compensation in Mammals

[Nature Structural & Molecular Biology] The authors showed that X-chromosomal transcripts have fewer m6A modifications and are more stable than their autosomal counterparts.

The Promises and Pitfalls of CRISPR-Mediated Base Editing in Stem Cells

[CRISPR Journal] Investigators discuss the strategies for achieving exact base edits in the creation of various stem cell-based models for use in elucidating disease mechanisms and assessing drug efficacy.

First Female Arab Astronaut Reaches ISS for Historic Mission to Conduct Stem Cell & Cancer Research

[The Free Press Journal] Rayyanah Barnawi, the first female Arab astronaut, has successfully arrived at the International Space Station as part of Axiom Space's second private mission.

Modulation of WNT, Activin/Nodal, and MAPK Signaling Pathways Increases Arterial Hemogenic Endothelium and Hematopoietic Stem/Progenitor Cell Formation during Human iPSC Differentiation

[Stem Cells] Researchers modulated WNT, Activin/Nodal, and MAPK signaling pathways by stage-specific addition of small molecule regulators CHIR99021, SB431542, and LY294002, respectively, and measured the impact on hematoendothelial formation in culture.

Since 2007, ESC & iPSC News has been providing updates on the top publications, reviews, and news in pluripotent stem cell research. Through our website, Twitter, and email newsletters, our editorial team is proud to help keep researchers and medical professionals up-to-date with the latest discoveries, jobs, and event postings in the pluripotent stem cell field.