LNCaP-AI Prostate Cancer Cell Line Establishment by Flutamide and Androgen-Free Environment to Promote Cell Adherent

The LNCaP-androgen dependent cell line was cultured and passaged 60 times over 16 months. The morphology of the LNCaP-androgen independent (AI) cell line was observed. Androgen receptor levels identification were detected in qRT-PCR and Western blot assay.