Metabolic Programming of Mesenchymal Stromal Cells by Oxygen Tension Directs Chondrogenic Cell Fate Researchers prove that metabolic programming of mesenchymal stromal cells (MSCs) by oxygen tension directs chondrogenesis into either permanent or transient hyaline cartilage. Human MSCs chondrogenically differentiated in vitro under hypoxia produced more hyaline cartilage, which expressed typical articular cartilage biomarkers, including established inhibitors of hypertrophic differentiation. [Proc Natl Acad Sci USA] Abstract Scaffold Mean Pore Size Influences Mesenchymal Stem Cell Chondrogenic Differentiation and Matrix Deposition Scientists investigated whether scaffold mean pore sizes affects mesenchymal stem cell initial attachment, morphology, chondrogenic gene expression and cartilage-like matrix deposition. [Tissue Eng Part A] Abstract A Novel Method for Differentiation of Human Mesenchymal Stem Cells into Smooth Muscle-Like Cells on Clinically Deliverable TIPS Microspheres Scientists report the use of highly porous microcarriers produced using thermally induced phase separation (TIPS) to expand and differentiate adipose-derived mesenchymal stem cells into smooth muscle-like cells in a format that requires minimal manipulation before clinical delivery. [Tissue Eng Part C Methods] Abstract Gene Expression Profile Analysis of Human Mesenchymal Stem Cells from Herniated and Degenerated Intervertebral Discs Reveals Different Expression of Osteopontin Researchers present a characterization of mesenchymal stem cells (MSCs) isolated from clinically-categorized herniated (H-IVD) and degenerated (D-IVD) disc samples. H-IVD-MSCs and D-IVD-MSCs showed multipotent mesenchymal differentiation ability, expressing positivity for adipogenic, osteogenic, and chondrogenic markers with an immunophenotypical profile representative of MSCs. [Stem Cells Dev] Abstract Novel Hedgehog Agonists Promote Osteoblast Differentiation in Mesenchymal Stem Cells The authors demonstrate that hedgehog-Ag 1.3 and 1.7 strongly activate the expression of endogenous Gli1 and promote osteoblast differentiation in the mesenchymal stem cell line C3H10T1/2. [J Cell Physiol] Abstract Decreased SIRT3 in Aged Human Mesenchymal Stromal/Stem Cells Increases Cellular Susceptibility to Oxidative Stress Researchers show that severe oxidative stress reduces sirtuin3 (SIRT3) levels in young human mesenchymal stromal/stem cells (hMSCs). Overexpression of SIRT3 improved hMSCs resistance to the detrimental effects of oxidative stress. [J Cell Mol Med] Full Article Effects of Negative Pressure Wound Therapy on Mesenchymal Stem Cells Proliferation and Osteogenic Differentiation in a Fibrin Matrix Researchers investigated the effects of negative pressure wound therapy on rat periosteum-derived mesenchymal stem cells proliferation and osteoblastic differentiation in a 3D fibrin matrix. [PLoS One] Full Article Mangiferin Reduces the Inhibition of Chondrogenic Differentiation by IL-1β in Mesenchymal Stem Cells from Subchondral Bone and Targets Multiple Aspects of the Smad and SOX9 Pathways Scientists determined the effect of mangiferin on chondrogenic differentiation in IL-1β-stimulated mesenchymal stem cells from subchondral bone and to explore the mechanisms underlying these effects. [Int J Mol Sci] Abstract | Download Full Article The Influence of IL-10 and TNFα on Chondrogenesis of Human Mesenchymal Stromal Cells in Three-Dimensional Cultures Scientists determined the effectiveness of two three-dimensional (3D) culture systems for chondrogenic mesenchymal stromal cell (MSC) differentiation in comparison to primary chondrocytes and to assess the effect of Interleukin (IL)-10 and Tumor Necrosis Factor (TNF)α on chondrogenesis by MSCs in 3D high-density culture. [Int J Mol Sci] Abstract | Download Full Article Human Bone Marrow Stromal Cells Lose Immunosuppressive and Anti-Inflammatory Properties upon Oncogenic Transformation Using sequentially mutated human bone marrow stromal cells (hBMSCs) and spontaneously transformed hBMSCs, scientists report that, upon oncogenic transformation, hBMSCs lose immunosuppressive and anti-inflammatory properties in vitro and in vivo. [Stem Cell Rep] Full Article |