Immunofluorescence was used for antigen localization. After targeted antigen purification by electrophoresis and immunoblot, the antigen was identified by LC-MALDI-TOF/TOF mass spectrometry, immunofluorescence, and immunoprecipitation.
[Stem Cell Research & Therapy]
Shu, X., Cao, K.-Y., Liu, H.-Q., Yu, L., Sun, L.-X., Yang, Z.-H., Wu, C.-A., & Ran, Y.-L. (2021). Alpha-enolase (ENO1), identified as an antigen to monoclonal antibody 12C7, promotes the self-renewal and malignant phenotype of lung cancer stem cells by AMPK/mTOR pathway. Stem Cell Research & Therapy, 12(1), 119. https://doi.org/10.1186/s13287-021-02160-9 Cite
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