Chemical Ablation of Tumor-Initiating Human Pluripotent Stem Cells This protocol describes two efficient and robust ways to chemically eliminate the tumor-initiating human pluripotent stem cells from monolayer culture. [Nat Protoc] Abstract DDB2 Suppresses Tumorigenicity by Limiting the Cancer Stem Cell Population in Ovarian Cancer A novel role was identified for DNA damage binding protein 2 (DDB2) in the tumorigenesis of ovarian cancer cells and the prognosis of ovarian cancer patients. [Mol Cancer Res] Abstract NAC, Tiron and Trolox Impair Survival of Cell Cultures Containing Glioblastoma Tumorigenic Initiating Cells by Inhibition of Cell Cycle Progression Investigators aimed to determine the mechanisms involved in the response to antioxidants of a human cell culture containing glioblastoma tumorigenic initiating cells. [PLoS One] Full Article The Culture of Cancer Cell Lines as Tumorspheres Does Not Systematically Result in Cancer Stem Cell Enrichment Scientists attempted to generate tumorspheres from several murine and human cancer cell lines: B16-F10, HT-29, MCF-7 and MDA-MB-231 cells. Tumorspheres were obtained with variable efficiencies from all cell lines except from MDA-MB-231 cells. [PLoS One] Full Article Prognostic Value of Stem Cell Quantification in Stage II Colon Cancer Researchers aimed to evaluate the prognostic value of cancer stem cells in a highly homogeneous population of stage II colon cancer. One hundred stage II colon cancer patients treated by the same surgical team between 1977 and 2005 were retrospectively analyzed. [PLoS One] Full Article Hypoxia Promotes Stem-Like Properties of Laryngeal Cancer Cell Lines by Increasing the CD133+ Stem Cell Fraction Investigators cultured the two cell lines under hypoxia and normoxia and examined the influence of hypoxia on the expression of hypoxia-inducible factors and the cancer stem-like properties of these cells, including cell cycle distribution, expression of stem cell genes (OCT4, SOX2 and NANOG) and laryngeal cancer stem cell surface marker (CD133), proliferation, invasion, colony formation and sphere formation capacity. [Int J Oncol] Abstract Genistein-Inhibited Cancer Stem Cell-Like Properties and Reduced Chemoresistance of Gastric Cancer Scientists showed that low doses of genistein, extracted from Millettia nitida Benth var hirsutissima Z Wei, inhibit tumor cell self-renewal in two types of gastric cancer cells by colony formation assay and tumor sphere formation assay. [Int J Mol Sci] Abstract | Full Article Proliferation Inhibition and Differentiation Induction of Hepatic Cancer Stem Cells by Knockdown of BC047440: A Potential Therapeutic Target of Stem Cell Treatment for Hepatocellular Carcinoma The authors aimed to determine whether BC047440 is involved in maintaining hepatic cancer stem cells (HCSC) malignant behavior (including proliferation and differentiation). They demonstrated that BC047440 expression was markedly upregulated in HCSCs. [Oncol Rep] Abstract |