Interleukin-2 and Regulatory T Cells in Graft-Versus-Host Disease Scientists hypothesized that low-dose interleukin-2 could preferentially enhance regulatory T cells in vivo and suppress clinical manifestations of chronic graft-versus-host disease. [New Engl J Med] Abstract | Press ReleaseScreening Ethnically Diverse Human Embryonic Stem Cells Identifies a Chromosome 20 Minimal Amplicon Conferring Growth Advantage The International Stem Cell Initiative analyzed 125 human embryonic stem cell lines and 11 induced pluripotent stem cell lines, from 38 laboratories worldwide, for genetic changes occurring during culture. [Nat Biotechnol] Abstract | Press Release Adult Cardiac-Resident Mesenchymal Stem Cell-Like Stem Cells with a Proepicardial Origin Here researchers describe a population of adult cardiac-resident colony-forming unit-fibroblasts that occupy a perivascular, adventitial niche and show broad trans-germ layer potency in vitro and in vivo. [Cell Stem Cell] Abstract | Press Release Reprogramming Factor Stoichiometry Influences the Epigenetic State and Biological Properties of Induced Pluripotent Stem Cells Investigators compared two genetically and highly defined transgenic systems to identify parameters affecting reprogramming of somatic cells to a pluripotent state. [Cell Stem Cell] Abstract | Press Release
Human Dental Pulp-Derived Stem Cells Promote Locomotor Recovery After Complete Transection of the Rat Spinal Cord by Multiple Neuro-Regenerative Mechanisms Here scientists report that transplantation of human dental pulp stem cells into the completely transected adult rat spinal cord resulted in marked recovery of hind limb locomotor functions. [J Clin Invest] Abstract | Press Release Antitumor Activity from Antigen-Specific CD8 T Cells Generated In Vivo from Genetically Engineered Human Hematopoietic Stem Cells Here, investigators present the generation of functional tumor-specific human T cells in vivo from genetically modified human hematopoietic stem cells using a human/mouse chimera model. [Proc Natl Acad Sci USA] Abstract | Press Release Human Artificial Chromosome (HAC) Vector with a Conditional Centromere for Correction of Genetic Deficiencies in Human Cells Here, scientists demonstrate the utility of HAC, which has a unique gene acceptor site, for delivery of full-length genes and correction of genetic deficiencies in human cells. [Proc Natl Acad Sci USA] Abstract | Press Release Epirubicin Potentiates rAAV2/5-Mediated TRAIL Expression in Fibroblast-Like Synoviocytes and Augments the Anti-Arthritis Effects of rAAV2/5-TRAIL This study was undertaken to investigate the functions of recombinant adeno-associated virus (rAAV2/5)- tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) in fibroblast-like synoviocytes and arthritic mice. [Arthritis Rheum] Abstract Engineered Cartilage via Self-Assembled hMSC Sheets with Incorporated Biodegradable Gelatin Microspheres Releasing Transforming Growth Factor-β1 Researchers have engineered a system of self-assembled, microsphere-incorporated human mesenchymal stem cell (hMSC) sheets capable of forming cartilage in the presence of exogenous transforming growth factor β1 (TGF-β1) or with TGF-β1 released from incorporated microspheres. [J Control Release] Abstract | Press Release Efficient Reprogramming of Human Cord Blood CD34+ Cells into Induced Pluripotent Stem Cells with OCT4 and SOX2 Alone Here, investigators show that the lentiviral expression of OCT4 together with SOX2 driven by a strong spleen focus-forming virus promoter in a single vector can convert 2% of cord blood CD34+ cells into induced pluripotent stem cells without additional reprogramming factors. [Mol Ther] Abstract Successful Gene Therapy In Utero for Lethal Murine Hypophosphatasia In the present study, researchers investigated the feasibility of fetal gene therapy using the lethal hypophosphatasia model mice. [Hum Gene Ther] Abstract Rational Bioprocess Design for Human Pluripotent Stem Cell Expansion and Endoderm Differentiation Based on Cellular Dynamics Scientists present a predictive bioprocess design strategy employing cell- and molecular-level analysis of rate-limiting steps in human pluripotent stem cell expansion and differentiation, and apply it to produce definitive endoderm progenitors using a scalable directed-differentiation technology. [Biotechnol Bioeng] Abstract | Press Release Charge Inversion, Condensation and Decondensation of DNA and Polystyrene Sulfonate by Polyethylenimine Researchers studied the formation and structure of stable electrostatic complexes between polyanions (DNA and poly(styrene-sulfonate)) and linear polyethylenimine. [Eur Phys J E Soft Matter] Abstract | Press Release
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