Dermal Cell News Volume 5.36 | Oct 28 2019

TOP STORY

Senescent Human Melanocytes Drive Skin Aging via Paracrine Telomere Dysfunction

Scientists showed that melanocytes were the only epidermal cell type to express the senescence marker p16^INK4A during human skin aging. Aged melanocytes also displayed additional markers of senescence such as reduced HMGB1 and dysfunctional telomeres, without detectable telomere shortening. [EMBO J] Abstract

(Ranked by impact factor of the journal)

DERMAL STEM CELLS & TISSUE REGENERATION

Autologous Micrograft Accelerates Endogenous Wound Healing Response through ERK-Induced Cell Migration

Investigators showed that autologous micrograft (AMG) improved skin re-epithelialization by accelerating the migration of fibroblasts and keratinocytes. More specifically, AMG-treated wounds showed improvement of indispensable events associated with successful wound healing such as granulation tissue formation, organized collagen content, and newly formed blood vessels. [Cell Death Differ] Abstract

Millimeter-Wave Pulsed Heating In Vitro: Cell Mortality and Heat Shock Response

Scientists evaluated apoptotic and heat shock responses of melanoma cells exposed in vitro to continuous or pulsed-wave amplitude-modulated MMW at 58.4 GHz with the same average temperature rise. Using an ad hoc exposure system, they generated 90 minute pulse trains with 1.5 second pulse duration, period of 20 seconds, amplitude of 10^°C, and steady-state temperature at the level of cells of 49.2^°C. [Sci Rep] Full Article

Nitrogen Mustard Prevents Transport of Fra-1 into the Nucleus to Promote C-Fos- and FosB-Dependent IL-8 Induction in Injured Mouse Epidermis

In nitrogen-mustard-exposed mouse skin, scientists found p-ERK activation increased Fra-1 and FosB. Unlike the nucleus location of c-Fos and FosB, Fra-1 and Fra-2 were mainly expressed in the cytoplasm. In nitrogen-mustard-exposed cultured immortalized human keratinocytes, Fra-1 in the nucleus functioned as an inhibitor of inflammatory cytokine interleukin IL-8. [Toxicol Lett] Abstract

Anti-inflammatory Effect of Epidermal Growth Factor Conjugated Silk Fibroin Immobilized Polyurethane Ameliorates Diabetic Burn Wound Healing

Effective silk fibroin dosage was determined and incorporated according to minimum inhibitory concentrations against wound associated bacterial strains while fabricating scaffold. Dermal fibroblast NIH3T3 cells were seeded on epidermal growth factor treated and untreated polyurethane/silk fibroin scaffolds, fabricated by blending and immobilization techniques. [Int J Biol Macromol] Abstract | Graphical Abstract

Boehmite Enhances Hair Follicle Growth via Stimulation of Dermal Papilla Cells by Upregulating β-Catenin Signaling

Investigators evaluated human dermal papilla cells viability, alkaline phosphatase activity, protein expression, and vascular endothelial growth factor secretion in vitro and assessed the anagen-promoting effects of boehmite via gross observation and histological analysis in a mouse model. [Exp Dermatol] Abstract

SKIN CANCERS & DISORDERS

GABA-A Receptor and Mitochondrial TSPO Signaling Act in Parallel to Regulate Melanocyte Stem Cell Quiescence in Larval Zebrafish

Through pharmacological and genetic approaches, the authors showed that GABA-A acts through calcium signaling to maintain melanocyte stem cell quiescence. Unexpectedly, the authors identified translocator protein (TSPO), a mitochondrial membrane associated protein that regulated mitochondrial function and metabolic homeostasis, as a parallel regulator of melanocyte stem cell quiescence. [Pigment Cell Melanoma Res] Abstract

Expression Level of EGFR and MET Receptors Regulates Invasiveness of Melanoma Cells

Researchers analyzed direct involvement of these receptors in the invasion of melanoma cells inducing EGFR and MET up- and down-regulations in examined cells. Results were acquired with assays evaluating cell migration and invasion (scratch wound assay, Transwell filter-based method and single-cell tracking). [J Cell Mol Med] Full Article

Small Extracellular Vesicles Convey the Stress-Induced Adaptive Responses of Melanoma Cells

Investigators compared B16F1 cell-derived small extracellular vesicles (sEVs) released under normal and stress conditions, including cytostatic, heat and oxidative stress. The miRNome and proteome showed substantial differences across sEV groups and bioinformatics analysis of the obtained data by the Ingenuity Pathway Analysis also revealed significant functional differences. [Sci Rep] Full Article

RNF6 Promotes Myeloma Cell Proliferation and Survival by Inducing Glucocorticoid Receptor Polyubiquitination

Researchers first showed that the expression levels of RNF6 in multiple myeloma were significantly elevated compared with the bone marrow cells of healthy donors. Overexpression of RNF6 in LP1 and PRMI-8266 multiple myeloma cell lines promoted cell proliferation, whereas knockdown of RNF6 led to apoptosis of multiple myeloma cells. [Acta Pharmacol Sin] Abstract

Subscribe to one of our other 19 science newsletters such as Extracellular Matrix News & Cancer Stem Cell News.

New and Evolving Concepts of Melanocytic Nevi and Melanocytomas

Recently, many of the mutations that initiate nevus formation have been identified and specific sets of mutations are found in different subtypes of nevi. The author provides an update on the broad spectrum of melanocytic nevi and melanocytomas and outlines their key histopathologic and genetic features. [Mod Pathol] Abstract

Immune-Competent In Vitro Co-culture Models as an Approach for Skin Sensitization Assessment

Significant progress has been made over the past decades to develop new technologies of data generation concurrently with novel approaches to improve the models especially by the use of co-culture. Scientists address the technical aspects to take into consideration when co-culturing depending on the desired objective and describes the different keratinocytes and dendritic cells co-cultures developed in 2D and 3D. [Toxicol In Vitro] Abstract

Visit our reviews page to see a complete list of reviews in the dermal cell research field.

MOR106 Clinical Development in Atopic Dermatitis Stopped for Futility

MorphoSys AG and Galapagos NV announced the end of the clinical development program of MOR106 in atopic dermatitis. The joint decision of all three involved parties, Galapagos NV, MorphoSys AG and Novartis Pharma AG, was based on an interim analysis for futility that was performed in the Phase II IGUANA trial. [MorphoSys AG] Press Release

DermTech Receives CPT^® Proprietary Laboratory Assay Code from the American Medical Association

DermTech, Inc. announced the American Medical Association has issued a Proprietary Laboratory Analyses (PLA) Common Procedural Terminology (CPT^®) Code for the DermTech Pigmented Lesion Assay. This PLA code was published online with an effective date of July 1, 2019 and is included in the CPT 2020 publication. [DermTech, Inc. (GlobeNewswire, Inc.)] Press Release

UCL to Phase Out Single-Use Plastics, Including Pipette Tips

University College London made a series of pledges last week to cut down on plastic waste, help mitigate climate change, and make its research and teaching facilities more environmentally sustainable, according to a press release issued on October 16. If all goes to plan, UCL will divest from fossil fuel-producing companies by the end of this year, have one and a half football pitches’ worth of green space on its campus within five years, and offer vegetarian options only through its catering and hospitality services by 2030. [TheScientist] Editorial

NEW 10^th International Meeting of the Stem Cell Network North Rhine-Westphalia
June 8-9, 2020
Aachen, Germany

Visit our events page to see a complete list of events in the community.

NEW Postdoctoral Research Scientist – Genetic Hair and Skin Disorders (Columbia University)

NEW Postdoctoral Studentship – Melanoma and Immunotherapy Checkpoint Inhibitors (University of Manchester)

Research Coordinator – Dermatology and Venereology (Karolinska Institutet)

Postdoctoral Fellowship – DNA Damage Repair Pathways in Melanoma Prevention (University of Southern California)

Postdoctoral Training Fellow – Evolutionary Biology in Renal Cancer and Melanoma (The Francis Crick Institute)

Medical Science Liaison – Dermatology (Eli Lilly and Company)

Assistant Professor – Biochemistry (University of Washington)

Postdoctoral Fellowship – Dermatology (Brigham and Women’s Hospital)

Postdoctoral Fellowship – Wound Healing and Tissue Regeneration (Memorial Sloan Kettering Cancer Center)

Postdoctoral Researcher – Cancer Research (Champalimaud Foundation)

Postdoctoral Position – Cancer Immunology (Northwestern University Feinberg School of Medicine)

Faculty Position – Cancer Biology (Children’s Research Institute at UT Southwestern)

Faculty Position – Cancer Biology (Emory University)

Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.

Have we missed an important article or publication in Dermal Cell News? Click here to submit!

Comments or suggestions? Submit your feedback here.