Single Cell Transcriptomic Analysis of Human Pluripotent Stem Cell Chondrogenesis

Scientists combined bulk RNA sequencing, single cell RNA sequencing, and bioinformatic analyses, including weighted gene co-expression analysis, to investigate the gene regulatory networks regulating human iPSC differentiation under chondrogenic conditions.
[Nature Communications]
Wu, C.-L., Dicks, A., Steward, N., Tang, R., Katz, D. B., Choi, Y.-R., & Guilak, F. (2021). Single cell transcriptomic analysis of human pluripotent stem cell chondrogenesis. Nature Communications, 12(1), 362. https://doi.org/10.1038/s41467-020-20598-y Cite
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m6A RNA Methylation Regulates the Fate of Endogenous Retroviruses

Using an unbiased genome-scale CRISPR knockout screen in mouse ESCs, investigators identified m6A RNA methylation as a way to restrict endogenous retroviruses.
[Nature]
Chelmicki, T., Roger, E., Teissandier, A., Dura, M., Bonneville, L., Rucli, S., Dossin, F., Fouassier, C., Lameiras, S., & Bourc’his, D. (2021). m 6 A RNA methylation regulates the fate of endogenous retroviruses. Nature, 1–5. https://doi.org/10.1038/s41586-020-03135-1 Cite
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Culture Surface Protein Coatings Affect the Barrier Properties and Calcium Signaling of hESC-RPE

Investigators identified the role of retinal pigment epithelium (RPE) basement membrane proteins type IV collagen, laminin, and nidogen-1 in the maturation and functionality of humn ESC-RPE after cryopreservation.
[Scientific Reports]
Viheriälä, T., Sorvari, J., Ihalainen, T. O., Mörö, A., Grönroos, P., Schlie-Wolter, S., Chichkov, B., Skottman, H., Nymark, S., & Ilmarinen, T. (2021). Culture surface protein coatings affect the barrier properties and calcium signalling of hESC-RPE. Scientific Reports, 11(1), 933. https://doi.org/10.1038/s41598-020-79638-8 Cite
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A Genetic Screen Identifies Etl4-Deficiency Capable of Stabilizing the Haploidy in Embryonic Stem Cells

The authors report a genome-wide loss-of-function screening that identified gene mutations that could significantly reduce the rate of self-diploidization in haploid ESCs.
[Stem Cell Reports]
Zhang, G., Li, X., Sun, Y., Wang, X., Liu, G., & Huang, Y. (2021). A Genetic Screen Identifies Etl4-Deficiency Capable of Stabilizing the Haploidy in Embryonic Stem Cells. Stem Cell Reports, 16(1), 29–38. https://doi.org/10.1016/j.stemcr.2020.11.016 Cite
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Familial Alzheimer’s Disease Mutations in PSEN1 Lead to Premature Human Stem Cell Neurogenesis

Expression data from developmental and adult neurogenesis showed relative enrichment of Notch and γ-secretase expression in stem cells, whereas expression of APP and β-secretase was enriched in neurons.
[Cell Reports]
Arber, C., Lovejoy, C., Harris, L., Willumsen, N., Alatza, A., Casey, J. M., Lines, G., Kerins, C., Mueller, A. K., Zetterberg, H., Hardy, J., Ryan, N. S., Fox, N. C., Lashley, T., & Wray, S. (2021). Familial Alzheimer’s Disease Mutations in PSEN1 Lead to Premature Human Stem Cell Neurogenesis. Cell Reports, 34(2). https://doi.org/10.1016/j.celrep.2020.108615 Cite
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Stem-Cell-Derived Human Microglia Transplanted Into Mouse Brain To Study Human Disease

Scientists detailed an accurate, step-by-step workflow that includes in vitro microglia differentiation from human pluripotent stem cells, transplantation into the mouse brain and quantitative analysis of engraftment.
[Nature Protocols]
Fattorelli, N., Martinez-Muriana, A., Wolfs, L., Geric, I., De Strooper, B., & Mancuso, R. (2021). Stem-cell-derived human microglia transplanted into mouse brain to study human disease. Nature Protocols, 1–22. https://doi.org/10.1038/s41596-020-00447-4 Cite
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Induced Organoids Derived from Patients with Ulcerative Colitis Recapitulate Colitic Reactivity

Researchers took advantage of induced pluripotent stem cells to develop an induced human UC-derived organoid model and compared it with the induced human normal organoid model.
[Nature Communications]
Sarvestani, S. K., Signs, S., Hu, B., Yeu, Y., Feng, H., Ni, Y., Hill, D. R., Fisher, R. C., Ferrandon, S., DeHaan, R. K., Stiene, J., Cruise, M., Hwang, T. H., Shen, X., Spence, J. R., & Huang, E. H. (2021). Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity. Nature Communications, 12(1), 262. https://doi.org/10.1038/s41467-020-20351-5 Cite
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Engineering Human Hepato-Biliary-Pancreatic Organoids from Pluripotent Stem Cells

Investigators describe a protocol for the continuous patterning of hepatic, biliary and pancreatic structures from a 3D culture of human pluripotent stem cells.
[Nature Protocols]
Koike, H., Iwasawa, K., Ouchi, R., Maezawa, M., Kimura, M., Kodaka, A., Nishii, S., Thompson, W. L., & Takebe, T. (2021). Engineering human hepato-biliary-pancreatic organoids from pluripotent stem cells. Nature Protocols, 1–18. https://doi.org/10.1038/s41596-020-00441-w Cite
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BlueRock Therapeutics in Collaboration with Memorial Sloan Kettering Cancer Center Receives IND Clearance for DA01 in Parkinson’s Disease

BlueRock Therapeutics, LLC. announced that the FDA has cleared their Investigational New Drug (IND) application to proceed with a Phase I study in patients with advanced Parkinson’s disease (PD). This is the first trial in the United States to study pluripotent stem cell-derived dopaminergic neurons in patients with PD.
[BlueRock Therapeutics, LLC.]
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Human Erythroid Differentiation Requires VDAC1-Mediated Mitochondrial Clearance

Researchers studied the role of the outer mitochondrial membrane protein VDAC1 in human terminal erythropoiesis. They showed that shRNA-mediated downregulation of VDAC1 accelerated erythroblast maturation.
[Haematologica]
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Human Embryonic Stem Cell-Derived Organoid Retinoblastoma Reveals a Cancerous Origin

The authors describe an innovative retinoblastoma (Rb) organoid model derived from human ESCs with a biallelic mutagenesis of the RB1 gene. The identified tumorigenic growth in the Rb organoids, as well as properties consistent with human primary Rb.
[Proceedings of the National Academy of Sciences of the United States of America]
Liu, H., Zhang, Y., Zhang, Y.-Y., Li, Y.-P., Hua, Z.-Q., Zhang, C.-J., Wu, K.-C., Yu, F., Zhang, Y., Su, J., & Jin, Z.-B. (2020). Human embryonic stem cell-derived organoid retinoblastoma reveals a cancerous origin. Proceedings of the National Academy of Sciences, 117(52), 33628–33638. https://doi.org/10.1073/pnas.2011780117 Cite
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