Intestinal Cell News 4.24 June 29, 2018 | |
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TOP STORYParasitic Helminths Induce Fetal-Like Reversion in the Intestinal Stem Cell Niche The authors examined infection by Heligmosomoides polygyrus, a co-evolved pathosymbiont of mice, to assess the epithelial response to disruption of the mucosal barrier. [Nature] Abstract | |
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PUBLICATIONS(Ranked by impact factor of the journal)Scientists demonstrated that YAP is a nucleocytoplasmic shuttling protein and its nuclear export is controlled by SET1A-mediated mono-methylation of YAP at K342, which disrupts the binding of YAP to CRM1. YAP mimetic methylation knockin mice were more susceptible to colorectal tumorigenesis. [Cancer Cell] Abstract | Graphical Abstract Scientists investigated the effects of Y-27632, an inhibitor of ROCK1 and ROCK2, on L-cell differentiation. Incubation of intestinal organoids with Y-27632 increased numbers of L cells and secretion of GLP1. [Gastroenterology] Abstract Integrative Multi‐Omics Analysis of Intestinal Organoid Differentiation The authors applied a multi‐omics framework on stem cell‐enriched and stem cell‐depleted mouse intestinal organoids to obtain a holistic view of the molecular mechanisms that drive differential gene expression during adult intestinal stem cell differentiation. [Mol Syst Biol] Abstract | Graphical Abstract Deficiency in Intestinal Epithelial GlcNAcylation Predisposes to Gut Inflammation Investigators found that levels of protein O‐GlcNAcylation and the expression of O‐GlcNAc transferase, the enzyme adding the O‐GlcNAc moiety, were reduced in intestinal epithelial cells in human IBD patients. [EMBO Mol Med] Abstract | Graphical Abstract Researchers developed a planar crypt array that replicated the spatial segregation and physiologic responses of primary mouse intestinal epithelial cells in the large intestine. [Lab Chip] Abstract | Graphical Abstract MicroRNA‐383 Acts as a Tumor Suppressor in Colorectal Cancer by Modulating CREPT/RPRD1B Expression The observed that cell-cycle‐related and expression‐elevated protein in tumor (CREPT) was up‐regulated but the expression of miR‐383 was down regulated in both colon cancer cell lines and colon tumor tissues. [Mol Carcinog] Abstract xMD-miRNA-seq to Generate Near In Vivo miRNA Expression Estimates in Colon Epithelial Cells Investigators isolated colon epithelial cells from two specimens and performed low-input small RNA-seq. They generated up to 600,000 miRNA reads from the samples. Isolated epithelial cells, had abundant epithelial-enriched miRNA expression and overall similar miRNA expression patterns to other epithelial cell populations. [Sci Rep] Full Article Researchers investigated which gut commensal bacteria were able to regulate the TGFB1 human promoter in intestinal epithelial cells using supernatants from cultured bacteria. They reported that Firmicutes and Fusobacteria supernatants were the most potent TGFB1 modulators in HT-29 cells. [Sci Rep] Full Article Proteomic Profiling of Enteroid Cultures Skewed towards Development of Specific Epithelial Lineages The authors performed label‐free quantitative proteomics to determine whether skewing murine enteroid cultures towards the goblet or paneth cell lineages results in changes in abundance of proteins associated with these cell lineages in vivo. [Proteomics] Abstract Necrotizing enterocolitis (NEC) rats showed decreased proliferating cell nuclear antigen expression, increased TUNEL-positive cells, higher expression of TNF-α, p-ERK1/2, and autophagy-related proteins in rat small intestine compared with their controls. Additionally, the LPS-stimulated IEC-6/NEC cells showed a significantly decreased proliferation and increased apoptosis compared with the control cells. [Cell Cycle] Abstract Modeling Cryptosporidium Infection in Human Small Intestinal and Lung Organoids The authors showed that Cryptosporidium can infect epithelial organoids derived from human small intestine and lung. The parasite propagates within the organoids and completes its complex life cycle. [Nat Microbiol] Abstract | Press Release Subscribe to one of our other 19 science newsletters such as Pulmonary Cell News & ESC & iPSC News. | |
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REVIEWSiPSC-Derived Enterocyte-Like Cells for Drug Absorption and Metabolism Studies The authors describe mechanisms underlying the differentiation of induced pluripotent stem cells (iPSCs) into enterocyte-like cells, appraise the usefulness of these cells in tridimensional intestinal models, and discuss their suitability to be used in the future for drug screening. [Trends Mol Med] Abstract Visit our reviews page to see a complete list of reviews in the intestinal cell research field. | |
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INDUSTRY NEWSInnovate Biopharmaceuticals Inc. announced it has agreed to collaborate with Professor O. Colin Stine, Ph.D., at the University of Maryland School of Medicine, whose research is focused on the study of molecular mechanism of bacterial invasion of the intestinal barrier and disruption of the microbiota in several infectious diseases. [Innovate Biopharmaceuticals Inc.] Press Release NOXXON Pharma N.V. announced that patient recruitment is almost complete in its ongoing clinical trial testing NOX-A12 alone and subsequently in combination with Merck & Co./MSD’s Keytruda® in metastatic, microsatellite stable pancreatic and colorectal cancer patients. [NOXXON Pharma N.V.] Press Release Pluristem Therapeutics Inc. reported positive data from studies conducted in collaboration with Fukushima Medical University evaluating PLX-R18 cells as a treatment for radiation damage to the gastrointestinal tract and bone marrow. [Pluristem Therapeutics Inc.] Press Release | |
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POLICY NEWSU.S. Lawmakers Want NIH and CDC Foundations to Say More About Donors A key congressional spending panel has fired a shot across the bow of two federally chartered medical foundations, warning that the way they disclose information about donors may not pass muster. [ScienceInsider] Editorial Science Candidates: High-Tech Smarts Aren’t Enough for Defeated Obama Aide For many first-time congressional candidates with science and technology backgrounds, fundraising can be a major obstacle. Not to Brian Forde, who was once a senior technology adviser to former-President Barack Obama. [ScienceInsider] Editorial
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EVENTSNEW 2018 National Cancer Research Institute (NCRI) Cancer Conference Visit our events page to see a complete list of events in the community.
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JOB OPPORTUNITIESNEW Assistant Professor – Cancer Biology (Université de Genève) NEW Postdoctoral Research Position – Cancer Biology (Medical Research Council) Postdoctoral Fellow – Colorectal Cancer (Dalhousie University) PhD position – Inflammatory Bowel Disease (Universitätsmedizin Berlin) Postdoctoral Fellow Position – Intestinal Research (Vanderbilt University Medical Center) Postdoctoral Position – Cancer Biology and Metastasis (MD Anderson Cancer Center) Associate/Full Researcher – Cancer Biology (University of Hawaii Cancer Center) Postdoctoral Fellowship – Inflammatory Bowel Disease (European Molecular Biology Laboratory) Postdoctoral Position – Digestive Health (University of California, Irvine) Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.
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