Prostate Cancer Originating in Basal Cells Progresses to Adenocarcinoma Propagated by Luminal-Like Cells Researchers present data indicating that advanced prostate adenocarcinoma initiated in basal cells can be maintained by luminal-like tumor-propagating cells. [Proc Natl Acad Sci USA] Abstract | Press Release Hypomethylation Signature of Tumor-Initiating Cells Predicts Poor Prognosis of Ovarian Cancer Patients Investigators analyzed the methylomic profiles of ovarian tumor-initiating cells (CP70sps) and their derived progeny using a human methylation array. qRT-PCR, quantitative methylation-specific PCR, and pyrosequencing were used to verify gene expression and DNA methylation in cancer cell lines. [Hum Mol Genet] Abstract Modeling of Cancer Metastasis and Drug Resistance via Biomimetic Nano-Cilia and Microfluidics Learning from the nature of cilia in lungs – clearing mucus and pathogens from the airway – investigators developed a three-dimensional culture approach via flexible and kinetic copolymer-based chains (nano-cilia) for diminishing cell-to-substrate adhesion. Multicellular spheroids or colonies were tested for 3-7 days in a microenvironment consisting of generated cells with properties of putative cancer stem cells. [Biomaterials] Abstract Hypoxia after Liver Surgery Imposes an Aggressive Cancer Stem Cell Phenotype on Residual Tumor Cells Hypoxia-induced changes in stem cell and differentiation marker expression, clone-forming potential, and metastatic capacity were assessed. The effect of vascular clamping on cancer stem cell characteristics was performed in mice bearing patient-derived liver metastases. [Ann Surg] Abstract Prolactin Induces Notch Signaling in a Jak2-STAT and Jak2-ERK Pathways in Colon Cancer Stem and Progenitor Cells Scientists determined the expression of prolactin and prolactin-receptor (PRLR) in colon cancer tissue and cell lines. Higher levels of PRLR expression were observed in the cancers and cell lines compared to normal colonic epithelial cells. [Carcinogenesis] Abstract Integrated Chromosome 19 Transcriptomic and Proteomic Datasets Derived from Glioma Cancer Stem Cell Lines Researchers performed RNA-Seq, label-free quantitative protein measurements in six glioma-derived cancer stem cell (GSC) lines, and targeted transcriptomic analysis using a chromosome 19-specific microarray in an additional six GSC lines. [J Proteome Res] Abstract Bone Morphogenetic Protein Signaling Induces Astrocytic Differentiation of Clinically-Derived Oligodendroglioma Propagating Cells Scientists successfully isolated and propagated glioma stem-like cells from multiple clinical oligodendroglioma specimens. These oligodendroglioma propagating cells are multipotent and form xenografts with oligodendroglioma features. [Mol Cancer Res] Abstract Cancer Stem-Like Cell Characteristics Induced by EB Virus-Encoded LMP1 Contribute to Radioresistance in Nasopharyngeal Carcinoma by Suppressing the p53-Mediated Apoptosis Pathway Researchers found that latent membrane protein 1 (LMP1) up-regulated the expression of several stemness-related genes, increased the cell number of side population by flow cytometry analysis, enhanced the self-renewal properties of the cells in a spherical culture and enhanced the in vivo tumor initiation ability. [Cancer Lett] Abstract Beta-Catenin/HuR Post-Transcriptional Machinery Governs Cancer Stem Cell Features in Response to Hypoxia Scientists showed that the onset of hypoxia-induced cancer stem cell features requires the beta-catenin-dependent post-transcriptional up-regulation of CA9 and SNAI2 gene expression. [PLoS One] Full Article Downregulation of miR-200a Induces EMT Phenotypes and CSC-Like Signatures through Targeting the β-catenin Pathway in Hepatic Oval Cells By lentivirus-mediated loss-of-function studies, scientists showed that stable knockdown of miR-200a confers a mesenchymal phenotype to WB-F344 cells, including an elongated cell morphology, enhanced cell migration ability and expression of epithelial mesenchymal transition (EMT)-representative markers. Concomitantly, several cancer stem cell (CSC)-like traits appeared in these cells, which exhibit enhanced spheroid-forming capacity, express putative hepatic CSC markers and display superior resistance to chemotherapeutic drugs in vitro. [PLoS One] Full Article |