Multiplex Genome Engineering Using CRISPR/Cas Systems Researchers engineered two different type II CRISPR (clustered regularly interspaced short palindromic repeats) systems and demonstrated that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells. [Science] Abstract | Press Release Regeneration of Human Tumor Antigen-Specific T Cells from iPSCs Derived from Mature CD8+ T Cells Researchers established iPSCs from mature cytotoxic T cells specific for the melanoma epitope MART-1. When cocultured with OP9/DLL1 cells, these iPSCs efficiently generated TCRβ+CD4+CD8+ double positive cells expressing a T cell receptor specific for the MART-1 epitope. Stimulation of the CD8+ T cells with MART-1 antigen-presenting cells led to the secretion of IFNγ, demonstrating their specific reactivity. [Cell Stem Cell] Abstract | Graphical Abstract | Press Release Generation of Rejuvenated Antigen-Specific T Cells by Reprogramming to Pluripotency and Redifferentiation Investigators reprogrammed clonally expanded antigen-specific CD8+ T cells from an HIV-1-infected patient to pluripotency. The T cell-derived induced pluripotent stem cells were then redifferentiated into CD8+ T cells that had a high proliferative capacity and elongated telomeres. These rejuvenated cells possessed antigen-specific killing activity and exhibited T cell receptor gene-rearrangement patterns identical to those of the original T cell clone from the patient. [Cell Stem Cell] Abstract | Graphical Abstract | Press Release Multimodal Actions of Neural Stem Cells in a Mouse Model of ALS: A Meta-Analysis Investigators tested the hypothesis that transplanted, undifferentiated multipotent migratory neural stem cells may help to ameliorate an array of pathological mechanisms in the SOD1G93A transgenic mouse model of amyotrophic lateral sclerosis (ALS). [Sci Transl Med] Abstract | Press Release Umbilical Cord Blood Therapy Potentiated with Erythropoietin for Children with Cerebral Palsy: A Double-Blind, Randomized, Placebo-Controlled Trial Investigators assessed the safety and efficacy of allogeneic umbilical cord blood potentiated with recombinant human erythropoietin in children with cerebral palsy. [Stem Cells] Abstract | Full Article Bone Marrow Transplantation in Hindlimb Muscles of Motoneuron Degenerative Mice Reduces Neuronal Death and Improves Motor Function Scientists transplanted bone marrow cells into a mouse model of motoneuron degeneration, with the particularity of placing the cells in the hindlimb muscles rather than in the spinal cord where neuronal degeneration occurs. To this end, they analyzed the possibility for the transplanted cells to increase the survival rate of the spinal cord motoneurons by axonal-guided retrograde neurotrophism. As a result, the mice significantly improved their motor functions. [Stem Cells Dev] Abstract Stem Cell Therapy Remediates Reconstruction of the Craniofacial Skeleton after Radiation Therapy This study utilized transplanted bone marrow stromal cells (BMSCs) as a cellular replacement therapy to remedy radiation induced injury and restore impaired new bone formation during distraction osteogenesis (DO). BMSC therapy brought about the successful generation of new bone and significantly improved both the rate and quality of bony union of irradiated, distracted murine mandibles to the level of non-irradiated DO animals. [Stem Cells Dev] Abstract PEP-1-CAT-Transduced Mesenchymal Stem Cells Acquire an Enhanced Viability and Promote Ischemia-Induced Angiogenesis Researchers investigated the role of PEP-1-CAT transduction in mesenchymal stem cells’ survival and its effect on ischemia-induced angiogenesis. [PLoS One] Full Article Cell Therapy with Bone Marrow Stromal Cells after Intracerebral Hemorrhage: Impact of Platelet-Rich Plasma Scaffolds Cell therapy using bone marrow stromal cells (BMSCs) has been considered a promising strategy for neurologic sequelae after intracerebral hemorrhage. Investigators demonstrated neurologic improvement after BMSC transplantation and significantly better functional improvement for the group of animals that received BMSCs in the platelet-rich plasma-derived scaffold compared with the group that received BMSCs in saline. [Cytotherapy] Abstract | Full Article Injection of Vessel-Derived Stem Cells Prevents Dilated Cardiomyopathy and Promotes Angiogenesis and Endogenous Cardiac Stem Cell Proliferation in mdx/utrn−/− but Not Aged mdx Mouse Models for Duchenne Muscular Dystrophy The authors hypothesized that mesoangioblast stem cells (aorta-derived mesoangioblasts) would restore dystrophin and alleviate or prevent dilated cardiomyopathy in animal models of Duchenne muscular dystrophy. [Stem Cells Transl Med] Abstract
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