Cell Fate Potential of Human Pluripotent Stem Cells Is Encoded by Histone Modifications Here, researchers revealed specific cell surface markers that allow subfractionation of human embryonic stem cells expressing hallmark markers of pluripotency. [Cell Stem Cell] Indefinite Self-Renewal of ESCs through Myc/Max Transcriptional Complex-Independent Mechanisms Investigators addressed the question of how c-Myc helps preserve embryonic stem cell (ESC) status by performing loss-of-function studies with the Max gene, which encodes the best-characterized partner protein for all Myc family proteins. [Cell Stem Cell] Epigenetic Memory and Preferential Lineage-Specific Differentiation in Induced Pluripotent Stem Cells Derived from Human Pancreatic Islet Beta Cells Using two genetic lineage-tracing systems, scientists demonstrated the generation of induced pluripotent stem cell lines from human pancreatic islet beta cells. [Cell Stem Cell] Mammalian Hyperplastic Discs Homolog EDD Regulates miRNA-Mediated Gene Silencing Using a genetic screen in mouse embryonic stem (ES) cells, researchers identified mammalian hyperplastic discs protein EDD as a key component of the microRNA (miRNA) silencing pathway. [Mol Cell] Genes Inducing iPS Phenotype Play a Role in Hepatocyte Survival and Proliferation In Vitro and Liver Regeneration In Vivo Findings show that genes inducing iPS phenotype, even though expressed at lower levels than embryonic stem cells, they nonetheless are associated with control of apoptosis and cell proliferation in hepatocytes in culture and may play a role for such processes during liver regeneration. [Hepatology] Small-Molecule Inhibitors of the Wnt Pathway Potently Promote Cardiomyocytes from Human Embryonic Stem Cell–Derived Mesoderm The aim was to develop a human embryonic stem cell–based high-content screening assay to discover small molecules that drive cardiogenic differentiation after mesoderm is established to improve the understanding of the biology involved. [Circ Res] HIF Induces Human Embryonic Stem Cell Markers in Cancer Cells Scientists showed that hypoxia, through hypoxia-inducible factor (HIF), can induce a human embryonic stem cell-like transcriptional program, including the induced pluripotent stem cell inducers, OCT4, NANOG, SOX2, KLF4, cMYC, and microRNA-302 in 11 cancer cell lines. [Cancer Res] Electrospun Nanofibrillar Surfaces Promote Neuronal Differentiation and Function from Human Embryonic Stem Cells In this study, the impact of randomly oriented electrospun polyamide nanofibrous architecture on neurogenic differentiation of human embryonic stem cells compared to the lack of nanofibrous features in vitro in a neural inducing condition was examined. [Tissue Eng Part A] |