Extracellular Matrix News 8.01 January 12, 2017 | |
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TOP STORYStarved Epithelial Cells Uptake Extracellular Matrix for Survival Scientists demonstrated that under conditions of dietary restriction or growth factor starvation, where PI3K/mTOR signaling is decreased, matrix-attached human mammary epithelial cells upregulated and internalized β4-integrin along with its matrix substrate, laminin. [Nat Commun] Full Article | |
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PUBLICATIONS(Ranked by impact factor of the journal)Real-time PCR assay revealed that microRNA (miRNA)-483-5p was upregulated in articular cartilage from osteoarthritis (OA) patients and experimental OA mice induced by destabilization of the medial meniscus compared to their controls. Overexpression of miR-483-5p by intra-articular injection of lentivirus LV3-miR-483-5p significantly enhanced the severity of experimental OA. [Mol Ther] Abstract | Full Article A Modified Collagen Scaffold Facilitates Endogenous Neurogenesis for Acute Spinal Cord Injury Repair A linearly ordered collagen scaffold was used to prevent scar deposition, guide nerve regeneration and carry drugs to neutralize the inhibitory molecules. A collagen-binding EGFR antibody Fab fragment, CBD-Fab, was constructed to neutralize the myelin inhibitory molecules, which was demonstrated to promote neuronal differentiation and neurite outgrowth under myelin in vitro. [Acta Biomater] Abstract A Highly Versatile Adaptor Protein for the Tethering of Growth Factors to Gelatin-Based Biomaterials Scientists chose to investigate the capture of the basic fibroblast growth factor and the epidermal growth factor on a gelatin layer, as a model for the functionalization of collagen-based biomaterials. Their strategy relied on the use of two high affinity interactions, that is, the one between two distinct coil peptides as well as the one occurring between a collagen-binding domain and gelatin. [Acta Biomater] Abstract Lack of Collagen XV Is Protective after Ischemic Stroke in Mice Investigators report that collagen XV knock-out (ColXV KO) mice are resistant to experimental ischemic stroke. Interestingly, the infarcts of ColXV KO mice were as small as those of wild-type mice thrombolysed with recombinant tissue plasminogen activator, the actual treatment for ischemic stroke. [Cell Death Dis] Full Article The authors used quantitative proteomics to profile the composition of the extracellular matrix (ECM) of pancreatic islets in a mouse model of insulinoma characterized by a precisely timed angiogenic switch. Out of the 120 ECM proteins quantified, 35 were detected in significantly different abundance as pancreatic islets progressed from being hyperplastic to angiogenic to insulinomas. [Sci Rep] Full Article Researchers tested whether acellular mammary extracellular matrix (mECM) preparations are sufficient to direct differentiation of testicular-derived cells and embryonic stem cells to form functional mammary epithelial trees in vivo. They found that mECMs isolated from adult mice and rats were sufficient to redirect testicular derived cells to produce normal mammary epithelial trees within epithelial divested mouse mammary fat-pads. [Sci Rep] Full Article The authors investigated the effect of Src homology 2 domain-containing inositol 5′-phosphatase (SHIP) on extracellular matrix deposition in diabetic mice and high glucose-stimulated human renal tubular epithelial cells. The decreased SHIP and increased phospho-Akt were found in the renal tubular cells of diabetic mice, which were accompanied by over-expression of transforming growth factor-β1, α-smooth muscle actin, and secreted collagen type 3 and a low expression of E-cadherin compared to that in normal mice. [J Cell Biochem] Abstract Researchers extracted decellularized extracellular matrix from human umbilical vein endothelial cells (HUVECs-DECM) to provide a bioactive substratum conducive to the endothelial differentiation of dental stem cells. Compared to cells plated on tissue culture polystyrene, stem cells from exfoliated deciduous teeth cultured on the HUVECs-DECM demonstrated more regular arrangement and elongated morphology. [J Biomed Mater Res A] Abstract Using invasive MDA-MB 231 cells that endogenously produce membrane type-1 matrix metalloproteinase (MT1-MMP) and have naturally high pERK levels, investigators demonstrated the identical inverse relationship between MMP-2 and -9 transcript and protein levels, suggesting that this novel relationship is conserved amongst MT1-MMP positive breast cancer cells. [J Cell Commun Signal] Abstract Scientists investigated the role of NADPH oxidase (NOX) isoform NOX-2 in the production and degradation of extracellular matrix (ECM) by chondrocytes. Although interleukin-1β lowered the mRNA expression of type II collagen and aggrecan in mouse chondrocyte-like ATDC5 cells, RNA silencing of Nox2 did not change the mRNA expression of these major components of the ECM of cartilage. [Cell Tissue Res] Abstract | |
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REVIEWSThe Peculiar Functions of the Bacterial Extracellular Matrix The authors describe alternative functions of the extracellular matrix (ECM), highlighting how it benefits microbes beyond the biofilms. Next to protecting free-living cells, the ECM participates in signaling, migration, and genetic exchange either being freely shared with other species or being exclusive to siblings. [Trends Microbiol] Abstract Visit our reviews page to see a complete list of reviews in the extracellular matrix research field. | |
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INDUSTRY NEWSLifeNet Health has begun production of ViviGen® Cellular Bone Matrix in its Northwest headquarters in Renton, Washington. ViviGen, an innovative cellular allograft developed by LifeNet Health, incorporates viable bone cells that support the healing process. [LifeNet Health] Press Release Orthocell Announces Collaboration with Johnson & Johnson Innovation Regenerative medicine company Orthocell Limited announced a research collaboration agreement with DePuy Synthes Products, Inc., part of the Johnson & Johnson Family of Companies, for its Ortho-ATI® stem cell approach for the regeneration of degenerate tendons and ligaments. [Orthocell Limited] Press Release Children’s National and Inova Announce Three-Year, $2.8 Million Research and Education Collaboration Children’s National Health System and Inova this week announced a new three-year, $2.8 million research and education collaboration in maternal, fetal and neonatal medicine that aims to leverage the strengths of both health care facilities to improve the health of pregnant women and their children. [Children’s National Health System] Press Release | |
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POLICY NEWSWhite House Announces Review Process for Risky Virus Studies Federal officials released a plan to help U.S. agencies decide whether to fund controversial studies that make viruses more dangerous. The guidance may finally bring an end to a moratorium that has kept a handful of experiments funded by the Department of Health and Human Services on hold for more than two years. [ScienceInsider] Editorial US Energy Agency Strengthens Protections for Scientists The US Department of Energy has released new guidelines to protect researchers from political interference — a move that many say is long overdue. [Nature News] Editorial Cleveland Clinic Will Discipline Doctor Who Wrote Antivaccination Column The Cleveland Clinic released an apology from a staff physician who published an antivaccination column on the news website Cleveland.com. The doctor, Daniel Neides, will be “appropriately disciplined,” the Ohio hospital added in its own statement, which noted that the family physician’s views do not reflect his institution’s. [ScienceInsider] Editorial
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EVENTSNEW Keystone Symposium: Adaptations to Hypoxia in Physiology and Disease Visit our events page to see a complete list of events in the community.
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JOB OPPORTUNITIESNEW PhD Studentship – Light-Addressable Neuroactive Matrices (KU Leuven) Postdoctoral Scientist – Various Projects (Cedars-Sinai Medical Center) Postdoctoral Fellow & PhD Student – Novel Synthetic 3D Tissue Models (Thomas Jefferson University) Tenure Track Faculty Position – Kidney Bench Research (University of Pennsylvania) PhD Position – Microbial Biofilm Management (KU Leuven) Postdoctoral Fellow – Decorin and Perlecan (Thomas Jefferson University) Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.
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Home Extracellular Matrix News Volume 8.01 | Jan 12 2017