Muscle Cell News 4.11 April 1, 2019 | |
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TOP STORYResearchers used genetic mouse models to delete vascular endothelial growth factor (VEGF) receptors, adeno-associated viral vectors to transduce the myocardium and pharmacological inhibitors to block VEGF and ErbB signaling in vivo. Cell culture experiments were employed for mechanistical studies and qPCR, microarrays, ELISA and immunohistochemistry to analyze the cardiac phenotypes. [Circulation] Abstract | |
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PUBLICATIONS(Ranked by impact factor of the journal)CARDIAC MUSCLE CELLSA Microscopically Motivated Model for the Remodeling of Cardiomyocytes Scientists present a thermodynamically based model that captures the remodeling effects in cardiac muscle cells. This work begins with the formulation of the kinematics of a cardiomyocyte resulting from a prescribed macroscopic deformation and the reorganization of the internal structure. [Biomech Model Mechanobiol] Abstract The authors expressed adiponectin (ADN) in cultured primary adult cardiomyocytes and nonmuscle (COS) cells. After 48 hours of ADN expression by adenovirus treatment, roughly half of synthesized ADN was secreted from cardiomyocytes, and half was still in-transit within inner membrane compartments, similar to COS cells. [Mol Cell Biochem] Abstract SKELETAL MUSCLE CELLSHigh-Dimensional Single-Cell Cartography Reveals Novel Skeletal Muscle-Resident Cell Populations Using a combined approach of single-cell RNA sequencing and mass cytometry, investigators precisely mapped 10 different mononuclear cell types in adult mouse muscle. They also characterized gene signatures and determined key discriminating markers of each cell type. [Mol Cell] Abstract | Graphical Abstract Scientists recruited 12 young and 12 elderly women, who performed a single bout of heavy resistance exercise with the knee extensor muscles of one leg. Five days later, muscle biopsies were collected from both legs and myogenic cells and non-myogenic cells were isolated for in vitro experiments with mixed or separated cells, and analyzed by immunostaining and RT-PCR. [Am J Physiol Cell Physiol] Abstract H3K27me3 Depletion during Differentiation Promotes Myogenic Transcription in Porcine Satellite Cells Porcine satellite cells were isolated and in vitro cultured from newborn piglets. RNA sequencing and chromatin immunoprecipitation sequencing experiments were performed using proliferating cells and terminal myotubes in order to interrogate the transcriptomic profiles, as well as the distribution of histone markers—H3K4me3, H3K27me3, and H3K27ac—and RNA polymerase II. [Genes (Basel)] Full Article Oxidative Hotspots on Actin Promote Skeletal Muscle Weakness in Rheumatoid Arthritis Scientists showed that oxidative post-translational modifications of the contractile machinery targeted to actin result in impaired actin polymerization and reduced force production. Using mass spectrometry, they identified the actin residues targeted by oxidative 3-nitrotyrosine or malondialdehyde adduct modifications in weakened skeletal muscle from mice with arthritis and patients afflicted by rheumatoid arthritis. [JCI Insight] Abstract | Full Article SMOOTH MUSCLE CELLSAmlodipine Induces Vasodilation via Akt2/Sp1‐Activated miR‐21 in Smooth Muscle Cells Real‐time PCR and luciferase assays showed that amlodipine induced miR‐21 overexpression in vascular smooth muscle cells. [Br J Pharmacol] Abstract Scientists demonstrated that miR-132 expression was upregulated in monocrotaline-induced pulmonary arterial hypertension rats and platelet-derived growth factor-induced pulmonary arterial smooth muscle cells. [Mol Med Rep] Abstract Subscribe to one of our other 19 science newsletters such as Extracellular Matrix News & ESC & iPSC News. | |
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REVIEWSSignaling Pathways Involved in Vascular Smooth Muscle Cell Calcification during Hyperphosphatemia The authors address critical intracellular pathways controlling osteo-/chondrogenic transdifferentiation of vascular smooth muscle cells and, thus, vascular calcification during hyperphosphatemia. Elucidating these pathways holds a significant promise to open novel therapeutic opportunities counteracting the progression of vascular calcification in chronic kidney disease. [Cell Mol Life Sci] Full Article Visit our reviews page to see a complete list of reviews in the muscle cell research field. | |
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INDUSTRY NEWSSanthera Pharmaceuticals announced its intention to file an application for Conditional Marketing Authorization for Puldysa® for the treatment of respiratory dysfunction in Duchenne muscular dystrophy with the European Medicines Agency. [Santhera Pharmaceuticals] Press Release New Max Planck-Bristol Centre for Minimal Biology Launched Building stripped-down versions of life using protocells, genome delivery systems and synthetic cytoskeletons comprise some of the groundbreaking research due to take place at a new centre launched at the University of Bristol. [University of Bristol] Press Release | |
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POLICY NEWSWhat Thailand’s Long-Awaited Election Could Mean for Science Thailand is bracing for the results of its first election since a military junta took charge in a 2014 coup. Scientists, like many other people in Thailand, want a more democratic country. But whether a pro-democracy party or a military-backed one ends up in charge, science is likely to prosper as a result of ongoing reforms put in place by the junta that are likely to continue under either government. [Nature News] Editorial National Academy of Sciences Will Vote on Ejecting Sexual Harassers The US National Academy of Sciences in Washington, DC, will ask its members this month to change the organization’s bylaws to allow proven sexual harassers and those guilty of other misconduct to be ejected from their ranks. That’s a first for the prestigious organization that advises the US government on scientific issues: Its members, who are voted in by other members, have always been elected for life. [ScienceInsider] Editorial
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EVENTSNEW Cell Symposia: Engineering Organoids and Organs Visit our events page to see a complete list of events in the community.
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JOB OPPORTUNITIESNEW International Postdoctoral Fellowships – Metabolism (University of Copenhagen) Postdoctoral Scholar – Cardiovascular Genomics (Stanford Medicine) Early Stage Researchers – Various (Institutions across Europe in the TRIM-NET – ITN Network) Postdoctoral Research Associate – Cardiovascular Research (Old Dominion University) Postdoctoral Position – Mechanotransduction in Heart Development and Regeneration (Brown University) Assistant Professor – Sarcoma or Skeletal Related Malignancies (University of California, Davis) Postdoctoral Position – Muscle Stem Cell Biology (University of Sherbrooke) Assistant Specialist – Cardiovascular Research (University of California, Davis) Postdoctoral Research Position – Cardiovascular Medicine (University of California, Davis) Assistant/Associate Adjunct Professor – Cardiovascular Medicine (University of California, Davis) Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.
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