Dermal Cell News Volume 1.05 | Jun 15 2015

Generation of Pure Lymphatic Endothelial Cells from Human Pluripotent Stem Cells and Their Therapeutic Effects on Wound Repair
Scientists report that lymphatic endothelial cells could be selectively isolated from differentiating human pluripotent stem cells, and that these cells were potent for lymphatic vessel formation in vivo and wound healing. [Sci Rep] Full Article

Transcription Factor Paired Box 6 Controls Limbal Stem Cell Lineage in Development and Disease
By using an in vitro feeder-free culture system, scientists showed that PAX6 knockdown in limbal stem cells led to an upregulation of skin epidermal specific keratins, concomitant with differentiation to a skin fate. [J Biol Chem] Abstract | Full Article

Exogenous Tryptophan Promotes Cutaneous Wound Healing of Chronically Stressed Mice through Inhibition of TNF-α and IDO Activation
To investigate the role of indoleamine 2, 3-dioxygenase (IDO) expression and tryptophan administration in adverse effects of stress on cutaneous wound healing, mice were submitted to chronic restraint stress and treated with tryptophan daily until euthanasia. [PLoS One] Full Article

SOX 1, Contrary to SOX 2, Suppresses Proliferation, Migration, and Invasion in Human Laryngeal Squamous Cell Carcinoma by Inhibiting the Wnt/β-Catenin Pathway
Scientists found that overexpression of Sex-determining region Y (SRY)-box protein 1 (SOX 1) could significantly inhibit proliferation and promote apoptosis in Tu212 cells. Additionally, overexpression of SOX 1 suppressed the migration and invasion potential of Tu212 cells via regulating Wnt/β-catenin pathway. [Tumour Biol] Abstract

Keratinocyte Nicotinic Acetylcholine Receptor Activation Modulates Early TLR2-Mediated Wound Healing Responses
The authors demonstrated that keratinocyte α7 nicotinic acetylcholine receptors (nAChR) activation dampened toll-like receptor 2 (TLR2)-mediated migration and pro-inflammatory cytokine and antimicrobial peptide production, which was restored by a α7-selective nAChR antagonist. The mechanism of this response occurred by blocking the NF-κB and Erk1/2 pathway during early and late wound healing. [Int Immunopharmacol] Abstract

SKIN CANCERS & DISORDERS

Pluripotency Factor Nanog Is Tumorigenic by Deregulating DNA Damage Response in Somatic Cells
The expression of Nanog in mouse skin activated tumor suppressor p53, leading to the differentiation of epidermal stem cells. In the absence of p53, Nanog induced spontaneous squamous cell carcinoma, identifying a novel role of Nanog in tumorigenesis. [Oncogene] Abstract

Fibronectin Induction Abrogates the BRAF Inhibitor Response of BRAF V600E/PTEN-Null Melanoma Cells
Investigators used mass spectrometry-based phosphoproteomics to determine how BRAF inhibition remodeled the signaling network of melanoma cell lines that were BRAF mutant and PTEN null. [Oncogene] Abstract

Lin28B Promotes Melanoma Growth by Mediating a MicroRNA Regulatory Circuit
Researchers showed that Lin28B was aberrantly expressed in a large proportion of melanoma patients and was functionally required for melanoma progression. They further demonstrated the involvement of let-7-dependent mechanism downstream of Lin28B, resulting in the activation of TGF-β signaling cascade. [Carcinogenesis] Abstract

LASP1, a Newly Identified Melanocytic Protein with a Possible Role in Melanin Release, but Not in Melanoma Progression
Researchers investigated the expression and function of LIM and SH3 protein 1 (LASP1) in normal skin, melanocytic nevi and malignant melanoma. In normal skin, a distinct LASP1 expression is visible only in the basal epidermal layer while in nevi LASP1 protein is detected in all melanocytes. [PLoS One] Full Article

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STEMCELL Technologies Named Company of the Year by BIOTECanada
STEMCELL Technologies Inc. will be presented with the Biotech Company of the Year award by BIOTECanada at this year’s BIO International Convention in Philadelphia. [BIOTECanada] Press Release

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Postdoctoral Researcher – Ocular Melanoma (Ohio State University)

Postdoctoral Fellow – Computational Biology and Bioinformatics in Melanoma (Icahn School of Medicine at Mount Sinai)

Assistant Professor – Dermatology (University of California, Davis)

Postdoctoral Fellow – Stem Cell Niche Function (Icahn School of Medicine at Mount Sinai)

Assistant Professor – Instructive Biomaterials Engineering (Maastricht University)

Professor – Dermatology (University of Wisconsin – Madison)

Chair of the Department of Dermatology (University of California, Davis)

Postdoctoral Positions – Molecular Cancer Biology (University of Pennsylvania)

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