Precise Detection of CRISPR/Cas9 Editing in Hair Cells in the Treatment of Autosomal Dominant Hearing Loss

In Atoh1-GFP;Kcnq4+/G229D mice, recapitulating a novel mutation the authors identified in a hereditary hearing loss pedigree, the high-efficiency editing of CRISPR/Cas9 in hair cells was precisely detected by sorting out labeled cells compared with only 1.45% efficiency in the whole cochlear tissue.