Characterization of Pluripotent Stem Cells This protocol provides a description of all the immunodetection procedures necessary to characterize mouse and human stem cell lines in different circumstances. [Nat Protoc] Full Article Homing of Regulatory T Cells to Human Skin Is Important for the Prevention of Alloimmune-Mediated Pathology in an In Vivo Cellular Therapy Model To test the hypothesis that different populations of regulatory T cells (Treg) display distinct efficacy in vivo based on their expression of tissue-specific homing molecules, the authors evaluated the activity of human Treg derived from two disparate sources in a model of human skin transplantation. Treg were derived from adult blood or cord blood and expanded in vitro. [PLoS One] Full Article HHV-6 Cell Receptor CD46 Expression on Various Cell Subsets of Six Blood and Graft Sources: A Prospective Series Investigators prospectively compared the human herpes virus (HHV-6) cell receptor CD46 expression on various cell subsets of three freshly harvested blood sources on one hand and of three graft sources on the other hand. 52 samples were used for the purpose of this study. They were issued from peripheral blood (PB), G-CSF mobilized PB, cord blood (CB), unmanipulated bone marrow, leukapheresis product and thawed CB graft. [J Clin Virol] Abstract Outcome of Respiratory Syncytial Virus Lower Respiratory Tract Disease in Hematopoietic Cell Transplant Recipients Receiving Aerosolized Ribavirin: Significance of Stem Cell Source and Oxygen Requirement Researchers evaluated the effect of transplant and treatment factors on overall survival, mortality from respiratory failure, and pulmonary function among 82 hematopoietic cell transplantation recipients who had respiratory syncytial virus lower respiratory tract disease. All patients received aerosolized ribavirin. In multivariable analyses, only the use of marrow or cord blood as graft source and oxygen requirement remained independently associated with overall mortality and death due to respiratory failure. [Biol Blood Marrow Transplant] Abstract Some Viral miRNAs Were Up-Regulated in Megakaryocytes Incubated with ITP Plasma Researchers investigated whether viral microRNAs (miRNAs) are involved in the pathogenesis of immune thrombocytopenia (ITP) for example by modulating the production of megakaryocytes. Plasma samples from adult patients with acute ITP or healthy volunteers were added to the culture system wherein umbilical cord blood mononuclear cells were proliferating into megakaryocytes. [Eur J Haematol] Abstract DNA Damage Response in CD133+ Stem/Progenitor Cells from Umbilical Cord Blood: Low Level of Endogenous Foci and High Recruitment of Tumor Suppressor p53 Binding Protein 1 Scientists examined the repair of radiation-induced DNA double-strand breaks in CD133+ umbilical cord blood cells. [Int J Radiat Biol] Abstract Radiosensitivity of Human Hematopoietic Stem/Progenitor Cells The radiosensitivity of human lineage-committed myeloid hematopoietic stem/progenitor cells (HSPCs) was studied. The present results suggest that there are large individual differences in the surviving fraction of HSPCs contained in both adult peripheral blood and fetus/neonate cord blood. [J Radiol Prot] Abstract | Full Article Expression of Scleraxis and Tenascin C in Equine Adipose and Umbilical Cord Blood Derived Stem Cells Is Dependent Upon Substrata and FGF Supplementation Investigators aimed to determine the expression of scleraxis and tenascin C, two markers of tenocytes, in adipose and umbilical cord blood stem cells during culture on various substrata and in response to fibroblast growth factor (FGF) treatment. [Cytotechnology] Abstract |