Dermal Cell News Volume 4.20 | Jun 11 2018

TOP STORY

PELI1 Selectively Targets Kinase-Active RIP3 for Ubiquitylation-Dependent Proteasomal Degradation

Upregulated receptor-interacting protein kinase-3 (RIP3) expression in keratinocytes from toxic epidermal necrolysis (TEN) patients correlated with low expression of pellino E3 ubiquitin protein ligase 1 (PELI1), suggesting that loss of PELI1 may play a role in the pathogenesis of TEN. [Mol Cell] Abstract | Graphical Abstract

(Ranked by impact factor of the journal)

DERMAL STEM CELLS & TISSUE REGENERATION

Myosin VI and Branched Actin Filaments Mediate Membrane Constriction and Fission of Melanosomal Tubule Carriers

Investigators showed that myosin VI, together with branched actin filaments, constricted the membrane of tubular carriers that are then released from melanosomes, the pigment containing lysosome-related organelles of melanocytes. Myosin VI motor activity mediated severing by constricting the neck of the tubule at specific melanosomal subdomains. [J Cell Biol] Abstract

miR-20a-3p Regulates TGF-β1/Survivin Pathway to Affect Keratinocytes Proliferation and Apoptosis by Targeting SFMBT1 In Vitro

Researchers showed that overexpression of miR-20a-3p in HaCaT cells suppressed proliferation and induced apoptosis while its knockdown promoted cell proliferation and reduced cell apoptosis. SFMBT1 was identified as the direct target of miR-20a-3p. SFMBT1 knockdown was demonstrated to inhibit cell growth and induced apoptosis, which was consistent with the function of miR-20a-3p upregulation in HaCaT cells. [Cell Signal] Abstract

Keratinocyte Motility Is Affected by UVA Radiation—A Comparison between Normal and Dysplastic Cells

Under UVA exposure, HaCaT and DOK cell’s behavior differed in terms of movement and proliferation. Dysplastic keratinocytes showed more sensitivity to UVA, exhibiting transient deficiencies in directionality of movement and a delay in re-coating the denuded area. The actin cytoskeleton displayed a cortical organization immediately after irradiation, in both cell lines, similar to mock-irradiated cells. [Int J Mol Sci] Full Article

SKIN CANCERS & DISORDERS

PHIP as a Therapeutic Target for Driver-Negative Subtypes of Melanoma, Breast, and Lung Cancer

Investigators showed that pleckstrin homology domain-interacting protein (PHIP) promoted the progression of “driver-negative” tumors. Suppression of PHIP expression significantly inhibited both tumor cell proliferation and invasion, coordinately suppressing phosphorylated AKT, cyclin D1, and talin1 expression in all three tumor types. [Proc Natl Acad Sci USA] Abstract

RIP1 Protects Melanoma Cells from Apoptosis Induced by BRAF/MEK Inhibitors

Scientists reported that receptor-interacting protein kinase 1 (RIP1) was upregulated and contributed to both intrinsic and acquired resistance of melanoma cells to BRAF/MEK inhibitors through activation of NF-κB. Snail1-mediated suppression of CYLD played a crucial role in promoting RIP1 expression upon ERK activation, particularly, in melanoma cells with acquired resistance to BRAF inhibitors. [Cell Death Dis] Full Article

Syringic Acid Prevents Skin Carcinogenesis via Regulation of NoX and EGFR Signaling

Treatment of human epidermal keratinocytes cells with syringic acid resulted in the suppression of UVB-induced cyclooxygenase-2, matrix metalloproteinase-1, and prostaglandin E2 expression as well as activator protein-1 activity. Syringic acid inhibited the UVB-induced phosphorylation of mitogen-activated protein kinases and Akt signaling pathways as well as epidermal growth factor receptor (EGFR). [Biochem Pharmacol] Abstract | Graphical Abstract

C10orf99 Contributes to the Development of Psoriasis by Promoting the Proliferation of Keratinocytes

The authors demonstrated that C10orf99 promoted keratinocyte proliferation by facilitating the G1/S transition, and the pro-proliferation effect of C10orf99 was associated with the activation of the ERK1/2 and NF-κB but not the AKT pathways. Local depletion of C10orf99 by lentiviral vectors expressing C10orf99 shRNA effectively ameliorated IMQ-induced dermatitis. [Sci Rep] Full Article

Temperature-Dependent Cell Death Patterns Induced by Functionalized Gold Nanoparticle Photothermal Therapy in Melanoma Cells

Researchers synthesized gold nanorods with a targeting adaptor FA (GNRs-FA), which achieved high efficacy of targeted delivery to melanoma cells. They demonstrated that photothermal therapy (PTT), precipitated by GNRs-FA under the induction of near-infrared laser, was temperature-dependent. Photothermal killing of melanoma cells showed different patterns of cell death depending on varying temperature in PTT. [Sci Rep] Full Article

Increased Expression of IL-33 in Rosacea Skin and UVB-Irradiated and LL-37-Treated HaCaT Cells

Investigators explored the role played by IL-33 in the pathophysiology of rosacea. In vitro, UVB and LL-37 synergistically increased mRNAs expression of proinflammatory cytokines, especially IL-33 and IL-1β. IL-33 protein release was also synergistically increased by LL-37 and UVB treatment. LL-37 and IL-33 stimulated VEGF mRNA expression and VEGF release from HaCaT cells. [Exp Dermatol] Abstract

Identification and Functional Analysis of Inflammation-Related miRNAs in Skin Wound Repair

The authors summarize recent advances of miRNA studies in skin wound healing, introduce their miRNA purification system using an immunoprecipitation assay method, and discuss the function of miR‐142 in skin wound healing. [Dev Growth Differ] Abstract

Visit our reviews page to see a complete list of reviews in the dermal cell research field.

Dual-Purpose Wound Dressing Supported by $2 Million NIH Grant

Development of wound dressings made of semi-synthetic biomaterials will be the focus of a five-year, $2 million grant from the National Institutes of Health’s National Institute of Arthritis and Musculoskeletal and Skin Diseases. Once developed, this new kind of wound dressing would promote faster and more complete healing for the millions of people who suffer from skin wounds. [The Pennsylvania State University] Press Release

HedgePath Pharmaceuticals Granted Meeting with FDA to Discuss SUBA BCCNS Trial Data and Regulatory Pathway

HedgePath Pharmaceuticals, Inc. (HPPI) announced that the FDA, has granted HPPI a face-to-face meeting to discuss the results obtained in HPPI’s open label, Phase II(b) clinical trial testing SUBA^™-Itraconazole in patients with Basal Cell Carcinoma Nevus Syndrome (BCCNS), also known as Gorlin Syndrome. [Hedgepath Pharmaceuticals, Inc.] Press Release

More than 5,000 Patients Enrolled in Corrona^® Psoriasis Registry

Created to help researchers compare the safety and effectiveness of approved psoriasis therapies, the Corrona Psoriasis Registry is the largest, independent North American registry of psoriasis patients from 175 sites across the US and Canada. [National Psoriasis Foundation (PR Newswire Association LLC.)] Press Release

China Introduces Sweeping Reforms to Crack Down on Academic Misconduct

China is getting tough on scientific misconduct. The country’s most powerful bodies, the Chinese Communist Party and the State Council, introduced a raft of reforms on 30 May aimed at improving integrity across the research spectrum, from funding and job applications to peer-review and publications. [Nature News] Editorial

More Restrictive U.S. Policy on Chinese Graduate Student Visas Raises Alarm

Reversing yet another policy of the previous administration, the U.S. Department of State began applying tougher restrictions on some Chinese graduate students. The new policy shortens from five years to one year the duration of visas for those planning to study aviation, robotics, and advanced manufacturing. [ScienceInsider] Editorial

Leading Salk Scientist Resigns after Allegations of Harassment

The prominent cancer biologist Inder Verma unconditionally resigned from the Salk Institute for Biological Studies in San Diego, California, June 6, and the research institute’s board of trustees have voted unanimously to accept his resignation. [ScienceInsider] Editorial

EU to World: Join Our €100-Billion Research Program

The European Commission has outlined how it plans to spend the biggest research and innovation budget in its history. In a document that sets out plans for its next major science-funding program, the Commission also confirmed new rules that will, for the first time, allow any country in the world — including a post-Brexit United Kingdom — to take part. [Nature News] Editorial

Go for Launch: A Former Astronaut Becomes Spain’s Science Minister

Spain has a ministry of science again—and none other than the first Spanish astronaut is leading it. The new transition government led by socialist Pedro Sánchez announced that Pedro Duque, who visited space twice, will be at the helm of the newly created Ministry for Science, Innovation, and Universities. The announcement was cheered by the Spanish scientific community, which has long suffered from declining budgets and bureaucratic hurdles. [ScienceInsider] Editorial

Chile, Keen to Become a Knowledge Society, Creates a Ministry of Science

Scientists in Chile have welcomed a decision by Congress to create a science ministry. Many researchers hope that a dedicated ministry will give science more prominence and better-coordinated policies—provided the ministry’s budget matches the government’s ambitions to “bring Chile towards an information and knowledge society,” as Gonzalo Blumel, the country’s minister secretary-general of the presidency, put it in a statement issued after the 31 May vote. [ScienceInsider] Editorial

NEW 30^th EORTC-NCI-AACR Symposium
November 13-16, 2018
Dublin, Ireland

Visit our events page to see a complete list of events in the community.

NEW Postdoctoral Research Fellow – Melanoma Research (Edith Cowan University)

Postdoctoral Positions – Cell Competition in Fly, Mouse and Human In Vitro Models (University of Bristol)

Assistant, Associate or Full Professor – Regenerative Medicine (University of San Diego)

Postdoctoral Fellow – Skin Stem Cells & Wound Healing (Johns Hopkins University)

Postdoctoral Fellow – Cancer Signaling & Trascription (The Francis Crick Institute)

Associate Professor or Professor – Skin Cancer (The University of Texas M.D. Anderson Cancer Center)

Postdoctoral Fellow – Psoriasis Research (Case Western Reserve University)

Postdoctoral Fellow – Skin Cancer Biology (UT Southwestern Medical Center)

Postdoctoral Researcher – Melanoma (Ghent University)

Faculty Position – Developmental Biology and Regenerative Medicine (Seattle Children’s Research Institute)

PhD or Postdoctoral Position – Tissue Repair, Stem Cell Fate and Tumor Heterogeneity (Université Libre de Bruxelles)

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