Staphylococcus aureus Inhibits IL-8 Responses Induced by Pseudomonas aeruginosa in Airway Epithelial Cells Researchers examined the inflammatory responses to P. aeruginosa (PA) and S. aureus (SA) co-stimulations. Immortalized airway epithelial cells exposed to bacteria-free filtrates from PA induced a robust production of the neutrophil chemoattractant IL-8 while bacteria-free filtrates from SA had a minimal effect. [PLoS One] Full Article Glucocorticoid Dexamethasone Down-Regulates Basal and Vitamin D3 Induced Cathelicidin Expression in Human Monocytes and Bronchial Epithelial Cell Line Scientists showed that treatment with dexamethasone (Dex) inhibits basal mRNA expression of certain antimicrobial polypeptides in the THP-1 monocytic cell-line (THP-1 monocytes). Furthermore, pre-treatment with Dex inhibits vitamin D3 induced cathelicidin expression in THP-1 monocytes, primary monocytes and in the human bronchial epithelial cell line BCi NS 1.1. [Immunobiology] Full Article Evaluation of Uptake, Cytotoxicity and Inflammatory Effects in Respiratory Cells Exposed to Pristine and -OH and -COOH Functionalized Multi-Wall Carbon Nanotubes The authors evaluated on human alveolar epithelial cells and normal bronchial cells exposed to pristine-multi-wall carbon nanotubes (MWCNTs), MWCNTs-OH and MWCNTs-COOH: uptake by TEM, cell viability by different assays, membrane damage by the LDH assay and cytokine release by ELISA. [J Appl Toxicol] Abstract Paracoccidioides brasiliensis Induces Recruitment of α3 and α5 Integrins into Epithelial Cell Membrane Rafts, Leading to Cytokine Secretion Investigators demonstrated that P. brasiliensis yeasts promote IL-6 and IL-8 secretion by the human lung epithelial cell line A549 in an integrin-dependent manner. [Microbes Infect] Abstract LUNG CANCER PIWIL2 Promotes Progression of Non-Small Cell Lung Cancer by Inducing CDK2 and Cyclin A Expression PIWI genes expression was assayed in non-small cell lung cancer (NSCLC). To determine the effects of PIWIL2 on NSCLC cells, overexpression and interference assays were performed using the A549 and H460 cell lines. [J Transl Med] Full Article Synergistic Antiproliferative Effects of a New Cucurbitacin B Derivative and Chemotherapy Drugs on Lung Cancer Cell Line A549 The authors investigated the synergistic antiproliferative effects of a new semisynthetic derivative of cucurbitacin B with three chemotherapy drugs: cisplatin, irinotecan and paclitaxel on A549 cells. [Chem Res Toxicol] Abstract Norepinephrine Induced Epithelial–Mesenchymal Transition in HT-29 and A549 Cells In Vitro Investigators explored the effect of norepinephrine (NE) on cancer cell epithelial–mesenchymal transition and investigated the potential mechanism. HT-29 and A549 cells were treated with NE, β-adrenergic receptor antagonist (propranolol) or inhibitor of transforming growth factor-β receptor type I kinase. [J Cancer Res Clin Oncol] Abstract Non-Adherent Culture Induces Paclitaxel Resistance in H460 Lung Cancer Cells via ERK-Mediated Up-Regulation of βIVa-Tubulin Scientists explored whether chemoresistance is induced in epithelial cancer cells when cultured under non-adherent conditions. Increased paclitaxel-specific resistance was observed in floating cells compared to attached cells in H460, MCF-7, and HepG2 human cancer cell lines. [Biochem Biophys Res Commun] Full Article |