Endothelial Cell News Volume 1.10 | May 16 2016

Activated Stat5 Trafficking via Endothelial Cell-Derived Extracellular Vesicles Controls IL-3 Pro-Angiogenic Paracrine Action
Researchers found that interleukin 3 (IL-3) increases extracellular vesicle (EV) release, which is prevented by IL-3Ralpha blockade. EVs released upon IL-3 stimulation were able to induce pro-angiogenic signals as shown by chromatin immunoprecipitation assay performed on the promoter region of cyclin D1 and tridimensional tube-like structure formation. [Sci Rep] Full Article

Interleukin-33 Stimulates GM-CSF and M-CSF Production by Human Endothelial Cells
The authors investigated a possible influence of interleukin-33 on granulocyte macrophage-colony stimulating factor (GM-CSF) and macrophage-CSF (M–CSF) production by human endothelial cells. [Thromb Haemost] Abstract

Proper Autophagy Is Indispensable for Angiogenesis during Chick Embryo Development
Using HUVECs, investigators revealed that cell viability and migration ability changed with the alteration of cell autophagy exposed to RAPA or 3-MA. Interestingly, tube formation assay showed that HUVECs ability of tube formation altered with the change of Atg5, Atg7 and Atg8 manipulated by the transfection of their corresponding siRNA or plasmids. [Cell Cycle] Abstract

Proteome Changes during Transition from Human Embryonic to Vascular Progenitor Cells
Scientists refined an existing method to generate tight vessels by differentiating human embryonic stem cells in CD34⁺ vascular progenitor cells using chemically defined media and growth conditions. [J Proteome Res] Abstract | Graphical Abstract

Long-Range Enhancers Modulate Foxf1 Transcription in Blood Vessels of Pulmonary Vascular Network
Researchers investigated the lung-specific enhancers of Foxf1 and the Gli binding on the enhancers. They found three evolutionarily conserved Foxf1 enhancers, two of which were long-range enhancers. [Histochem Cell Biol] Abstract

The Influence of Geranylgeraniol on Microvessel Sprouting after Bisphosphonate Substitution in an In Vitro 3D-Angiogenesis Assay
The authors investigated the influence of geranylgeraniol on microvessel sprouting after bisphosphonate-incubation in vitro. Ten experimental set-ups were randomly designed in an in vitro 3D-angiogenesis assay. [Clin Oral Investig] Abstract

A Novel Endothelial-Derived Anti-Inflammatory Activity Significantly Inhibits Spontaneous Choroidal Neovascularisation in a Mouse Model
To elucidate the molecular basis of the therapeutic effects of endothelial cells grown on collagen particles, the anti-inflammatory activity of conditioned medium from these cells was characterized. [Vasc Cell] Full Article

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U.S. FDA Approves Additional Indication for Eisai’s Anticancer Agent Lenvima^® in Combination with Everolimus as Treatment for Advanced Renal Cell Carcinoma
Eisai Co., Ltd. announced that its U.S. subsidiary Eisai Inc. has received approval from the U.S. Food and Drug Administration (FDA) for an additional indication for Eisai’s in-house developed novel anticancer agent Lenvima^® in combination with everolimus for the treatment of patients with advanced renal cell carcinoma following one prior anti-angiogenic therapy. [Eisai Co., Ltd.] Press Release

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NEW Postdoctoral Positions – Modeling Vascular Development and Disease in the Zebrafish (University of Massachusetts)

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