Concentration of Na⁺-Taurocholate-Cotransporting Polypeptide Expressed after In Vitro-Transcribed mRNA Transfection Determines Susceptibility of Hepatoma Cells for Hepatitis B Virus

Investigators utilized in vitro transcribed mRNA to introduce Na+-taurocholate-cotransporting polypeptide (NTCP) into cells. After transfection of mRNA, surface expression and functionality of NTCP was demonstrated by staining with an N-terminal HBV-preS peptide and bile acid uptake.