Endothelial Cell News 4.07 March 4, 2019 | |
| |
TOP STORYDKK3 (Dikkopf-3) Transdifferentiates Fibroblasts into Functional Endothelial Cells DKK3 overexpression in human fibroblasts under defined conditions for four days led to a notable change in cell morphology and progenitor gene expression. Further culture in endothelial cell (EC) defined media led to differentiation of these progenitors into functional ECs capable of angiogenesis both in vitro and in vivo, which was regulated by the VEGF /miR-125a-5p/signal transducer and activator of transcription factor 3 axis. [Arterioscler Thromb Vasc Biol] Abstract | |
| |
PUBLICATIONS(Ranked by impact factor of the journal)Scientists indicated that induced pluripotent stem cells-derived endothelial cells (iPSC-ECs) exposed to hyperglycemia had impaired autophagy, increased mitochondria fragmentation, and were associated with increased calpain activity. In addition, hyperglycemic iPSC-ECs had increased susceptibility to cell death when subjected to a secondary insult-simulated ischemia-reperfusion injury. [Stem Cell Reports] Full Article In vitro, 3-hydroxy-4,5-bis-benzyloxy-6-benzyloxymethyl-2-phenyl2-oxo-2λ5-[1,2]oxaphosphinane (PST 3.1a) inhibited all steps that regulate angiogenesis, including migration, proliferation, adhesion, and tube formation. Mechanistically, PST 3.1a altered interaction of VEGF receptor 2 and glycosylation-regulating protein galectin-1, a key component regulating angiogenesis associated with tumor resistance. [FASEB J] Abstract Tmem30a Deficiency in Endothelial Cells Impairs Cell Proliferation and Angiogenesis Scientists indicated that knockdown of TMEM30A in primary human retinal endothelial cells (ECs) led to reduced tube formation. In mice, EC-specific deletion of Tmem30a led to retarded retinal vascular development with a hyperpruned vascular network as well as blunted-end, aneurysm-like tip ECs with fewer filopodia at the vascular front and reduced number of tip cells. [J Cell Sci] Abstract Low Density Lipoprotein Mimics Insulin Action on Autophagy and Glucose Uptake in Endothelial Cells Researchers demonstrated that low density lipoprotein could suppress endothelial autophagy through activation of the PI3K/Akt/mTOR signaling pathway and could promote glucose uptake by translocating glucose transporter 1 from cytoplasm to the cell membrane, actions similar to those of insulin. [Sci Rep] Full Article To overcome the problematic properties of adnectin, a novel form of adnectin C was designed by its fusion to a biodegradable polymeric peptide containing Pro/Ala/Ser (PAS) repetitive residues. E. coli-expressed recombinant fused and unfused proteins were compared in terms of bioactivity, physicochemical, and pharmacokinetic properties using standard methods. [Sci Rep] Full Article After overexpression of growth-arrest specific transcript 5 (GAS5), cells showed increased proliferative activity, decreased cell apoptosis rate and apoptosis index, enhanced cell migration ability, increased number of lumen formation, increased mRNA expression of VEGF in cells and the secretion in the supernatant, decreased activity of reactive oxygen species (ROS) and superoxide dismutase in cells, and decreased content of ROS in cells. [J Cell Physiol] Abstract Investigators report that epidermal growth factor receptor (EGFR) ligands induced by the upregulation of VEGF-A and VEGF-C via the VEGF receptor 2/nuclear factor-κB signaling pathway in endothelial cells, may trigger EGFR signaling in bladder cancer cells and promote bladder cancer progression. [Int J Oncol] Full Article To improve complement regulation on human cells without interfering with antimicrobial activity, scientists identified an anti-factor H (FH) monoclonal antibody that induced increased FH-mediated protection of primary human endothelial cells from complement, while preserving the complement-mediated killing of bacteria. [Blood Adv] Abstract | Graphical Abstract Subscribe to one of our other 19 science newsletters such as Extracellular Matrix News & Cell Therapy News. | |
| |
REVIEWSThe authors describe the evidence that tumors located at numerous anatomical sites can exploit vessel co-option. They also discuss the proposed molecular mechanisms involved and the multifaceted implications of vessel co-option for patient outcomes. [Nat Rev Clin Oncol] Full Article Angiopoietin-1 and Angiopoietin-2 Inhibitors: Clinical Development Investigators discuss the current understanding of the Tie2-angiopoietin system and its role in tumor growth and metastasis. They also focus on preclinical and clinical data published to date that have evaluated Tie2-angiopoietin inhibition. [Curr Oncol Rep] Abstract Visit our reviews page to see a complete list of reviews in the endothelial cell research field. | |
| |
INDUSTRY NEWSOutlook Therapeutics Submits IND Application for ONS-5010 to the FDA Outlook Therapeutics, Inc. announced the submission of its Investigational New Drug (IND) application with the FDA for ONS-5010. ONS-5010 is an innovative monoclonal antibody therapeutic product candidate being developed for wet age related macular degeneration, diabetic macular edema and branch retinal vein occlusion. [Outlook Therapeutics, Inc.] Press Release Innovent Biologics, Inc. announced that the first patient has been dosed in ORIENT-32, a trial that evaluates Tyvyt®, a fully human anti-programmed cell death protein 1 (anti-PD-1) monoclonal antibody, in combination with IBI305, a recombinant humanized anti-VEGF monoclonal antibody, as first-line treatment for patients with advanced hepatocellular carcinoma. [Innovent Biologics, Inc.] Press Release | |
| |
POLICY NEWSPeer-Review Experiments Tracked in Online Repository A grass-roots group of biologists has started a website to keep track of the proliferation of experiments in academic peer review — including trials and platforms aimed at making the review process faster, cheaper, and more transparent and interactive. At launch, it listed around two dozen peer-review projects or trials, filed under categories such as transparency; quality of review; bias in review; speed; and incentives and recognition for reviewing. [Nature News] Editorial NIH Letters Asking about Undisclosed Foreign Ties Rattle US Universities The National Institutes of Health (NIH) has recently sent letters to dozens of major US research universities asking them to provide information about specific faculty members with NIH funding who are believed to have links to foreign governments that the Bethesda, Maryland–based institute did not know about. [ScienceInsider] Editorial University of California Breaks Up With Major Publisher Over Research Access Dispute After months of negotiations, the University of California system has decided not to renew its subscriptions to journals put out by the major publisher Elsevier — a decision that could have big ripple effects for the way that academic research gets read and paid for. [STAT News] Editorial Big Pharma Is Embracing Open-Access Publishing Like Never Before In a literature analysis, researchers found that the proportion of open-access papers published by 23 large drug companies, such as Pfizer and Roche, almost doubled between 2009 and 2016, and has overtaken the proportion of freely available papers published generally in medicine-related fields. [Nature News] Editorial
| |
EVENTSNEW Cell Symposia: Engineering Organoids and Organs Visit our events page to see a complete list of events in the community.
| |
JOB OPPORTUNITIESNEW Faculty Position – Bioengineering (University of Pittsburgh) NEW Postdoctoral Position – Endothelial Cell Protection (University of Bern) Postdoctoral Position – Angiogenesis/Vascular Biology (Johns Hopkins University) Vascular Biologist – RNA Therapeutics (Guide Therapeutics) Postdoctoral Fellow – Vascular Biology (Stanford School of Medicine) Postdoctoral Fellow – Vascular Biology (University of Nevada, Reno) Tier 2 Canada Research Chair – Cardiovascular/Vascular Health (University of Ottawa) Faculty Position – Clinical & Experimental Therapeutics (Augusta University) Postdoctoral Position – Cardiovascular Research (University of Hawaii) Postdoctoral Fellows – Vascular Immunology & Immunotherapy (Uppsala University) Assistant/Associate Adjunct Professor – Cardiovascular Medicine (UC Davis) Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.
| |
Have we missed an important article or publication in Endothelial Cell News? Click here to submit! Comments or suggestions? Submit your feedback here. | |
|