Hematopoiesis News Volume 11.07 | Feb 25 2020

TOP STORY

Alterations in Chromatin at Antigen Receptor Loci Define Lineage Progression during B Lymphopoiesis

Using the micrococcal nuclease-based chromatin accessibility assay with low-cell count input, researchers profiled both small-scale and large-scale changes in chromatin accessibility and nucleosome occupancy in primary cells during lymphoid development, tracking the changes as different antigen receptor loci became primed for recombination. [Proc Natl Acad Sci USA] Full Article

(Ranked by impact factor of the journal)

G-CSF Directly Acts on Mouse Lymphoid-Biased but not Myeloid-Biased Hematopoietic Stem Cells

Scientists performed serum-free single-cell culture and competitive repopulation with cultured cells. Single highly purified hematopoietic stem cells and hematopoietic progenitor cells were cultured with stem cell factor (SCF), SCF + granulocyte colony-stimulating factor (G-CSF), SCF + granulocyte/macrophage-CSF, or SCF + thrombopoietin for seven days. [Haematologica] Abstract

The Nuclear Gene rpl18 Regulates Erythroid Maturation via JAK2-STAT3 Signaling in Zebrafish Model of Diamond-Blackfan Anemia

To genetically model the ribosomal defect identified in Diamond-Blackfan anemia (DBA), researchers generated a rpl18 mutant line in zebrafish, using CRISPR/Cas9 system. Molecular characterization of this mutant line demonstrated that Rpl18 deficiency mirrored the erythroid defects of DBA, namely a lack of mature red blood cells. [Cell Death Dis] Full Article

Transforming Growth Factor β1 Accelerates and Enhances In Vitro Red Blood Cell Formation from Hematopoietic Stem Cells by Stimulating Mitophagy

Investigators examined whether the use of low concentration of transforming growth factor β1 would be beneficial for increasing red blood cell production by stimulating hematopoietic stem cell growth and also supporting erythroid differentiation. [Stem Cell Res Ther] Full Article

Cxxc5 Expression Alters Cell Cycle and Myeloid Differentiation of Mouse Hematopoietic Stem and Progenitor Cells

The role of Cxxc5 in myelopoiesis was studied using overexpression and short hairpin RNA‐mediated knockdown in mouse early stem and progenitor cells defined as Lineage⁻ Sca‐1⁺c‐Kit⁺ cells. [J Leukoc Biol] Abstract

Zika Virus Infection Studies with CD34⁺ Hematopoietic and Megakaryocyte‐Erythroid Progenitors, Red Blood Cells and Platelets

Zika virus RNA replication, protein translation and infectious particle formation in hematopoietic cell lines as well as primary CD34⁺ HSPCs and ex vivo differentiated erythroid and megakaryocytic cells was monitored using qRT‐PCR, FACS, immunofluorescence analysis and infectivity assays. [Transfusion] Full Article

Inhibition of JAK2 Suppresses Myelopoiesis and Atherosclerosis in Apoe^−/− Mice

TG101348 selectively reversed neutrophilia, monocytosis, hematopoietic stem and progenitor cell (HSPC), and granulocyte-macrophage progenitor expansion in Apoe^−/− mice with decreased cellular phosphorylated STAT5 and ERK1/2 and reduced cell cycling and BrdU incorporation in HSPCs, indicating inhibition of JAK/STAT signaling and cell proliferation. [Cardiovasc Drugs Ther] Full Article

Deletion of a Conserved Gata2 Enhancer Impairs Hemogenic Endothelium Programming and Adult Zebrafish Hematopoiesis

The authors deleted a conserved enhancer driving pan-endothelial expression of the zebrafish gata2a and showed that Gata2a was required for hemogenic endothelium programming by regulating expression of runx1 and of the second Gata2 orthologue, gata2b. [Commun Biol] Full Article

CLINICAL RESEARCH

The Role of Donor Lymphocyte Infusion (DLI) in Post Hematopoietic Cell Transplant (HCT) Relapse for Chronic Myeloid Leukemia (CML) in the Tyrosine Kinase Inhibitor (TKI) Era

This study was performed through the Center for International Blood and Marrow Transplant Research (CIBMTR) database. Investigators retrospectively reviewed all patients reported to the CIBMTR registry from 2002-2014 who underwent HCT for CML and were alive 30 days post relapse. [Biol Blood Marrow Transplant] Abstract

Subscribe to one of our other 19 science newsletters such as Cord Blood News & Cell Therapy News.

Hematopoietic Stem Cell Transplantation for Blood Cancers in the Era of Precision Medicine and Immunotherapy

Investigators describe the current role of autologous and allogeneic hematopoietic stem cell transplantation (HCT) in the treatment of patients with acute leukemias, aggressive B‐cell lymphomas, and multiple myeloma and discuss how novel targeted therapies and immunotherapies have changed the potential need, timing, and goal of HCT in patients with these diseases. [Cancer] Abstract

Altering Chromatin Methylation Patterns and the Transcriptional Network Involved in Regulation of Hematopoietic Stem Cell Fate

The authors summarize how dysregulation in the expression of genes related to self‐renewal, proliferation, and differentiation of hematopoietic stem cells caused by changes in epigenetic modifications and transcriptional networks lead to clonal expansion and leukemic transformation. [J Cell Physiol] Abstract

Visit our reviews page to see a complete list of reviews in the hematopoiesis research field.

Magenta Therapeutics Completes Dosing in Phase I MGTA-145 Trial, Demonstrating Rapid, Single-Day First Line Stem Cell Mobilization and Collection

Magenta Therapeutics announced the completion of dosing in its Phase I trial of stem cell mobilization therapy clinical candidate, MGTA-145. [Magenta Therapeutics] Press Release

FORMA Therapeutics Announces FT-4202 Granted Fast Track Designation and Rare Pediatric Disease Designation for Treatment of Sickle Cell Disease

FORMA Therapeutics, Inc. announced the FDA has granted Fast Track designation and Rare Pediatric Disease designation for its lead clinical asset, FT-4202, in development as a disease-modifying treatment for sickle cell disease. [FORMA Therapeutics, Inc.] Press Release

US Food and Drug Administration Accepts Karyopharm’s Supplemental New Drug Application for XPOVIO^® (Selinexor) as a Treatment for Patients with Relapsed or Refractory Diffuse Large B-Cell Lymphoma

Karyopharm Therapeutics, Inc. announced that the FDA has accepted for filing its supplemental New Drug Application seeking accelerated approval for oral XPOVIO^® tablets, the company’s first-in-class, Selective Inhibitor of Nuclear Export compound, for the treatment of adult patients with relapsed or refractory diffuse large B-cell lymphoma, not otherwise specified, who have received at least two prior therapies. [Karyopharm Therapeutics, Inc.] Press Release

White House Formally Invites Public Comment on Open-Access Policies

The White House issued a notice seeking comment on its effort to enhance public access to federally funded research. It’s an old idea creating new controversy. White House Office of Science and Technology Policy Director Kelvin Droegemeier is pushing back against publishers that in December said the administration was quietly pursuing an executive order to require immediate free distribution of taxpayer funded research. [ScienceInsider] Editorial

Has the UK Science Minister Been Demoted?

A government reshuffle in the United Kingdom has born another new science minister – a position that has seen a revolving door of appointees, firings and resignations in recent years. The latest appointment also comes with an apparent political demotion for the role, which expert observers say is in conflict with a government that appears to have put research and innovation at the heart of its agenda. [Nature News] Editorial

NEW British Society for Haematology 60^th Annual Scientific Meeting
April 27-29, 2020
Birmingham, United Kingdom

Visit our events page to see a complete list of events in the community.

NEW Research Associate – Stem Cell and Gene Therapy Program (Fred Hutchinson Cancer Research Center)

Postdoctoral Position – RNA Modifications, Hematopoiesis and Cancer (Lund University)

Assistant/Associate Professor – Hematopoietic Cell Transplant Program (Fred Hutchinson Cancer Research Center)

Postdoctoral Fellow – Plasma Cell Development and Functional Heterogeneity (Western Michigan University)

Postdoctoral Fellow Positions – Hematopoietic Stem Cell Biology (Nationwide Children’s Hospital)

Postdoctoral Researcher – Bioinformatics and Leukemia Stem Cells (The Centre for Genomic Regulation)

Postdoctoral Fellow – Hereditary and Childhood Tumors (The University of British Columbia)

Postdoctoral Fellowship – Personalized Cancer Treatments (MGH Cancer Center)

Assistant/Associate Professor – Hematology & Oncology (UC Davis School of Medicine)

Associate/Professor – Hematology & Oncology (UC Davis School of Medicine)

Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.

Have we missed an important article or publication in Hematopoiesis News? Click here to submit!

Comments or suggestions? Submit your feedback here.