Investigators showed that the protein kinase D inhibitor CID755673 was able to maintain the undifferentiated state of mouse ESCs in combination with the mitogen-activated protein kinase kinase inhibitor.
Scientists showed that BAP1 was dependent on the ASXL proteins and FOXK1/2 in facilitating gene activation across the genome. Although PR-DUB previously was shown to cooperate with PRC2, they observed minimal overlap and functional interaction between BAP1 and PRC2 in embryonic stem cells.
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Kolovos, P., Nishimura, K., Sankar, A., Sidoli, S., Cloos, P. A., Helin, K., & Christensen, J. (2020). PR-DUB maintains expression of critical genes through FOXK1/2 and ASXL1/2/3-dependent recruitment to chromatin and H2AK119ub1 deubiquitination. Genome Research, gr.261016.120. https://doi.org/10.1101/gr.261016.120 Cite
Calidi Biotherapeutics, Inc., announced that the Investigational New Drug application submitted by its partner, Personalized Stem Cells, Inc. They have received FDA approval for the treatment of COVID-19 and pneumonia patients using stem cell therapy.
[Calidi Biotherapeutics, Inc.]
Researchers showed that AUF1 induced epithelial-to-mesenchymal transition and stemness in breast epithelial cells via stabilization of the SNAIL1 and TWIST1 mRNAs, and their consequent upregulation.
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Cynata Therapeutics Ltd. has announced positive results from the two-year follow-up of patients enrolled in the Phase I clinical trial of CYP-001, Cynata’s lead iPSC-derived Cymerus™ mesenchymal stem cell product.
[Cynata Therapeutics Inc.]
Investigators studied a family affected by dilated cardiomyopathy (DCM) due to a frameshift variant in LMNA. Human induced pluripotent stem cell (iPSC)–derived endothelial cells were generated from patients with LMNA-related DCM and phenotypically characterized.
[Science Translational Medicine]
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Sayed, N., Liu, C., Ameen, M., Himmati, F., Zhang, J. Z., Khanamiri, S., Moonen, J.-R., Wnorowski, A., Cheng, L., Rhee, J.-W., Gaddam, S., Wang, K. C., Sallam, K., Boyd, J. H., Woo, Y. J., Rabinovitch, M., & Wu, J. C. (2020). Clinical trial in a dish using iPSCs shows lovastatin improves endothelial dysfunction and cellular cross-talk in LMNA cardiomyopathy. Science Translational Medicine, 12(554). https://doi.org/10.1126/scitranslmed.aax9276 Cite
iPSC cells are laboratory-produced cells that combine the biological advantages of somatic adult and stem cells for cell-based therapy. The reprogramming of cells, such as fibroblasts, to an ESC-like state is done by the ectopic expression of transcription factors responsible for generating embryonic stem cell properties.
[International Journal of Molecular Sciences]
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Gois Beghini, D., Iwao Horita, S., Cascabulho, C. M., Anastácio Alves, L., & Henriques-Pons, A. (2020). Induced Pluripotent Stem Cells: Hope in the Treatment of Diseases, including Muscular Dystrophies. International Journal of Molecular Sciences, 21(15), 5467. https://doi.org/10.3390/ijms21155467 Cite
The authors developed iPSCs from secondary progressive multiple sclerosis (SPMS)-affected donors and age-matched controls (CT). They confirmed their efficient and similar oligoprogenitor (OPC) differentiation capacity, although they reported SPMS-OPCs were transcriptionally distinguishable from their CT counterparts.
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Researchers measured three pertinent variables in human embryonic stem (hES) cells as a model for the inner cell masses of human blastocysts. They found that RNA encoding the alpha-aminoadipic semialdehyde synthase enzyme, which regulates glutamate production from lysine, was highly expressed in hES cells.
[International Journal of Environmental Research and Public Health]
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Van Winkle, L. J., Galat, V., & Iannaccone, P. M. (2020). Lysine Deprivation during Maternal Consumption of Low-Protein Diets Could Adversely Affect Early Embryo Development and Health in Adulthood. International Journal of Environmental Research and Public Health, 17(15), 5462. https://doi.org/10.3390/ijerph17155462 Cite
To culture PSCs on feeder cell layers, the elimination of feeder cells is required for biological or gene analysis and for cell passage. Therefore, a simple and cost-effective cell sorting technology is required. The authors focused on dielectrophoresis phenomena for cell separation because these do not require any fluorescent or magnetic dyes or antibodies.
CRISPR-Cas9 technology deleted PKD1 in human induced pluripotent stem cell and the cells induced to differentiate along pathways leading to formation of either nephron progenitor or uteric bud organoids.
[Journal of the American Society of Nephrology]
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Kuraoka, S., Tanigawa, S., Taguchi, A., Hotta, A., Nakazato, H., Osafune, K., Kobayashi, A., & Nishinakamura, R. (2020). PKD1-Dependent Renal Cystogenesis in Human Induced Pluripotent Stem Cell-Derived Ureteric Bud/Collecting Duct Organoids. Journal of the American Society of Nephrology. https://doi.org/10.1681/ASN.2020030378 Cite
Research advocates were eager to learn who Health and Human Services Secretary Alex Azar would appoint to the board and to see its ideological makeup. On July 31st they got their first look as it gathered online for a one-time meeting run by the National Institutes of Health.