| Vol. 15.42 – 4 November, 2020 |
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| The authors report the directed differentiation of human iPSCs into airway basal cells, a population resembling the stem cell of the airway epithelium. [Cell Stem Cell] |
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| PUBLICATIONSRanked by the impact factor of the journal |
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| To investigate human neurofibroma pathogenesis, researchers differentiated a series of isogenic patient-specific neurofibromatosis type 1-mutant human iPSCs into Schwannian lineage cells. [Journal of Clinical Investigation] |
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| Scientists profiled microglia differentiated from isogenic, CRISPR-modified TREM2-knockout iPSC lines. [Nature Communications] |
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| The authors applied a tailored experimental design that eliminated confounding effects of differentiation and cell cycle in studying single-cell RNA sequencing, and report thousands of intrinsically covarying gene pairs in mouse ESCs. [Nature Communications] |
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| Endogenous tagging of Nanog in ESCs revealed that ERK inhibition promoted enhanced stabilization of NANOG protein after mitosis. Scientists showed that cell cycle, signaling, and differentiation are coordinated during preimplantation development. [Developmental Cell] |
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| Investigators observed more CD9+C-PEPTIDE+ β cells in the fetal than in the adult cadaveric islets and more Ki67+ proliferating cells among CD9+ fetal β cells. Their experiments showed CD9 as a cell-surface marker for negative enrichment of glucose-responsive β-like cells differentiated from human PSCs. [Stem Cell Reports] |
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| Using a global unbiased proteomics approach, researchers report that embryonic fibroblasts carrying the db/db mutation exhibit metabolic abnormalities, while their reprogrammed iPSCs show altered expression of proteins involved in embryonic development. [Stem Cell Reports] |
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| The authors quantify cell–cell permeability based on dye diffusion using mass transport models. Their results revealed heterogeneous intercellular connectivity and a variety of spatiotemporal characteristics of intercellular Ca2+ waves in human ESC colonies induced by sonoporation of single cells. [Scientific Reports] |
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| Investigators examined the functional in vivo and in vitro effects of frequently detected copy number variations at 20q11.21 during early-stage differentiation of human (h)PSCs. Comprehensive transcriptome profiling of abnormal hPSCs revealed that the differential gene expression patterns had a negative effect on differentiation potential. [Scientific Reports] |
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| Scientists used CRISPR/Cas9 gene editing technology in human (h)PSCs to create a unique suite of four isogenic homozygous variants at amino acid positions 16(G/R) and 27(G/Q), which reside in the N-terminus of the β2AR. [Molecular Therapy-Methods & Clinical Development] |
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| Researchers found the immune regulative protein, gamma-interferon-inducible protein 16 (IFI16) was involved in the regulation of both self-renewal and differentiation gene expression during human ESC trilineage specification through interaction with p53. [NPJ Regenerative Medicine] |
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| Scientists used CAGE-seq to profile all transcription initiation sites in wildtype mouse (m)ESCs and cells lacking PWWP2A/B. Loss of PWWP2A/B enhanced spurious initiation from intragenic sites present in wildtype mESCs, and this effect was associated with increased levels of initiating Pol-II and histone acetylation. [iScience] |
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| The authors present an overview of the current state of pathogenic variant discovery in retinal disease and identify some of the remaining barriers. They also explore the utility of new technologies, specifically patient-derived iPSC-based modeling, in further expanding the catalog of disease-causing variants using transcriptome-focused methods. [Progress in Retinal and Eye Research] |
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| The Stem Cell Network announced $4.3 million in funding to support stem cell and regenerative medicine research projects that will help to deliver improved health for Canadians and will contribute to national economic recovery. Funded projects include stem cell-based research into new therapeutics for type-1 diabetes, cancer, cystic fibrosis, brain and heart conditions. [Stem Cell Network] |
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| April 9 – April 14, 2021 Washington, DC, United States |
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| The Francis Crick Institute – London, England, United Kingdom |
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| Columbia University Medical Center – New York, New York, United States |
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| Ncardia – Leiden, The Netherlands |
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| National University of Ireland Galway – Galway, Ireland |
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| University of California, San Diego- La Jolla, California, United States |
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