Muscle Cell News Volume 5.00 | Jan 6 2020

TOP STORY

Arterial Sca1⁺ Vascular Stem Cells Generate De Novo Smooth Muscle for Artery Repair and Regeneration

The authors performed cell fate mapping and single-cell RNA sequencing to identify Sca1⁺ vascular stem cells (VSCs) in the adventitial layer of artery walls. After severe injury, Sca1⁺ VSCs migrated into the medial layer and generated de novo smooth muscle cells, which subsequently expanded more efficiently compared with pre-existing smooth muscle. [Cell Stem Cell] Abstract | Graphical Abstract

(Ranked by impact factor of the journal)

CARDIAC MUSCLE CELLS

Single-Cell Analysis Uncovers that Metabolic Reprogramming by ErbB2 Signaling Is Essential for Cardiomyocyte Proliferation in the Regenerating Heart

Researchers uncovered that proliferating border zone cardiomyocytes had very distinct transcriptomes compared to the nonproliferating remote cardiomyocytes and that they resembled embryonic cardiomyocytes. [eLife] Abstract

Ginsenoside Rb1 Exerts Antiarrhythmic Effects by Inhibiting I_Na and I_CaL in Rabbit Ventricular Myocytes

Scientists showed that Rb1 abolished high calcium-induced delayed afterdepolarizations, depressed the increase in intracellular calcium, relieved calcium overload and protected cardiomyocytes. [Sci Rep] Full Article

SKELETAL MUSCLE CELLS

Rainbow Trout Slow Myoblast Cell Culture as a Model to Study Slow Skeletal Muscle and the Characterization of mir-133 and mir-499 Families as a Case Study

Thanks to the compartmentalization of fish muscle fibers the authors developed a slow myoblast cell culture for rainbow trout. Slow and fast muscle-derived myoblasts had similar morphology, but with differential expression of slow muscle markers such as slow myhc, sox6 and pgc-1α. [J Exp Biol] Abstract

Establishment and Validation of Cell Pools Using Primary Muscle Cells Derived from Satellite Cells of Pig Skeletal Muscle

Investigators established three cell pools consisting of M. rhomboideus or M. longissimus from ten or six piglets, each with one gender and medium birth weight. Real-time impedimetric monitoring was used to evaluate the proliferative growth behavior of myoblasts for the cell pools in comparison to their corresponding unpooled cells over a period of 72 hours, with a measurement being taken every 30 minutes. [In Vitro Cell Dev Biol Anim] Full Article

SMOOTH MUSCLE CELLS

Drug‐Eluting Stent Targeting Sp‐1‐Attenuated Restenosis by Engaging YAP‐Mediated Vascular Smooth Muscle Cell Phenotypic Modulation

In cell culture and in rat carotid arterial injury models, specificity protein 1 (Sp‐1) expression was significantly induced, and correlated with smooth muscle cell proliferative phenotype. [J Am Heart Assoc] Full Article

Melatonin Modulates Airway Smooth Muscle Cell Phenotype by Targeting the STAT3/Akt/GSK-3β Pathway in Experimental Asthma

The authors investigated the benefits of melatonin in asthma, with an emphasis on airway remodeling. The results indicated that melatonin significantly attenuated airway hyperresponsiveness, inflammation and remodeling in a house dust mite model. [Cell Tissue Res] Abstract

PKM2-Dependent Glycolysis Promotes the Proliferation and Migration of Vascular Smooth Muscle Cells during Atherosclerosis

Scientists detected pyruvate kinase isoform M2 (PKM2) expression and pyruvate kinase activity in oxidized low-density lipoprotein (oxLDL)-treated vascular smooth muscle cells (VSMCs) and explored the regulation of PKM2 in oxLDL-treated VSMCs and apoE^−/− mice. [Acta Biochim Biophys Sin] Abstract

Small Extracellular Vesicles from Rat Plasma Promote Migration and Proliferation of Vascular Smooth Muscle Cells

Researchers examined the effects of plasma small extracellular vesicles in Wistar Kyoto rats and spontaneously hypertensive rats on the proliferative and migratory functions of primary rat aortic smooth muscle cells. [J Vet Med Sci] Abstract

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The Effects of Iron Overload on Mitochondrial Function, Mitochondrial Dynamics, and Ferroptosis in Cardiomyocytes

The authors summarize and discuss the effects of iron overload on cardiac mitochondrial function, cardiac mitochondrial dynamics, ferroptosis of cardiomyocytes, and left ventricular function in in vitro and in vivo reports. [Arch Biochem Biophys] Abstract | Graphical Abstract

Visit our reviews page to see a complete list of reviews in the muscle cell research field.

Sarepta Therapeutics Announces Partnership with Roche in Territories Outside the United States for Its Investigational Micro-Dystrophin Gene Therapy for Duchenne Muscular Dystrophy, SRP-9001

Sarepta Therapeutics, Inc. announced that Sarepta and Roche have entered into a licensing agreement providing Roche exclusive commercial rights to SRP-9001, Sarepta’s investigational gene therapy for Duchenne muscular dystrophy, outside the United States. [Sarepta Therapeutics, Inc.] Press Release

Cytokinetics Announces Start of Redwood-HCM, a Phase II Clinical Trial of CK-3773274

Cytokinetics, Incorporated announced that REDWOOD-HCM (Randomized Evaluation of Dosing With CK-274 in Obstructive Outflow Disease in HCM) has opened to enrollment. REDWOOD-HCM is a Phase II clinical trial of CK-3773274, a next-generation cardiac myosin inhibitor discovered by company scientists, which Cytokinetics is developing for the potential treatment of hypertrophic cardiomyopathy. [Cytokinetics, Incorporated] Press Release

A Year After the Midterm Elections, Where Are They Now?

November 2018 saw a wave of candidates from all walks of science-engineering, physics, medicine, and other life sciences-win first-time positions in the House, Senate, and state legislatures across the country. Of 20 STEM candidates running for Congressional seats that The Scientist counted last year, 10 won their races, the vast majority of them Democrats as well as political newcomers. [The Scientist] Editorial

NEW FEBS3+ Life Sciences Switzerland
February 13-14, 2020
Zurich, Switzerland

Visit our events page to see a complete list of events in the community.

NEW Doctoral Student – Biomedical Sciences (Linnaeus University)

NEW PhD Student – Aortic Disease (University Hospital of Cologne)

NEW Postdoctoral Research Fellow/Postdoctoral Scientist – Aortic Disease (University Hospital of Cologne)

Postdoctoral Fellow – Computation Systems and Skeletal Muscles (University of Luxembourg)

Postdoctoral Researcher – Muscle Stem Cell Biology (University at Albany)

Postdoctoral Researcher – Skeletal Muscle Aging (St. Jude Children’s Research Hospital)

Senior Scientist – Muscle Cell Biology (Shape Therapeutics)

Asistant Professor – Ion Channels (University of Tennessee)

Assistant Professor – Muscle Biology (Purdue University)

Postdoctoral Researcher – Genome Editing in Cardiac Microtissues (The Jackson Laboratory)

Assistant Specialist – Cardiovascular Medicine (University of California, Davis)

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