LABORATORY RESEARCH Exendin-4, a Glucagon-Like Peptide-1 Receptor Agonist, Attenuates Prostate Cancer Growth In in vitro experiments, Exendin-4 significantly decreased proliferation of the prostate cancer cell lines, LNCaP, PC3 and DU145, but not that of ALVA-41. This anti-proliferative effect depended on glucagon-like peptide-1 receptor expression. [Diabetes] Abstract Effects of Tumor-Suppressor Lysyl Oxidase Propeptide on Prostate Cancer Xenograft Growth and Its Direct Interactions with DNA Repair Pathways In vitro studies showed that recombinant lysyl oxidase propeptide (rLOX-PP) inhibits radiation-induced activating phosphorylations of ataxia-telangiectasia mutated and checkpoint kinase 2 and that exogenously added rLOX-PP protein can localize to the nucleus in both DU145 and PC3 cells. [Oncogene] Abstract EGFR Mediates Docetaxel Resistance in Human Castration-Resistant Prostate Cancer through the Akt-Dependent Expression of ABCB1 (MDR1) Investigators explored the mechanism of epidermal growth factor receptor (EGFR)-mediated docetaxel resistance in prostate cancer. [Arch Toxicol] Abstract DTX3L and ARTD9 Inhibit IRF1 Expression and Mediate in Cooperation with ARTD8 Survival and Proliferation of Metastatic Prostate Cancer Cells Researchers identified Deltex-3-like E3 ubiquitin ligase (DTX3L), diphtheria-toxin-like ADP-ribosyltransferase-9 (ARTD9) and ARTD8 as novel oncogenic survival factors in metastatic prostate cancer (mPCa) cells. Their studies revealed that DTX3L forms a complex with ARTD8 and mediates together with ARTD8 and ARTD9 proliferation, chemo-resistance and survival of mPCa cells. [Mol Cancer] Abstract | Full Article Identification of Beta-2 as a Key Cell Adhesion Molecule in PCa Cell Neurotropic Behavior: A Novel Ex Vivo and Biophysical Approach Researchers determined the effect of beta-2 expression on prostate cancer (PCa) cell neurotropic metastasis associated behavior. They overexpressed beta-2 as a fusion protein with enhanced cyan fluorescence protein in weakly aggressive LNCaP cells and observed neurotropic effects utilizing our novel ex vivo organotypic spinal cord co-culture model, and performed functional assays with neural matrices and atomic force microscopy. [PLoS One] Full Article Down-Regulation of mir-221 and mir-222 Restrain Prostate Cancer Cell Proliferation and Migration that Is Partly Mediated by Activation of SIRT1 The proliferation, apoptosis, cell cycle distinction, and migration capacity of prostate cells were determined following transfection of miR-221 or miR-222 inhibitor. The biological impact and regulation of silent information regulator 1 (SIRT1) on prostate cancer cells were investigated. [PLoS One] Full Article Identification of Genes Regulating Migration and Invasion Using a New Model of Metastatic Prostate Cancer Scientists used DU145 human prostate cancer cells and performed repeated rounds of orthotopic prostate injection and selection of subsequent lymph node metastases. Tumor growth, metastasis, cell migration and invasion were analyzed. [BMC Cancer] Full Article HOXB13 Regulates the Prostate-Derived Ets Factor: Implications for prostate Cancer Cell Invasion HOXB13 promoted PC3 cell migration and invasion. The DNA microarray analysis demonstrated that HOXB13 significantly suppressed the expression of the prostate-derived Ets factor. Accordingly, the expression of MMP-9 and survivin was regulated by HOXB13. [Int J Oncol] Abstract Efficient Delivery of Micro RNA to Bone-Metastatic Prostate Tumors by Using Aptamer-Conjugated Atelocollagen In Vitro and In Vivo The anticancer effect of nanoparticles in vivo was investigated using the survival times of human prostate cancer bone metastasis mice model. Luciferase assays of pGL-3 expression against PC3 and LNCaP cells showed that the transfection efficiency of the synthesized DNA/atelocollagen (ATE)-RNA aptamer complex was higher than that of the DNA/ATE complex. [Drug Deliv] Abstract CLINICAL RESEARCH Molecular Characterization of Enzalutamide-Treated Bone Metastatic Castration-Resistant Prostate Cancer Enzalutamide is a novel antiandrogen with proven efficacy in metastatic castration-resistant prostate cancer. Researchers evaluated enzalutamide’s effects on cancer and on androgens in blood and bone marrow, and associated these with clinical observations. [Eur Urol] Abstract Characterization of Desmoglein Expression in the Normal Prostatic Gland. Desmoglein 2 Is an Independent Prognostic Factor for Aggressive Prostate Cancer Scientists utilized immunofluorescence to examine Desmoglein 2 (DSG2) expression in normal prostate and in a clinically well-characterized cohort of prostate cancer patients. Correlation of DSG2 expression with clinico-pathological characteristics and biochemical recurrence was analyzed to assess its clinical significance. [PLoS One] Full Article |