Myeloid-Derived Suppressor Cells Suppress Antitumor Immune Responses through IDO Expression and Correlate with Lymph Node Metastasis in Patients with Breast Cancer Because IDO plays a pivotal role in immune tolerance via suppressing T cell function, the aim of this study was to investigate the expression of IDO in myeloid-derived suppressor cells (MDSCs) in breast cancer and its role in MDSC-mediated inhibition of immune surveillance. CD33+ progenitors isolated from healthy donors’ umbilical cord blood were cocultured with breast cancer cell line MDA-MB-231 cells to induce MDSCs. [J Immunol] Abstract Both the Nature of KIR3DL1 Alleles and the KIR3DL1/S1 Allele Combination Affect the KIR3DL1 NK-Cell Repertoire in the French Population Researchers investigated the KIR3DL1+ NK-cell repertoire, taking into account the allelic KIR3DL1/S1 polymorphism, KIR3DL1 phenotype, and function. The higher expression of KIR3DL1 observed on cord blood NK cells suggests the expression of the functional KIR3DL1*004 receptors. [Eur J Immunol] Abstract Graft-versus-Host Disease after Double-Unit Cord Blood Transplantation Has Unique Features and an Association with Engrafting Unit-Recipient HLA-Match Manifestations and risk factors of graft-versus-host disease after double-unit cord blood transplantation (DCBT) are not firmly established. The authors evaluated 115 DCBT recipients transplanted for hematologic malignancies with myeloablative or non-myeloablative conditioning and calcineurin-inhibitor/mycophenolate mofetil immunosuppression. [Biol Blood Marrow Transplant] Abstract Human Endothelial Colony-Forming Cells Expanded with an Improved Protocol Are a Useful Endothelial Cell Source for Scaffold-Based Tissue Engineering A bottleneck in artificial vascularization strategies is to obtain sufficient amounts of endothelial cells, as they can be harvested only in small quantities directly from human tissues. Thus, protocols are required to expand appropriate cells in sufficient amounts without interfering with their capability to settle on scaffold materials and to initiate vessel formation. Researchers analyzed whether umbilical cord blood-derived endothelial colony-forming cells fulfill these requirements. [J Tissue Eng Regen Med] Abstract A Novel Method of CD34+ Cell Separation from Umbilical Cord Blood Umbilical cord blood is rich in the heavily glycosylated CD34 antigen-bearing hematopoietic stem cells that are valuable for transplantation therapy of malignant and nonmalignant disease. CD34+ cell yields of mononuclear cells (UCMCs) isolated by anti-CD34 monoclonal antibody on immunomagnetic particles are insufficient to treat adults. The authors fractionated UCMCs by physicochemical charge-based methods. [Transfusion] Abstract Efficient Expansion of Mesenchymal Stromal Cells in a Disposable Fixed Bed Culture System Investigators evaluated the expansion of cord blood-derived mesenchymal stromal cells in a disposable fixed bed culture system. [Biotechnol Prog] Abstract Cryopreserved Cord Blood Progenitors and Their Cell Adhesion Molecules Are Increased by Coculture with Osteoblasts and Parathyroid Hormone Scientists evaluated whether human parathyroid hormone could increase the number of primitive hematopoietic stem cells with adhesion molecules in cryopreserved cord blood. [J Pediatr Hematol Oncol] Abstract |