Category Archives: ESC &amp; iPSC

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A Human iPSC-Derived Inducible Neuronal Model of Niemann-Pick Disease, Type C1

Investigators reported the development of a novel human iPSC-derived, inducible neuronal model of Niemann-Pick disease, type C1, and demonstrated the utility of this new cell line in high-throughput drug/chemical screens to identify potential therapeutic agents.

[BMC Biology]

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Safe and Stable Generation of Induced Pluripotent Stem Cells Using Doggybone DNA Vectors

Researchers reported the first ever application of doggybone DNA (dbDNA™) vectors to generate human iPSCs. They achieved comparable iPSC reprogramming efficiencies using transiently expressing dbDNA™ vectors with the same iPSC reprogramming coding sequences as the state-of-the-art OriP/EBNA1 episomal vectors, in the absence of p53 shRNA repression.

[Molecular Therapy-Methods & Clinical Development]

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Large-Scale Analysis of Imprinting in Naive Human Pluripotent Stem Cells Reveals Recurrent Aberrations and a Potential Link to FGF Signaling

Scientists analyzed loss of imprinting (LOI) in a large cohort of both bulk and single-cell RNA sequencing samples of naive and primed human PSCs (hPSCs). They showed that naive hPSCs exhibited high levels of non-random LOI, with bias toward paternally methylated imprinting control regions.

[Stem Cell Reports]

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Metabolic and Epigenetic Regulation of Endoderm Differentiation

Human PSCs hold great promise for generating endodermal cells and their derivatives for the development of new therapeutics against many life-threatening diseases. Scientists review recent advances in metabolic and epigenetic regulation of endoderm differentiation.

[Trends in Cell Biology]

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Computational Tools for Analyzing Single-Cell Data in Pluripotent Cell Differentiation Studies

The authors focus on computational approaches for the analysis of single-cell omics and imaging data and discuss their use to address many of the major challenges involved in the development, validation, and use of cells obtained from pluripotent cell differentiation.

[Cell Reports Methods]

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Gadd45g Initiates Embryonic Stem Cell Differentiation and Inhibits Breast Cell Carcinogenesis

Researchers showed that the transcripts of Gadd45 family genes, including Gadd45a, Gadd45b, and Gadd45g, were gradually increased upon mouse ESC differentiation. Upregulation of Gadd45 members decreased cell proliferation and induced endodermal and trophectodermal lineages.

[Cell Death Discovery]

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Modeling Congenital Cataract In Vitro Using Patient-Specific Induced Pluripotent Stem Cells

Scientists reported the establishment of a human congenital cataract in vitro model through differentiation of patient-specific iPSCs into regenerated lenses.

[NPJ Regenerative Medicine]

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Enhancer-Associated H3K4 Methylation Safeguards In Vitro Germline Competence

Single-cell RNA sequencing across multiple stages of an in vitro primordial germ cells-like cells (PGCLC) differentiation system showde that PGCLC genes initially expressed in the naïve pluripotent stage became homogeneously dismantled in germline competent epiblast like-cells.

[Nature Communications]

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The ETS Transcription Factor ERF Controls the Exit from the Naïve Pluripotent State in a MAPK-Dependent Manner

The authors examined the molecular mechanisms coordinating the naïve to primed transition by using inducible ESC to genetically eliminate all RAS proteins. They showed that differentiated RASKO ESCs remained trapped in an intermediate state of pluripotency with naïve-associated features.

[Science Advances]

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Mammalian In Vitro Gametogenesis

The past decade has seen considerable advances in in vitro gametogenesis, which aims to recreate germ cell development from PSCs in culture. Mouse PSCs can be induced into functional oocytes and spermatozoa, whereas human PSCs can be induced into early oocytes and prospermatogonia, promoting mechanistic understanding of mammalian germ cell development.

[Science]

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