Minimal Contribution of IP3R2 in Cardiac Differentiation and Derived Ventricular-Like Myocytes from Human Embryonic Stem Cells

Scientists investigated the role of Type 2 inositol 1,4,5-trisphosphate receptor (IP3R2) in the differentiation of human embryonic stem cells (hESCs) to cardiomyocytes and in the hESC-derived cardiomyocytes.
[Acta Pharmacologica Sinica]
Zhang, P., Huang, J., Ou-yang, K., Liang, H., Li, M., Wang, Y., & Yang, H. (2020). Minimal contribution of IP 3 R2 in cardiac differentiation and derived ventricular-like myocytes from human embryonic stem cells. Acta Pharmacologica Sinica, 1–11. https://doi.org/10.1038/s41401-020-00528-w Cite
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Generation of an Immortalized Erythroid Cell Line from Hematopoietic Stem Cells of a Hemoglobin E/β-Thalassemia Patient

Researchers generated an immortalized erythroid cell line from peripheral blood stem cells of a HbE/β-thalassemia patient. Morphological analysis showed the cells are proerythroblasts with some early basophilic erythroblasts, with no change in morphology over time in culture.
[Scientific Reports]
Trakarnsanga, K., Tipgomut, C., Metheetrairut, C., Wattanapanitch, M., Khuhapinant, A., Poldee, S., Kurita, R., Nakamura, Y., Srisawat, C., & Frayne, J. (2020). Generation of an immortalised erythroid cell line from haematopoietic stem cells of a haemoglobin E/β-thalassemia patient. Scientific Reports, 10(1), 16798. https://doi.org/10.1038/s41598-020-73991-4 Cite
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Derivation of Three Human Induced Pluripotent Stem Cell Lines (JUCTCi014-A, JUCTCi015-A, JUCTCi016-a) from Mesenchymal Stem Cells (MSCs) Derived from Bone Marrow, Adipose Tissue and Wharton’s Jelly Samples

Researchers created human induced pluripotent stem cells (hiPSCs) from different MSC sources to evaluate the capacity of MSC-derived iPSCs to differentiate into any cell type of the human body and to serve as an alternative source for iPSC generation.
[Journal of Materials Chemistry B]
Ababneh, N. A., Al-Kurdi, B., Barham, R., Ali, D., Sharar, N., Abuarqoub, D., Jafar, H., Salah, B., & Awidi, A. (2020). Derivation of three human induced pluripotent stem cell lines (JUCTCi014-A, JUCTCi015-A, JUCTCi016-A) from mesenchymal stem cells (MSCs) derived from bone marrow, adipose tissue and Wharton’s jelly samples. Stem Cell Research, 49, 102000. https://doi.org/10.1016/j.scr.2020.102000 Cite
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Parallel Single-Cell RNA-Seq and Genetic Recording Reveals Lineage Decisions in Developing Embryoid Bodies

Investigators provide a high-resolution lineage map for an organoid model of embryogenesis, insights into epigenetic determinants of fate specification, and a strategy for lineage mapping of rapid differentiation processes.
[Cell Reports]
Kim, I. S., Wu, J., Rahme, G. J., Battaglia, S., Dixit, A., Gaskell, E., Chen, H., Pinello, L., & Bernstein, B. E. (2020). Parallel Single-Cell RNA-Seq and Genetic Recording Reveals Lineage Decisions in Developing Embryoid Bodies. Cell Reports, 33(1). https://doi.org/10.1016/j.celrep.2020.108222 Cite
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PICALM Rescues Endocytic Defects Caused by the Alzheimer’s Disease Risk Factor APOE4

In iPSC-derived human astrocytes, increasing expression of PICALM reversed endocytic disruptions. The authors identified a functional interaction between two Alzheimer’s disease genetic risk factors—APOE4 and PICALM—centered on the conserved biological process of endocytosis.
[Cell Reports]
Narayan, P., Sienski, G., Bonner, J. M., Lin, Y.-T., Seo, J., Baru, V., Haque, A., Milo, B., Akay, L. A., Graziosi, A., Freyzon, Y., Landgraf, D., Hesse, W. R., Valastyan, J., Barrasa, M. I., Tsai, L.-H., & Lindquist, S. (2020). PICALM Rescues Endocytic Defects Caused by the Alzheimer’s Disease Risk Factor APOE4. Cell Reports, 33(1). https://doi.org/10.1016/j.celrep.2020.108224 Cite
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Allele Biotech’s cGMP iPSCs Used as Packaging Factories for Dual COVID Vaccine

Allele Biotechnology and Pharmaceuticals, Inc. has been using mRNA as its core technology for creating cGMP-grade iPSC lines for regenerative medicine and oncology for over a decade. The have recently shown that mRNAs encoding the SARS-CoV-2 antigen proteins could be packaged in iPSCs and delivered in extracellular vesicles, potentially together with the viral proteins these mRNAs encode—a dual mRNA and protein vaccine, all naturally packaged in cells.
[Allele Biotechnology and Pharmaceuticals, Inc. (Businesswire, Inc.)]
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Critical Regulation of a NDIME/MEF2C Axis in Embryonic Stem Cell Neural Differentiation and Autism

The authors report that a brain‐enriched long non‐coding RNA, NDIME, is located near the MEF2C locus and is required for normal neural differentiation of mouse ESCs.
[EMBO Reports]
Bai, M., Ye, D., Guo, X., Xi, J., Liu, N., Wu, Y., Jia, W., Wang, G., Chen, W., Li, G., Jiapaer, Z., & Kang, J. (2020). Critical regulation of a NDIME/MEF2C axis in embryonic stem cell neural differentiation and autism. EMBO Reports, n/a(n/a), e50283. https://doi.org/10.15252/embr.202050283 Cite
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Effects of Fibrillin Mutations on the Behavior of Heart Muscle Cells in Marfan Syndrome

Both induced pluripotent stem cells derived from a Marfan syndrome (MFS)-patient and the line with the corrected fibrillin-1 mutation were differentiated to cardiomyocytes. Several functional analyses were performed on this model to study MFS related cardiomyopathy.
[Scientific Reports]
Aalders, J., Léger, L., Van der Meeren, L., Van den Vreken, N., Skirtach, A. G., Sinha, S., De Backer, J., & van Hengel, J. (2020). Effects of fibrillin mutations on the behavior of heart muscle cells in Marfan syndrome. Scientific Reports, 10(1), 16756. https://doi.org/10.1038/s41598-020-73802-w Cite
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ESC-sEVs Rejuvenate Aging Hippocampal NSCs by Transferring SMADs to Regulate the MYT1-Egln3-Sirt1 Axis

Researchers indicated that hippocampal-neural stem cells (NSCs) senescence caused cellular exhaustion, neurogenesis reduction and cognitive impairment during aging, which can be reversed by ESC-small extracellular vesicles (sEVs).
[Molecular Therapy]
Hu, G., Xia, Y., Chen, B., Zhang, J., Gong, L., Chen, Y., Li, Q., Wang, Y., & Deng, Z. (2020). ESC-sEVs rejuvenate aging hippocampal NSCs by transferring SMADs to regulate the MYT1-Egln3-Sirt1 axis. Molecular Therapy, 0(0). https://doi.org/10.1016/j.ymthe.2020.09.037 Cite
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Functional Role of Tet-Mediated RNA Hydroxymethylcytosine in Mouse ESCs and during Differentiation

Investigators proposed that RNA cytosine 5-hydroxymethylation by Tets is a mark of transcriptome flexibility, inextricably linked to the balance between pluripotency and lineage commitment.
[Nature Communications]
Lan, J., Rajan, N., Bizet, M., Penning, A., Singh, N. K., Guallar, D., Calonne, E., Li Greci, A., Bonvin, E., Deplus, R., Hsu, P. J., Nachtergaele, S., Ma, C., Song, R., Fuentes-Iglesias, A., Hassabi, B., Putmans, P., Mies, F., Menschaert, G., … Fuks, F. (2020). Functional role of Tet-mediated RNA hydroxymethylcytosine in mouse ES cells and during differentiation. Nature Communications, 11(1), 4956. https://doi.org/10.1038/s41467-020-18729-6 Cite
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Hyaluronan Regulates Synapse Formation and Function in Developing Neural Networks

Scientists used 3D cortical spheroids derived from human iPSCs to replicate the neurodevelopmental window. Their results demonstrated that hyaluronan preferentially surrounds nascent excitatory synapses.
[Scientific Reports]
Wilson, E., Knudson, W., & Newell-Litwa, K. (2020). Hyaluronan regulates synapse formation and function in developing neural networks. Scientific Reports, 10(1), 16459. https://doi.org/10.1038/s41598-020-73177-y Cite
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