SCIENCE NEWSAdult Kidney Stem Cells Found in Fish It has long been a given that adult humans — and mammals in general — lack the capacity to grow new nephrons. Researchers have now identified adult kidney stem cells in the zebrafish that can generate new nephrons. These cells can be transplanted from one fish to another fish, whereby they grow into functional nephrons in the transplanted recipient. [Press release from Harvard University discussing online prepublication in Nature]Uncovering the Trail Behind Growing Too Old, Too Soon Scientists have produced the world's first human cell model of progeria. The team used a novel technique of deriving induced pluripotent stem (iPS) cells from cells of human progeria patients. The researchers used their iPS cells to identify two types of cells — mesenchymal stem cells and vascular smooth muscle cells — that were particularly adversely affected by progeria. [Press release from the Agency for Science, Technology and Research (A*STAR) discussing online prepublication in Cell Stem Cell] Red Blood Cell Hormone Modulates the Immune System New research reveals that a hormone best known for stimulating the production of red blood cells can modulate the immune response. The study finds that erythropoietin has contrasting influences on infectious and inflammatory diseases and may be useful in the design of new therapeutic strategies. [Press release from ScienceDaily discussing online prepublication in Immunity] NIH Researchers Extend Use of Gene Therapy to Treat a Soft Tissue Tumor This study is the first to use genetically modified immune cells, in a technique known as adoptive therapy, to cause cancer regression in patients with a solid cancer as opposed to melanoma. This approach represents a method for obtaining immune cells from any cancer patient and converting them into ones that can recognize cancer cells expressing the target antigen, NY-ESO-1, according to researchers. [Press release from the National Institutes of Health discussing online prepublication in the Journal of Clinical Oncology] Study Raises Safety Concerns About Experimental Cancer Approach A study has raised safety concerns about an investigational approach to treating cancer. The strategy takes aim at a key signaling pathway, called Notch, involved in forming new blood vessels that feed tumor growth. When researchers targeted the Notch1 signaling pathway in mice, the animals developed vascular tumors, primarily in the liver, which led to massive hemorrhages that caused their death. [Press release from Washington University School of Medicine in St. Louis discussing online prepublication in the Journal of Clinical Investigation] At Last, a Function at the Junction–Researchers Discover That Stem Cell Marker Regulates Synapse Formation Among stem cell biologists there are few better-known proteins than nestin, whose very presence in an immature cell identifies it as a stem cell. As helpful as this is to researchers, until now no one knew which purpose nestin serves in a cell. Investigators show that nestin has reason for being in a completely different cell type–muscle tissue. There, it regulates formation of the so-called neuromuscular junction, the contact point between muscle cells and “their” motor neurons. [Press release from the Salk Institute of Biological Studies discussing online prepublication in Nature Neuroscience] Roundworm Unlocks Pancreatic Cancer Pathway A team of researchers took a step back to a simpler organism — a common roundworm — and made a discovery about how the Ras oncogene chooses a signaling pathway and how the consequences of that choice play out in cellular development — a key issue in cancer, which is characterized by uncontrolled cell growth. [Press release from University of North Carolina Lineberger Comprehensive Cancer Center discussing online prepublication in Developmental Cell] Growth-Factor-Containing Nanoparticles Accelerate Healing of Chronic Wounds Investigators have developed a novel system for delivery of growth factors to chronic wounds such as pressure sores and diabetic foot ulcers. In their work, the team reports fabricating nanospheres containing keratinocyte growth factor, a protein known to play an important role in wound healing, fused with elastin-like peptides. [Press release from Massachusetts General Hospital discussing online prepublication in the Proceedings of the National Academy of Sciences USA] Research into Synthetic Antibodies Offers Hope for New Diagnostics In a pair of new papers, researchers demonstrated a simple means of improving the binding affinity of synbodies, which are composed of 20 unit chains of amino acids, strung together in random order. They also used random peptide sequences spotted onto glass microarray slides to mine information concerning the active regions or epitopes of naturally occurring antibodies. [Press release from the Biodesign Institute at Arizona State University discussing online prepublication in Molecular and Cellular Proteomics and PLoS One] Novel Effort to Fight Cancer with Cancer Cells Researchers are taking tumor cells from mice, encapsulating them in agarose beads, and implanting them in the abdomen of cancer patients. There, cells in the beads secrete proteins researchers believe could signal a patient's cancer to stop growing, shrink or even die. [Press release from The Rogosin Institute discussing online prepublication in Cancer Research] With Chemical Modification, Stable RNA Nanoparticles Go 3D By replacing a chemical group in RNA, researchers have found a way to bypass RNase and create stable three dimensional configurations of RNA, greatly expanding the possibilities for RNA in nanotechnology. [Press release from the University of Cincinnati discussing online prepublication in ACS Nano] New 3D Nanoscaffold Could Revolutionize Human Tissue Engineering Researchers have created a three-dimensional electrospun scaffold on the nano scale that more effectively and efficiently facilitates cell and tissue growth in the laboratory. [Press release from the University of Alabama at Birmingham's discussing online prepublication in Biomaterials] New Anti-HIV Gene Therapy Makes T-Cells Resistant to HIV Infection An innovative genetic strategy for rendering T-cells resistant to HIV infection without affecting their normal growth and activity is described. A team of researchers from Japan, Korea, and the U.S. developed an anti-HIV gene therapy method in which a bacterial gene called mazF is transferred into CD4+ T-cells. [Press release from ScienceDaily discussing online prepublication in Human Gene Therapy] Caffeine Energizes Cells, Boosting Virus Production for Gene Therapy Applications Give caffeine to cells engineered to produce viruses used for gene therapy and the cells can generate 3- to 8-times more virus, according to a paper. This simple and inexpensive strategy for increasing lentivirus production was developed by researchers from the University of Texas Southwestern Medical Center, Dallas. [Press release from ScienceDaily discussing online prepublication in Human Gene Therapy] Researchers Eliminate Major Roadblock in Regenerative Medicine A team of stem cell biologists and engineers has identified an optimal combination and concentration of small-molecule inhibitors to support the long-term quality and maintenance of human embryonic stem cells in feeder-free and serum-free conditions. [Press release from the University of California, Los Angeles discussing online prepublication in Nature Communications] Chinese Academy of Sciences Initiates “Trailblazing” Stem Cell Research Project The Chinese Academy of Sciences (CAS) announced it has initiated a “strategic, trailblazing” research project on stem cells and regenerative medicine. The project mainly aims to remove the bottlenecks China is confronted with in stem cell research, the CAS said in its 2011 work meeting in Beijing. [Chinese Academy of Sciences Press Release] CURRENT PUBLICATIONS (Ranked by Impact Factor of the Journal)
Identification of Adult Nephron Progenitors Capable of Kidney Regeneration in Zebrafish The data demonstrate that the zebrafish kidney probably contains self-renewing nephron stem/progenitor cells. The identification of these cells paves the way to isolating or engineering the equivalent cells in mammals and developing novel renal regenerative therapies. [Nature] A Human iPSC Model of Hutchinson Gilford Progeria Reveals Vascular Smooth Muscle and Mesenchymal Stem Cell Defects Because mesenchymal stem cells (MSCs) reside in low oxygen niches in vivo, researchers propose that, in Hutchinson-Gilford Progeria syndrome, this causes additional depletion of the MSC pool responsible for replacing differentiated cells lost to progerin toxicity. [Cell Stem Cell] Erythropoietin Contrastingly Affects Bacterial Infection and Experimental Colitis by Inhibiting Nuclear Factor-KappaB-Inducible Immune Pathways Researchers show that erythropoietin (EPO) inhibits the induction of proinflammatory genes including tumor necrosis factor-alpha and inducible nitric oxide synthase in activated macrophages, which is mechanistically attributable to blockage of nuclear factor-B p65 activation by EPO. [Immunity] Conversion of Mouse Fibroblasts into Cardiomyocytes Using a Direct Reprogramming Strategy Researchers show that conventional reprogramming towards pluripotency through overexpression of Oct4, Sox2, Klf4 and c-Myc can be shortcut and directed towards cardiogenesis in a fast and efficient manner. [Nat Cell Biol] Tumor Regression in Patients with Metastatic Synovial Sarcoma and Melanoma Using Engineered Lymphocytes Reactive with NY-ESO-1 Observations indicate that T-cell receptor-based gene therapies directed against NY-ESO-1 represent a new and effective therapeutic approach for patients with melanoma and synovial cell sarcoma. [J Clin Oncol] Notch1 Loss of Heterozygosity Causes Vascular Tumors and Lethal Hemorrhage in Mice Researchers report that the loss of Notch1 caused widespread vascular tumors and organism lethality secondary to massive hemorrhage. These findings reflected a cell-autonomous role for Notch1 in suppressing neoplasia in the vascular system and provide a model by which to explore the mechanism of neoplastic transformation of endothelial cells. [J Clin Invest] Nestin Negatively Regulates Postsynaptic Differentiation of the Neuromuscular Synapse Results suggest that nestin is required for acetylcholine (Ach)-induced, Cdk5-dependent dispersion of ACh receptor clusters during neuromuscular junction development. [Nat Neurosci] Ras Effector Switching Promotes Divergent Cell Fates in C. elegans Vulval Patterning Observations define the utility of Ras effector switching during normal development and may provide a possible mechanistic basis for cell and cancer-type differences in effector dependency and activation. [Dev Cell] Self-Assembling Elastin-Like Peptides Growth Factor Chimeric Nanoparticles for the Treatment of Chronic Wounds Researchers demonstrate the fabrication of a fusion protein comprising of elastin-like peptides and keratinocyte growth factor. [Proc Natl Acad Sci U S A] An Optimized Small Molecule Inhibitor Cocktail Supports Long-Term Maintenance of Human Embryonic Stem Cells Researchers identify, using a feedback system control scheme, a unique combination of three small molecule inhibitors that enables the maintenance of human embryonic stem cells on a fibronectin-coated surface through single cell passaging. [Nat Commun]
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