To eliminate glycan holes and mimic the glycosylation of native BG505 Env, researchers replaced all 12 NxS sequons on BG505 SOSIP with NxT.
[Cell Reports]
Derking, R., Allen, J. D., Cottrell, C. A., Sliepen, K., Seabright, G. E., Lee, W.-H., Aldon, Y., Rantalainen, K., Antanasijevic, A., Copps, J., Yasmeen, A., Cupo, A., Portillo, V. M. C., Poniman, M., Bol, N., Woude, P. van der, Taeye, S. W. de, Kerkhof, T. L. G. M. van den, Klasse, P. J., … Sanders, R. W. (2021). Enhancing glycan occupancy of soluble HIV-1 envelope trimers to mimic the native viral spike. Cell Reports, 35(1). https://doi.org/10.1016/j.celrep.2021.108933 Cite
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