Down Syndrome-Associated Hematopoiesis Abnormalities Created by Chromosome Transfer and Genome Editing Technologies In vitro hematopoietic differentiation assays showed that the N-terminally truncated GATA1 (GATA1s) mutation blocked erythropoiesis irrespective of an extra chromosome 21, while trisomy 21 (Ts21) and the GATA1s mutation independently perturbed megakaryopoiesis and the combination of Ts21 and the GATA1s mutation synergistically contributed to an aberrant accumulation of skewed megakaryocytes. [Sci Rep] Full Article Expansion of Human and Murine Hematopoietic Stem and Progenitor Cells Ex Vivo without Genetic Modification Using MYC and Bcl-2 Fusion Proteins Researchers describe a novel approach to expand a cytokine-dependent hematopoietic stem and progenitor cell (HSPC) population ex vivo by culturing primary adult human or murine HSPCs with fusion proteins including the protein transduction domain of the HIV-1 transactivation protein and either MYC or Bcl-2. [PLoS One] Full Article Angiopoietin-Like Protein 3 Promotes Preservation of Stemness during Ex Vivo Expansion of Murine Hematopoietic Stem Cells To test the ability to expand hematopoietic stem cells in vitro prior to transplantation, two growth factor cocktails containing stem cell factor, thrombopoietin, fms-related tyrosine kinase-3 ligand or stem cell factor, thrombopoietin, insulin-like growth factor-2, fibroblast growth factor-1 either with or without the addition of angiopoietin-like protein-3 were used. [PLoS One] Full Article Resveratrol Increases the Bone Marrow Hematopoietic Stem and Progenitor Cell Capacity Scientists show that a three-week treatment of resveratrol increases the frequency and total numbers of normal bone marrow hematopoietic stem cells without any impact on their competitive repopulation capacity. [Am J Hematol] Abstract Ability of Circulating Human Hematopoietic Lineage Negative Cells to Support Hematopoiesis Investigators developed a novel mouse model and analyzed whether circulating human peripheral hematopoietic lineage negative/AP+ cells support hematopoiesis in vivo. [J Cell Biochem] Abstract CLINICAL RESEARCH Anti-CD22 90Y-Epratuzumab Tetraxetan Combined with Anti-CD20 Veltuzumab: A Phase I Study in Patients with Relapsed/Refractory, Aggressive Non-Hodgkin Lymphoma A lingering criticism of radioimmunotherapy in non-Hodgkin lymphoma is the use of cold anti-CD20 antibody along with the radiolabelled anti-CD20 antibody. Investigators instead combined radioimmunotherapy with immunotherapy targeting different B-cell antigens. [Haematologica] Abstract | Full Article Haploidentical Hematopoietic Stem Cell Transplantation in Adults with Philadelphia-Negative Acute Lymphoblastic Leukemia: No Difference in the High- and Low-Risk Groups Investigators evaluated the outcomes of haploidentical hematopoietic stem cell transplantation in adult Philadelphia chromosome-negative acute lymphoblastic leukemia in first complete remission patients. [Int J Cancer] Abstract Lower Dose of Antithymocyte Globulin (ATG) Does Not Increase Graft-vs-Host Disease (GvHD) in Patients Undergoing Reduced-Intensity Conditioning (RIC) Allogeneic Hematopoietic Stem Cell Transplant (allo HSCT) The appropriate dose of ATG been utilized in RIC allo HSCT is yet unknown. Researchers retrospectively compared patients who received 7.5 mg/kg (R-ATG) and 6 mg/kg (r-ATG). The cumulative incidences of acute GvHD (aGVHD) grade II-IV at 180 days were 46% and 41% and aGvHD grade III-IV were 11% and 18% in r-ATG and R-ATG, respectively. [Leuk Lymphoma] Abstract |