Dermal Cell News Volume 5.13 | Apr 15 2019

TOP STORY

Regeneration of a Bioengineered 3D Integumentary Organ System from iPS Cells

A three dimensional integumentary organ system (3D-IOS) comprises the skin and skin appendages such as hair follicles and sebaceous glands. Researchers describe how to induce the differentiation of murine induced pluripotent stem (iPS) cells into a 3D-IOS. [Nat Protoc] Abstract

(Ranked by impact factor of the journal)

DERMAL STEM CELLS & TISSUE REGENERATION

Intrinsic Planar Polarity Mechanisms Influence the Position-Dependent Regulation of Synapse Properties in Inner Hair Cells

The authors investigated whether signaling involved in planar polarity at the apical surface could influence position-dependent active zone properties at the inner hair cell (HC) base. Specifically, they tested the role of Gαi proteins and their binding partner LGN/Gpsm2 implicated in cytoskeleton polarization and HC orientation along the epithelial plane. [Proc Natl Acad Sci USA] Abstract

Therapeutic Potential of Patient iPSC-Derived iMelanocytes in Autologous Transplantation

Investigators generated human induced pluripotent stem cell (iPSC)-derived melanocytes from vitiligo patients using a three-dimensional system with enhanced differentiation efficiency, which showed characteristics of human epidermal melanocytes with high sequence similarity and involved in multiple vitiligo-associated signaling pathways. [Cell Rep] Full Article | Graphical Abstract

Small Molecule and Pooled CRISPR Screens Investigating IL17 Signaling Identify BRD2 as a Novel Contributor to Keratinocyte Inflammatory Responses

In RNA-Seq analysis, 438 interleukin-17A (IL17A)/tumor necrosis factor α dependent genes were reduced in bromodomain-containing protein 2 (BRD2)-deficient primary keratinocytes. Moreover, a number of genes important for keratinocyte homeostasis and cornification were dysregulated in BRD2-deficient keratinocytes. [ACS Chem Biol] Abstract | Graphical Abstract

Diversity of Dermal Fibroblasts as Major Determinant of Variability in Cell Reprogramming

Human dermal fibroblasts harvested from five different anatomical sites were cultured and their morphology, proliferation, apoptotic rate, ability to migrate, expression of mesenchymal or epithelial markers, differentiation potential and production of growth factors were evaluated in vitro. [J Cell Mol Med] Full Article

MicroRNA-155 Inhibition Restores Fibroblast Growth Factor 7 Expression in Diabetic Skin and Decreases Wound Inflammation

In vitro inhibition of miR-155 increased human keratinocyte scratch closure and topical inhibition of miR-155 in vivo in wounds increased murine fibroblast growth factor 7 protein expression and significantly enhanced diabetic wound healing. [Sci Rep] Full Article

USP15 Maintains Transforming Growth Factor-β Pathway Activity by Deubiquitinating TGF-β Receptor I (TBR1) during Wound Healing

Inhibition of cell proliferation and migration was observed in ubiquitin-specific peptidase 15 (USP15)-silenced human dermal fibroblasts. Through RNA sequencing, it was revealed that the transforming growth factor-β (TGB-β) pathway was suppressed after USP15 knockdown. [Am J Pathol] Abstract

SKIN CANCERS & DISORDERS

Transcriptional Regulation of Autophagy-Lysosomal Function in BRAF-Driven Melanoma Progression and Chemoresistance

Scientists showed that in BRAF^V600E-melanoma, autophagy is induced by the BRAF inhibitor, as part of a transcriptional program coordinating lysosome biogenesis/function, mediated by the TFEB transcription factor. [Nat Commun] Full Article

Molecular Targets for Tivantinib (ARQ 197) and Vasculogenic Mimicry in Human Melanoma Cells

Roundabout guidance receptor 4, a receptor protein implicated in axonal guidance and angiogenesis and its ligand Slit2 were expressed in human C8161 and WM793 melanoma cells, but absent in other melanoma cells including normal melanocytes. [Eur J Pharmacol] Abstract

Knockdown of Delta-Like 3 Restricts Lipopolysaccharide-Induced Inflammation, Migration and Invasion of A2058 Melanoma Cells via Blocking Twist1-Mediated Epithelial-Mesenchymal Transition

Melanoma A2058 cells were stimulated with lipopolysaccharide, with or without transfection of DLL3 siRNA, or DLL3 overexpression vector, or Twist1 siRNA. Cell migration and invasion were detected by wound healing and transwell invasion assay. [Life Sci] Abstract

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Cell-State Dynamics and Therapeutic Resistance in Melanoma from the Perspective of MITF and IFNγ Pathways

The authors focus on two different pivotal signaling pathways to construct the evolving trajectories of melanoma and describe each of the cell states. [Nat Rev Clin Oncol] Abstract

Mechanisms of Hair Cell Damage and Repair

Investigators discuss how several key hair cell structures can be damaged, and what is known about how they are repaired. [Trends Neurosci] Abstract

Visit our reviews page to see a complete list of reviews in the dermal cell research field.

Fibrocell Announces Collaboration with Castle Creek Pharmaceuticals to Develop and Commercialize FCX-007 Gene Therapy

Fibrocell Science, Inc. announced the completion of a collaboration agreement with Castle Creek Pharmaceuticals to develop and commercialize Fibrocell’s lead gene therapy candidate, FCX-007, for the treatment of recessive dystrophic epidermolysis bullosa. [Fibrocell Science, Inc.] Press Release

Mallinckrodt Completes Full Enrollment of Its Phase III Clinical Trial for StrataGraft^® Regenerative Skin Tissue

Mallinckrodt plc announced that Stratatech, a Mallinckrodt company, has completed patient enrollment in its ongoing pivotal Phase III clinical trial of StrataGraft^®, an investigational regenerative skin tissue. [Mallinckrodt plc] Press Release

Indonesian Mega-Science Agency in the Balance as Election Begins

Indonesians will vote in a national election that pits two old rivals against each other. The stakes are high for science. President Joko Widodo says that if he is re-elected, he will overhaul how much of the country’s research is organized and funded. The main opposition candidate has been silent on science so far. [Nature News] Editorial

African Research Projects Are Failing Because Funding Agencies Can’t Match Donor Money

Although African countries appreciate research grants from donor countries, they often chafe at the condition that they bring in their own money in order to be eligible. Some research projects fall by the wayside because African granting agencies simply have no way to provide their share of the money, sometimes called counterfunding, the heads of 15 national science councils in Africa said. [ScienceInsider] Editorial

NEW 2^nd Arab African International Cancer Congress (AAICC)
October 10-11, 2019
Cairo, Egypt

Visit our events page to see a complete list of events in the community.

NEW Postdoctoral Fellowship – Cancer Biology (University of Miami)

Research Assistant II – Melanoma Medical Oncology (MD Anderson Cancer Center)

Postdoctoral Fellowship – Epidermal Stem Cell Research (UC San Diego)

Postdoctoral Researcher – Immune-Epidermal Cell Stress Response (Queen Mary University of London)

Postdoctoral Fellowship – Immune Cell-Mediated Regulation (Hospital for Special Surgery)

Junior Faculty and Postdoctoral Positions – Epigenetic Regulation (Henry Ford Health System)

Postdoctoral Researcher – Skin Biology (University of Wisconsin-Madison)

Director – Arthritis and Musculoskeletal and Skin Diseases (National Institutes of Health)

Research Assistant – Burn Research (Sunnybrook Health Sciences Centre)

Postdoctoral Fellowship – Tumor Immunology and Cancer Metabolism (Roswell Park Comprehensive Cancer Center)

PhD Position – Epidermal Tissue Mechanics (Forschungszentrum Jülich)

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