Endothelial Cell News 2.10 March 27, 2017 | |
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TOP STORYA Genome-Wide Analysis of Human Pluripotent Stem Cell-Derived Endothelial Cells in 2D or 3D Culture Investigators defined a protocol for efficiently deriving endothelial cells from human pluripotent stem cells. They used vascular morphogenesis as a model system to understand how synthetic hydrogels influence global biological function compared with common 2D and 3D culture platforms. [Stem Cell Reports] Full Article | Graphical Abstract | |
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PUBLICATIONS(Ranked by impact factor of the journal)Scientists examined various transfection vectors, with regard to increasing the transfection efficiency without disrupting the blood–brain barrier integrity. Lipofectamine 3000™ was the most potent vector compared to polyethylenimine and Turbofect. [Cell Mol Life Sci] Abstract The authors investigated the therapeutic potential of vascular endothelial growth factor isoform-B (VEGF-B) in ischemic stroke using different in vivo and in vitro approaches. They showed that the delayed intranasal administration of VEGF-B reduced neuronal damage and inflammation. [Mol Neurobiol] Abstract Researchers investigated how the presence of endothelial cells in microchannels affects their hydrodynamic resistance and the near-wall motion of red blood cells. They showed that, under their culture conditions, endothelial cells form a confluent layer on all the walls of the circuit and display a glycocalyx that fully lines the lumen of the microchannels. [Sci Rep] Full Article The authors coated decellularized vessels obtained from porcine carotid arteries with poly(ethylmethacrylate-co-diethylaminoethylacrylate) with the purpose of improving endothelial coverage and minimizing platelet attachment while enhancing the mechanical properties of the decellularized vascular scaffolds. The polymer facilitated binding of endothelial cells with high affinity and also induced endothelial cell capillary tube formation. [Sci Rep] Full Article Scientists tested the hypothesis that constitutive endothelial cell-specific activation of TGF-β signaling induces tissue fibrosis and vasculopathy resembling the characteristic fibrotic and vascular alterations of systemic sclerosis. Constitutive TGFβ-1 signaling in endothelial cells resulted in cutaneous and visceral fibrosis with prominent fibrotic involvement of the lungs and severe perivascular and subendothelial fibrosis of small arterioles. [Lab Invest] Abstract Investigators tested the hypothesis that the MCAK contributes to compliance and dimensionality mechanosensing-mediated regulation of microtubule (MT) growth dynamics through a myosin-II-dependent signaling pathway. Their results identified that MCAK promotes fast MT growth speeds in endothelial cells (ECs) cultured on compliant 2D extracellular environment (ECMs), but promotes slow MT growth speeds in ECs cultured on compliant 3D ECMs, and these effects are myosin-II-dependent. [Mol Biol Cell] Abstract | Full Article Scientists investigated the effect of human IL-6, IFN-γ, IL-17, IL-1β, and TNF-α in xenotransplantation using several in vitro models and porcine aortic endothelial cells (PAECs) as target cells. They found that NF-κB and mitogen-activated protein kinases were activated by recombinant human (rh)IL-17, rhIL-1β, and rhTNF-α, while rhIL-6 activated signal transducer and activator of transcription 3 in PAECs. [Xenotransplantation] Abstract Evodiamine Attenuates TGF-β1-Induced Fibroblast Activation and Endothelial to Mesenchymal Transition Researchers investigated the effect of evodiamine on fibroblast activation in cardiac fibroblasts and endothelial to mesenchymal transition in human umbilical vein endothelial cells. Evodiamine-blunted TGF-β1 induced activation of cardiac fibroblast into myofibroblast as assessed by the decreased expressions of α-SMA. [Mol Cell Biochem] Abstract The authors analyzed BMP9 signaling in a co-culture of several types of human primary culture endothelial cells and mural cells. The co-culture activated the Notch pathway in both types of cells in a co-culture- and BMP9-dependent manner. [Biol Open] Full Article | |
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REVIEWST-Lymphocyte Homing: An Underappreciated yet Critical Hurdle for Successful Cancer Immunotherapy Scientists scrutinize evolving knowledge on the molecular determinants of T-effector (Teff) cell navigation into tumors. By integrating recently described, though sporadic information of pivotal adhesive and chemokine homing signatures within the tumor microenvironment with better established paradigms of T-cell trafficking under homeostatic or infectious disease scenarios, they seek to refine currently incomplete models of Teff cell entry into tumor tissue. [Lab Invest] Full Article Kruppel-Like Factors in Endothelial Cell Biology Researchers review the role of Kruppel-like factors in physiological activity and their loss in vascular pathology. [Curr Opin Hematol] Abstract A Short Discourse on Vascular Tissue Engineering The authors discuss past and current achievements in tissue engineering of microvasculature to perfuse organ specific constructs, small vessels for dialysis grafts, and modified synthetic and pediatric large caliber-vessel grafts. [NPJ Regen Med] Full Article Visit our reviews page to see a complete list of reviews in the endothelial cell research field. | |
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INDUSTRY NEWSThe G-BA confirmed the additional benefit of Kisplyx® in combination with everolimus for the treatment of adults with advanced RCC versus everolimus alone. With this decision, the G-BA followed the recommendation of the German Institute for Quality and Efficiency in Health Care thereby also affirming that the data presented by Eisai provided convincing evidence of a clinically meaningful added survival benefit for the combination. [Eisai Limited (PR Newswire Association LLC.)] Press Release Adrenomed AG announced the successful completion of a first-in-human study that demonstrated excellent safety and tolerability of Adrecizumab in healthy volunteers. The monoclonal Adrenomedullin-specific antibody Adrecizumab was developed to selectively support the beneficial effects of Adrenomedullin, an endogenous hormone that maintains endothelial integrity and prevents vascular leakage. [Adrenomed AG] Press Release | |
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POLICY NEWSElection Chaos at Russian Academy of Sciences Academics at Russia’s premier science body have been left shocked and confused after an election that was supposed to determine the new president of the Russian Academy of Sciences was cancelled at the last minute. [Nature News] Editorial NIH Enables Investigators to Include Draft Preprints in Grant Proposals Researchers should feel free to include preprints, or draft manuscripts that haven’t yet been gone through peer review, as part of their applications when they seek funding from the Bethesda, Maryland–based National Institutes of Health (NIH), the agency announced. [ScienceInsider] Editorial Data Check: NSF Sends Congress a Garbled Message on Misconduct Numbers When a senior National Science Foundation (NSF) official told the House of Representatives science committee this month about a “significant rise in the number of substantive allegations” of research misconduct, her testimony set off alarm bells. Legislators from both parties were clearly disturbed by this trend, which had led to three dozen findings of misconduct a year, and asked what the Arlington, Virginia–based NSF was doing about it. [ScienceInsider] Editorial
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EVENTSNEW Mitochondrial Biogenesis and Dynamics in Health, Disease and Aging Visit our events page to see a complete list of events in the community.
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JOB OPPORTUNITIESResearch Associate – Cell Biology (University of Bristol) Doctoral Research Position – Infection Biology (The Leibniz Institute for Natural Product Research) Postdoctoral Position – Bioinformatics (The National Center for Scientific Research) PhD Student – Brain Vascular Biology (ETH Zurich) Postdoctoral Research Position – Retinal Cell Biology (University of California, Davis) Postdoctoral Fellow – Stem Cell Biology (Northwestern University) PhD Position – Molecular Biology (Institute of Molecular Biology) Postdoctoral Fellow – Cardiopulmonary Molecular Biology (Hannover Medical School) Postdoctoral Fellowship – RNA Biology (Goethe University Frankfurt) Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.
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Home Endothelial Cell News Volume 2.10 | Mar 27 2017