ESC & iPSC News

ESC & iPSC News is an online resource dedicated to the latest research in the fields of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs).

Generation of Hypothalamic Neural Stem Cell-Like Cells In Vitro from Human Pluripotent Stem Cells

[Stem Cell Reports] Scientists differentiated retina and anterior neural fold homeobox(RAX)::VENUS knockin hESCs into hypothalamic organoids and sorted RAX+ cells from mature organoids. The isolated RAX+ cells formed neurospheres and exhibited self-renewal and multipotency.

Highly Efficient and Rapid Generation of Human Pluripotent Stem Cells by Chemical Reprogramming

[Cell Stem Cell] By screening for small molecule boosters and systematically optimizing the original condition, scientists established a robust, chemically defined reprogramming protocol, which greatly shortened the induction time and enabled highly reproducible and efficient generation of human somatic cells to PSCs.

Synthetic Regulatory Genomics Uncovers Enhancer Context Dependence at the Sox2 Locus

[Molecular Cell] Researchers analyzed the endogenous Sox2 locus using Big-IN to scarlessly integrate large DNA payloads incorporating deletions, rearrangements, and inversions affecting single or multiple DNase I hypersensitive sites, as well as surgical alterations to transcription factor recognition sequences.

Loss of H3K9 Trimethylation Alters Chromosome Compaction and Transcription Factor Retention during Mitosis

[Nature Structural & Molecular Biology] Investigators showed that enzymes that catalyze H3K9 methylation, such as Suv39h1, Suv39h2, G9a, and Glp, were also retained on mitotic chromosomes.

Induction of Fetal Meiotic Oocytes from Embryonic Stem Cells in Cynomolgus Monkeys

[EMBO Journal] The authors established a foundation for in vitro oogenesis in cynomolgus (cy) monkeys: cy female ESCs harboring one active and one inactive X chromosome differentiated robustly into primordial germ cell-like cells, which in xenogeneic reconstituted ovaries developed efficiently into oogonia and, further into meiotic oocytes at the zygotene stage.

Cell-Type Dependent Regulation of Pluripotency and Chromatin Remodeling Genes by Hydralazine

[Stem Cell Research & Therapy] Adult (aHF) and neonatal (nbHF) human fibroblasts were treated with hydralazine and/or valproic acid, and viability and gene expression assays for OCT4, NANOG, c-MYC, KLF4, DNMT1, TET3, ARID1A, and ARID2 by quantitative PCR were performed.

Long Noncoding RNA Lnc530 Localizes on R-Loops and Regulates R-Loop Formation and Genomic Stability in Mouse Embryonic Stem Cells

[Stem Cell Reports] Researchers reported that mESCs could efficiently prevent unscheduled R-loop formation, and a long noncoding RNA Lnc530 played a regulatory role.

Commercial Dishes with Gelatin-Free Microstructured Inserts for Elongated Stem Cell Self-Renewal and Pluripotency

[iScience] Scientists reported scalable fabrication of 2i-functionalized micro-pyramid-array inserts for use in commercial multiwell plates, as the alternative cultivation platform for maintaining long-term self-renewal and pluripotency of multiple mESCs and miPSCs.

Particle-Tracking-Based Strategy for the Optimization of Agitation Conditions in a Suspension Culture of Human Induced Pluripotent Stem Cells in a Shaking Vessel

[Journal of Bioscience and Bioengineering] Investigators observed that cell growth was ideally maintained by factors such as optimal acceleration, aggregate formation, and cell death.

Lymphoid Cell Development from Fetal Hematopoietic Progenitors and Human Pluripotent Stem Cells

[Immunological Reviews] The authors examine lymphoid development from non-hematopoietic stem cell progenitor cells and technological advances in the differentiation of human lymphoid cells from PSCs for clinical translation.

Stice, He Named UGA’s First Two Regents’ Entrepreneurs

[University of Georgia] The University System of Georgia Board of Regents approved two University of Georgia professors, Steven Stice and Biao He, as Regents’ Entrepreneurs.

Monash Awarded More than $53M through MRFF for Medical and Health Research

[Monash University] The Australian Government has awarded more than $53M through the Medical Research Future Fund (MRFF) to 21 Monash researchers for research into cardiovascular disease, respiratory disease, dementia, cancer, primary health care, nutrition, and more.

Since 2007, ESC & iPSC News has been providing updates on the top publications, reviews, and news in pluripotent stem cell research. Through our website, Twitter, and email newsletters, our editorial team is proud to help keep researchers and medical professionals up-to-date with the latest discoveries, jobs, and event postings in the pluripotent stem cell field.