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Pulmonary Cell News| Mesenchymal Cell News 11.16 April 30, 2019 | |
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TOP STORYIdentification of a Mesenchymal Progenitor Cell Hierarchy in Adipose Tissue Researchers used single-cell RNA sequencing to identify distinct types of progenitor cells in murine and human adipose tissues and to predict lineage relationships in an unbiased manner. Functional assessments of these cell types in vitro and in vivo defined a mesenchymal cell hierarchy involved in adipocyte formation. [Science] Abstract | |
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PUBLICATIONS(Ranked by impact factor of the journal)IN VITROA single-step method for extracellular matrix (ECM) functionalization on poly(lactic-co-glycolic acid) microspheres using a novel compound, dopamine-conjugated poly(ethylene-alt-maleic acid), as a stabilizer during the preparation of microspheres was reported. The dopamine molecules on the surface of microspheres provided active sites for the conjugation of ECM in an aqueous solution. [Small] Abstract Ankylosing spondylitis (AS)/β-TCP scaffolds were used as a three-dimensional biomimetic culture system to mimic the bone microenvironment in vivo to determine the abnormal osteogenic differentiation of AS-MSCs. Scientists demonstrated that when cultured in HA/β-TCP scaffolds, AS-MSCs had a stronger osteogenic differentiation capacity than that of MSCs from healthy donors in vitro and in vivo. [Cell Death Dis] Full Article Researchers showed that culture-adapted hepatic stellate cells (HeSCs) expressed substantially higher levels of hepatocyte growth factor, matrix metalloproteinase-1 and chemokine ligand 2 than bone marrow MSCs (BM-MSCs). Both human BM-MSCs and HeSCs inhibited T cell proliferation by a shared indoleamine, 2,3 dioxygenase-dependent mechanism. [Stem Cells] Abstract Investigators compared various preconditioning regimens for both the quantitative and qualitative production of mesenchymal stem cell-derived extracellular vesicles, and their therapeutic efficacy for proangiogenic activity in vitro and cutaneous wound healing in vivo. [J Clin Med] Full Article The authors described a method by which mammalian cells were added to silk solution before assembly, and thereby got uniformly integrated between the formed microfibers. In the resulting 3D scaffold, the cells were highly proliferative and spread out more efficiently than when encapsulated in a hydrogel. Elongated cells containing filamentous actin and defined focal adhesion points confirmed proper cell attachment to the FN-silk. [Sci Rep] Full Article Nestin+ cells were isolated using flow cytometry by gating for CD45−Ter119−CD31− cells from the compact bone of Nestin-GFP transgenic mice, expressing GFP driven by the Nestin promoter. Colony-forming and proliferative curve assays were conducted to determine the proliferative capacity of these cells, while qRT-PCR was used to analyze the mRNA levels of relative chemokines and growth factors. [Stem Cell Res Ther] Abstract IN VIVO4D Anisotropic Skeletal Muscle Tissue Constructs Fabricated by Staircase Effect Strategy The authors translated a staircase defect into an effective bioengineering strategy by integrating fused deposition modeling with surface coating technique to investigate the effect of topographical cues on regulating behaviors of human mesenchymal stem cells toward skeletal muscle tissues. This integrated approach served to fabricate shape-specific, multiple dimensional, anisotropic scaffolds using different biomaterials. [Biofabrication] Abstract Chitosan based hydrogels were developed to host mesenchymal stem cells, since their paracrine action can therapeutically enhance the spinal cord regeneration, limiting the formation of a glial scar and reducing cell death at the injured site. An injectable and highly permeable chitosan-based hydrogel was fabricated having a rapid gelation upon temperature increase from 0 to 37°C. [Sci Rep] Full Article The authors injected rat MSC intravitreally in adult Lister Hooded rats of either sex after optic nerve crush. Retinal ganglion cell survival, axonal regeneration, and reconnection were analyzed 60 and 240-days after crush by immunohistochemistry for Tuj1, anterograde labeling with cholera-toxin B and by immunohistochemistry for nerve growth factor-induced gene A in the superior colliculus after a cycle of light deprivation-stimulation. [Stem Cell Res Ther] Full Article Scientists assess the safety and feasibility of intra‐arterial delivery of autologous MSCs in stromal vascular fraction to the kidney in cats with chronic kidney disease. [J Vet Intern Med] Abstract | Full Article Subscribe to one of our other 19 science newsletters such as ESC & iPSC News & Cell Therapy News. | |
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REVIEWSMimicking the Articular Joint with In Vitro Models The authors discuss the current in vitro models of the joint and the progress achieved in the development of novel and potentially more predictive models, and highlight the application of new technologies to accurately emulate the articular joint. [Trends Biotechnol] Abstract Mesenchymal stem cell (MSC)-based therapy has yielded promising results in repairing hypoxia-reoxygenation – or ischemia-reperfusion injury in various tissues. However, MSC transplantation in vivo must overcome the barriers including the low survival rate of transplanted stem cells, limited targeting capacity, and low grafting potency; therefore, much effort is needed to increase the survival and activity of MSCs in vivo. [Stem Cell Res Ther] Full Article Visit our reviews page to see a complete list of reviews in the mesenchymal cell research field. | |
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SCIENCE NEWSExosomes Derived from Human Mesenchymal Stem Cells Ameliorate Autistic-Like Behaviors Stem Cell Medicine Ltd. presented results of a study of its proprietary mesenchymal stem cell-derived exosomes for the treatment of autistic disorders. A poster presentation titled, “Exosomes Derived from Human Mesenchymal Stem Cells Ameliorates Autistic-like Behaviors in BTBR and Shank3 Mice Models of Autism,” took place. [Press release from Stem Cell Medicine Ltd. discussing research presented at the ISEV2019 Annual Meeting, Kyoto] Press Release BioRestorative Therapies, Inc. announced that Francisco Silva, Vice President of Research and Development will provide an update on BioRestorative’s ThermoStem® program targeted for treating metabolic disorders. [Press release from BioRestorative Therapies, Inc. discussing research to be presented at the 2019 American Society of Gene and Cell Therapy (ASGCT), Washington, DC] Press Release | |
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INDUSTRY NEWSUC Receives Fourth CRISPR Patent; Three More on the Way The US Patent Office issued a fourth patent for the revolutionary CRISPR-Cas9 gene-editing technology to the University of California, expanding the university’s patent portfolio to cover a broad variety of uses in all types of cells as well as cell-free environments. [UC Regents] Press Release MGH Receives $200 Million to Advance Research into Complex Diseases Massachusetts General Hospital (MGH) announced it has received the largest gift in the hospital’s history – $200 million – to endow the Ragon Institute of MGH, MIT and Harvard. This landmark contribution from philanthropists and longtime hospital supporters Phillip T. and Susan M. Ragon comes as the institute marks the 10th anniversary of its founding, made possible by an initial $100 million commitment from the Ragons in 2009. [Massachusetts General Hospital] Press Release | |
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POLICY NEWSEuropean Universities Fail to Report Results for 83% of Trials The vast majority of universities are failing to comply with EU rules on the reporting of clinical trial results, according to a registry analysis. EU rules require sponsors to register clinical trials and post results within 12 months of completing a study. Those rules came into force in 2014, but there is evidence many European universities are yet to adapt to the requirements. [Questex LLC.] Editorial Venezuela Is Unraveling—So Is Its Science Empty labs, nonexistent funding, deteriorating equipment and an unsympathetic government: Many Venezuelan scientists say the country’s worsening political turmoil has made research virtually impossible. In the capital, Caracas, they struggle to find food and maintain personal security—their experiments a distant afterthought. Abroad, self-exiled biochemists and physicists clean houses, wash tables and give private language lessons to make ends meet. [Scientific American] Editorial US Universities Reassess Collaborations with Foreign Scientists in Wake of NIH Letters Kuspa’s attempt to stay ahead of NIH came to naught. A few months into the audit, Baylor College of Medicine received letters from the US National Institutes of Health (NIH) asking about four scientists it believed had violated the agency’s rule requiring them to disclose all foreign ties relating to their research. [ScienceInsider] Editorial Fearing No-Deal Brexit, European Funder Orders UK Researchers to Transfer Grants The prospect of Brexit, the United Kingdom’s departure from the European Union, has loomed long and large over researchers, but the effects on funding, so far, have been speculative. Now, a European funding agency has made a pre-emptive strike in advance of Brexit, changing a policy that directly impacts grants in the United Kingdom. [ScienceInsider] Editorial
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EVENTSNEW The Business of Regenerative Medicine Visit our events page to see a complete list of events in the community.
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JOB OPPORTUNITIESNEW Research Assistant – Sarcoma and Melanoma Cells (MD Anderson Cancer Center) Postdoctoral Fellow – Sarcoma Image Processing (Institute of Cancer Research) Lecturer – Stem Cell Biology & Regenerative Medicine (University of Manchester) Postdoctoral Fellow – Sarcoma Genomics Analysis (The Francis Crick Institute) Postdoctoral – Stem Cell & Biochemical Pharmacology (Genetech) Senior Research Fellow – Stem Cell & Cancer Research (Gillies McIndoe Research Institute) Postdoctoral Fellowship – Molecular Oncology (Max Delbrück Center for Molecular Medicine) Junior Professorship – Translational Pediatric Sarcoma Research (German Research Center) Cell Biologist – MSCs & Liver Disease (Hepatx Corporation) Assistant Professor – Sarcoma or Skeletal Related Malignancies (University of California at Davis) Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.
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