LABORATORY RESEARCH Carbonic Anhydrase IX from Cancer-Associated Fibroblasts Drives Epithelial-Mesenchymal Transition in Prostate Carcinoma Cells Investigators report that for aggressive prostate carcinoma, acknowledged to be reprogrammed toward an anabolic phenotype and to upload lactate to drive proliferation, extracellular acidification is mainly mediated by stromal cells engaged in a molecular cross-talk circuitry with cancer cells. [Cell Cycle] Abstract Impact of Tumor Vascularity on Responsiveness to Antiangiogenesis in a Prostate Cancer Stem Cell-Derived Tumor Model Researchers report that three angiogenesis inhibitors (axitinib, sorafenib, and DC101) all induced significantly greater decreases in tumor blood flow and microvessel density in PC3/2G7 tumors compared with PC3 tumors, as well as significantly greater decreases in tumor cell proliferation and cell viability and a greater increase in apoptosis. [Mol Cancer Ther] Abstract Epigenetic Modifications of Nrf2 by 3,3′-Diindolylmethane In Vitro in TRAMP C1 Cell Line and In Vivo TRAMP Prostate Tumors Researchers investigated the in vitro and in vivo epigenetics modulation of 3,3′-diindolylmethane (DIM) in TRAMP-C1 cells and in TRAMP mouse model. In vitro study utilizing TRAMP-C1 cells showed that DIM suppressed DNMT expression and reversed CpG methylation status of Nrf2 resulting in enhanced expression of Nrf2 and Nrf2-target gene NQO1. [AAPS J] Abstract OXER1, A G Protein-Coupled Oxoeicosatetraenoid Receptor, Mediates the Survival-Promoting Effects of Arachidonate 5-Lipoxygenase in Prostate Cancer Cells Researchers found that prostate cancer cells express high levels of 5-oxoeicosatetraenoic acid (5-oxoETE) receptor (OXER1), a G protein-coupled 5-oxoETE receptor, which delivers signals by generating diacyl-glycerol through phospholipase C-beta. [Cancer Lett] Abstract Evidence for a Pro-Proliferative Feedback Loop in Prostate Cancer: The Role of Epac1 and COX-2-Dependent Pathways Scientists examined the role of inflammatory mediators in Epac1-induced cellular proliferation by determining the expression of the pro-inflammatory markers p-cPLA2, COX-2, and PGE2 in prostate cancer cells treated with 8-CPT-2Me-cAMP. 8-CPT-2Me-cAMP treatment caused a 2-2.5-fold increase of p-cPLA2S505, COX-2, and PGE2 levels in human prostate cancer cell lines. [PLoS One] Full Article SATB1 Is Overexpressed in Metastatic Prostate Cancer and Promotes Prostate Cancer Cell Growth and Invasion 120 cases of prostatic carcinoma and 60 cases of benign prostate hyperplasia were analyzed for special AT-rich sequence binding protein 1 (SATB1) expression by immunohistochemistry. Researchers report that SATB1 staining was stronger in prostatic carcinomas with metastasis than in those without metastasis, but was absent in benign prostate hyperplasia. [J Transl Med] Abstract | Full Article Dishevelled-2 Silencing Reduces Androgen-Dependent Prostate Tumor Cell Proliferation and Migration and Expression of Wnt-3a and Matrix Metalloproteinases This study aimed to identify if dishevelled-2 (Dvl2) is a prostate cancer-associated gene and aimed to analyze the effects on the growth and invasive capacity of human prostate cancer cells. Silencing of Dvl2 mRNA expression significantly reduced LNCaP cell proliferation, motility, invasiveness and Wnt-3a, AR, matrix metalloproteinase (MMP)-2, and MMP-9 expression. [Mol Biol Rep] Abstract Prognostication of OCT4 Isoform Expression in Prostate Cancer The prognostic function of OCT4 isoforms in prostate cancer samples was examined by immunohistochemistry (IHC) and sensitivity and specificity of the antibodies used were evaluated by molecular biology techniques. IHC expression of OCT4 isoforms correlated with biochemical and pathological parameters, particularly biochemical recurrence-free survival. [Tumor Biol] Abstract CLINICAL RESEARCH ERG Overexpression and PTEN Status Predict Capsular Penetration in Prostate Carcinoma The authors examined the combined effect of two common genetic alterations, ERG and PTEN, in prostate carcinoma progression. This study presents the first evidence that ERG over expression and PTEN deletion is associated with greater risk of capsular penetration. [Prostate] Abstract A Phase II Trial of Dasatinib in Patients with Metastatic Castration-Resistant Prostate Cancer Treated Previously with Chemotherapy Investigators report that dasatinib induced a decrease in phytohemagglutinin-stimulated CSF2, CD40L, GZMB, and IL-2 mRNAs in blood cells, indicating target engagement. Decreases in plasma IL-6 and bone alkaline phosphatase, and in urinary N-telopeptide, were associated with disease control. [Anti-Cancer Drug] Abstract |