Integrated Flow Cytometry and Sequencing to Reconstruct Evolutionary Patterns from Dysplasia to Acute Myeloid Leukemia

Researchers hypothesized that the mutational status of bone marrow dysplastic cells and leukemic blasts, analyzed at the onset of AML using integrated multidimensional flow cytometry immunophenotyping and fluorescence-activated cell sorting with next-generation sequencing, could reconstruct leukemogenesis.