Endothelial Cell News Volume 5.16 | May 4 2020

TOP STORY

MicroRNA-21/PDCD4 Proapoptotic Signaling from Circulating CD34⁺ Cells to Vascular Endothelial Cells: A Potential Contributor to Adverse Cardiovascular Outcomes in Patients with Critical Limb Ischemia

In coculture, type 2 diabetes-critical limb ischemia CD34⁺ cells imprinted naïve endothelial cells, increasing apoptosis, reducing network formation, and modulating the TUG1 sponge/miRNA-21/PDCD4 axis. [Diabetes Care] Abstract

(Ranked by impact factor of the journal)

Circular RNA-ZNF532 Regulates Diabetes-Induced Retinal Pericyte Degeneration and Vascular Dysfunction

cZNF532 silencing reduced the viability, proliferation, and differentiation of pericytes and suppressed the recruitment of pericytes toward endothelial cells in vitro. [J Clin Invest] Abstract | Graphical Abstract | Full Article

RhoJ Integrates Attractive and Repulsive Cues in Directional Migration of Endothelial Cells

During angiogenesis, VEGF acts as an attractive cue for endothelial cells (ECs), while Sema3E mediates repulsive cues. Researchers showed that the small GTPase RhoJ integrated these opposing signals in directional EC migration. [EMBO J] Abstract

Id1 and Id3 Maintain Steady-State Hematopoiesis by Promoting Sinusoidal Endothelial Cell Survival and Regeneration

Investigators report that targeted loss of Id genes in adult endothelial cells resulted in dilated, leaky sinusoids and a pro-inflammatory state that increased in severity over time. [Cell Rep] Full Article | Graphical Abstract

Halting the Vicious Cycle within the Multiple Myeloma Ecosystem: Blocking JAM-A on Bone Marrow Endothelial Cells Restores the Angiogenic Homeostasis and Suppresses Tumor Progression

The authors investigated whether bone marrow multiple myeloma endothelial cells (MMECs) control disease progression via the junctional adhesion molecule A (JAM-A). Membrane and cytoplasmic JAM-A levels were upregulated in MMECs in 111 newly diagnosed and 201 relapsed-refractory patients compared to monoclonal gammopathy of undetermined significance and healthy controls. [Haematologica] Abstract

Intravascular Flow Stimulates PKD2 (Polycystin-2) Channels in Endothelial Cells to Reduce Blood Pressure

Researchers generated inducible, endothelial cell (EC)-specific Pkd2 knockout mice to examine vascular functions of PKD2. They showed that a broad range of intravascular flow rates stimulated EC PKD2 channels, producing vasodilation. [eLife] Full Article

Anlotinib Alters Tumor Immune Microenvironment by Downregulating PD-L1 Expression on Vascular Endothelial Cells

Scientists used the specimens of cancer patients for immunohistochemical staining to observe the number of PD-L1⁺ CD34⁺ vascular endothelial cells and infiltrated immune cells inside tumor specimens. [Cell Death Dis] Full Article

Atypical Chemokine Receptor ACKR3/CXCR7 Controls Postnatal Vasculogenesis and Arterial Specification by Mesenchymal Stem Cells via Notch Signaling

CXCR7 gain of function markedly promoted vasculogenesis by mesenchymal stem cells in vitro and in vivo and induced endothelial differentiation along the arterial endothelial cell lineage via upregulation of Notch signaling. [Cell Death Dis] Abstract

Protective Role of Endothelial Calpain Knockout in Lipopolysaccharide-Induced Acute Kidney Injury via Attenuation of the p38-iNOS Pathway and NO/ROS Production

Mouse pulmonary microvascular endothelial cells were used as a model of the microvascular endothelium and were stimulated with lipopolysaccharide. Renal function, renal inducible nitric oxide synthase (iNOS) and endothelial NOS expression, cellular apoptosis, plasma and renal levels of NO and reactive oxygen species (ROS), and phosphorylation of mitogen-activated protein kinase family members were examined. [Exp Mol Med] Full Article

Acute Mechanical Stress in Primary Porcine RPE Cells Induces Angiogenic Factor Expression and In Vitro Angiogenesis

The authors investigated the effect of mechanical stress on the expression of angiogenic factors in porcine retinal pigment epithelial cells and determined the impact of conditioned medium on in vitro angiogenesis. [J Biol Eng] Abstract

Microfluidic Synthesis of Injectable Angiogenic Microgels

Scientists report a fast-gelling and angiogenic collagen scaffold interlocked by ultra-long DNA programmed with vascular endothelial growth factor aptamers. When encapsulated with cells, the angiogenic microgels improved wound healing and liver regeneration, compared with the non-angiogenic or acellular microgels. [Cell Rep Phys Sci] Full Article | Graphical Abstract

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Neuronal and Glial Regulation of CNS Angiogenesis and Barriergenesis

The authors discuss the current understanding of how neuronal and glial development affects central nervous system (CNS) angiogenesis and barriergenesis, and outline future directions to elucidate how neuronal activity might influence these processes. [Development] Abstract

Visit our reviews page to see a complete list of reviews in the endothelial cell research field.

REGENXBIO Announces Additional Positive Long-Term and Interim Phase I/IIa Trial Update for RGX-314 for the Treatment of Wet AMD

REGENXBIO Inc. provided additional long-term data from the ongoing Phase I/IIa trial of RGX-314 for the treatment of wet age-related macular degeneration (wet AMD). [REGENXBIO Inc.] Press Release

BioAge Signs Exclusive License Agreement with Taisho to Develop and Commercialize Taisho’s Phase I HIF-PH Inhibitor to Treat Aging

BioAge Labs, Inc. announced that it has entered into an exclusive worldwide license agreement with Taisho Pharmaceutical Co., Ltd. to develop and commercialize Taisho’s clinical-stage Hypoxia-inducible factor-prolyl hydroxylase (HIF-PH) inhibitor, BGE-117, to ameliorate multiple diseases of aging. [BioAge Labs, Inc. (GlobeNewswire, Inc.)] Press Release

The Pandemic’s Effects on Recruiting International STEM Trainees

Pandemic-related concerns that have arisen for science trainees—shuttered labs, research and education delays, anxiety—are applicable across the board. But international graduate students and postdocs face a suite of unique challenges that raise uncertainty about recruiting and supporting this critical group of researchers in the future. [The Scientist] Editorial

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NEW Postdoctoral Position – Ultrasound-Mediated Phase Transition Contrast Agents for Imaging and Therapy (University of Pittsburgh)

NEW PhD Student Positions – Blood Brain Barrier (Universität Leipzig)

NEW Research Fellow/Scientist – Critical Limb Ischemia (National University of Ireland Galway)

Postdoctoral Positions – Bioinformatics and Vascular Biology (KU Leuven and Sun Yat-Sen University)

Biological Scientist – Lipid Metabolism and Dysregulation (University of Florida)

Research Associate – Cell Culturing (University of California Los Angeles Health)

Postdoctoral Fellow – Precision Oncology (Weill Cornell Medicine)

Postdoctoral Researchers – Cardiovascular Disease and Cancer (Terasaki Institute for Biomedical Innovation)

Postdoctoral Research Fellow – Molecular Oncology (Genentech, Inc.)

Postdoctoral Scientist – Gene Editing in Liver Disease (Beam Therapeutics)

Research Fellow – Cardiovascular Disease (Queen’s University Belfast)

Postdoctoral Researchers – Pancreatic-Endothelial Cell Interactions (German Research Center for Environmental Health)

Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.

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