Scientists combined bulk RNA sequencing, single cell RNA sequencing, and bioinformatic analyses, including weighted gene co-expression analysis, to investigate the gene regulatory networks regulating human iPSC differentiation under chondrogenic conditions.
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Using an unbiased genome-scale CRISPR knockout screen in mouse ESCs, investigators identified m6A RNA methylation as a way to restrict endogenous retroviruses.
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Investigators identified the role of retinal pigment epithelium (RPE) basement membrane proteins type IV collagen, laminin, and nidogen-1 in the maturation and functionality of humn ESC-RPE after cryopreservation.
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Viheriälä, T., Sorvari, J., Ihalainen, T. O., Mörö, A., Grönroos, P., Schlie-Wolter, S., Chichkov, B., Skottman, H., Nymark, S., & Ilmarinen, T. (2021). Culture surface protein coatings affect the barrier properties and calcium signalling of hESC-RPE. Scientific Reports, 11(1), 933. https://doi.org/10.1038/s41598-020-79638-8 Cite
The authors report a genome-wide loss-of-function screening that identified gene mutations that could significantly reduce the rate of self-diploidization in haploid ESCs.
[Stem Cell Reports]
Expression data from developmental and adult neurogenesis showed relative enrichment of Notch and γ-secretase expression in stem cells, whereas expression of APP and β-secretase was enriched in neurons.
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Arber, C., Lovejoy, C., Harris, L., Willumsen, N., Alatza, A., Casey, J. M., Lines, G., Kerins, C., Mueller, A. K., Zetterberg, H., Hardy, J., Ryan, N. S., Fox, N. C., Lashley, T., & Wray, S. (2021). Familial Alzheimer’s Disease Mutations in PSEN1 Lead to Premature Human Stem Cell Neurogenesis. Cell Reports, 34(2). https://doi.org/10.1016/j.celrep.2020.108615 Cite
Scientists detailed an accurate, step-by-step workflow that includes in vitro microglia differentiation from human pluripotent stem cells, transplantation into the mouse brain and quantitative analysis of engraftment.
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Researchers took advantage of induced pluripotent stem cells to develop an induced human UC-derived organoid model and compared it with the induced human normal organoid model.
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Sarvestani, S. K., Signs, S., Hu, B., Yeu, Y., Feng, H., Ni, Y., Hill, D. R., Fisher, R. C., Ferrandon, S., DeHaan, R. K., Stiene, J., Cruise, M., Hwang, T. H., Shen, X., Spence, J. R., & Huang, E. H. (2021). Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity. Nature Communications, 12(1), 262. https://doi.org/10.1038/s41467-020-20351-5 Cite
Investigators describe a protocol for the continuous patterning of hepatic, biliary and pancreatic structures from a 3D culture of human pluripotent stem cells.
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BlueRock Therapeutics, LLC. announced that the FDA has cleared their Investigational New Drug (IND) application to proceed with a Phase I study in patients with advanced Parkinson’s disease (PD). This is the first trial in the United States to study pluripotent stem cell-derived dopaminergic neurons in patients with PD.
[BlueRock Therapeutics, LLC.]
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Researchers studied the role of the outer mitochondrial membrane protein VDAC1 in human terminal erythropoiesis. They showed that shRNA-mediated downregulation of VDAC1 accelerated erythroblast maturation.
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The authors describe an innovative retinoblastoma (Rb) organoid model derived from human ESCs with a biallelic mutagenesis of the RB1 gene. The identified tumorigenic growth in the Rb organoids, as well as properties consistent with human primary Rb.
[Proceedings of the National Academy of Sciences of the United States of America]
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Liu, H., Zhang, Y., Zhang, Y.-Y., Li, Y.-P., Hua, Z.-Q., Zhang, C.-J., Wu, K.-C., Yu, F., Zhang, Y., Su, J., & Jin, Z.-B. (2020). Human embryonic stem cell-derived organoid retinoblastoma reveals a cancerous origin. Proceedings of the National Academy of Sciences, 117(52), 33628–33638. https://doi.org/10.1073/pnas.2011780117 Cite