Endothelial Cell News 3.10 March 26, 2018 | |
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TOP STORYScientists identified the key cellular mechanisms behind vascular aging and its effects on muscle health and have successfully reversed the process in animals. [Press release from Harvard Medical School discussing online prepublication in Cell] Press Release | Press Release 2 | Abstract | Graphical Abstract | |
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PUBLICATIONS(Ranked by impact factor of the journal)Amino Acid Restriction Triggers Angiogenesis via GCN2/ATF4 Regulation of VEGF and H2S Production The authors identified sulfur amino acid restriction as a proangiogenic trigger, promoting increased VEGF expression, migration and sprouting in endothelial cells in vitro, and increased capillary density in mouse skeletal muscle in vivo via the GCN2/ATF4 amino acid starvation response pathway independent of hypoxia or HIF1α. [Cell] Abstract | Press Release | Graphical Abstract FACS Isolation of Endothelial Cells and Pericytes from Mouse Brain Microregions Investigators describe the detailed procedures for simultaneous isolation of pure populations of endothelial cells and pericytes directly from adult mouse brain microregions using fluorescence-activated cell sorting (FACS) with antibodies against CD31 (endothelial cells) and CD13 (pericytes). [Nat Protoc] Abstract Human iPSC-Derived Endothelial Cells and Microengineered Organ-Chip Enhance Neuronal Development Scientists derived both spinal motor neurons and brain microvascular endothelial cells (BMECs) from human induced pluripotent stem cells and observed increased calcium transient function and chip-specific gene expression in organ-chips compared with 96-well plates. Seeding BMECs in the organ-chip led to vascular-neural interaction and specific gene activation that further enhanced neuronal function and in vivo-like signatures. [Stem Cell Reports] Full Article | Press Release The authors overexpressed follistatin‐like 3 (FSTL3) in induced pluripotent stem cell‐endothelial cells and found that FSTL3 could induce and enhance endothelial features by facilitating β‐catenin nuclear translocation through inhibition of GSK3β activity and induction of Endothelin‐1. The angiogenic potential of FSTL3 was also confirmed both in vitro and in vivo. [Stem Cells] Abstract Human vascular endothelial (VE)-cadherin was a direct target for sunitinib which inhibited its VEGF-induced phosphorylation and cleavage on endothelial monolayer and endothelial cell migration in the 3D model. The tumour cell environment modulated VE-cadherin functions through MMPs and VEGF. [Br J Cancer] Abstract Knock down of microtubule-associated tumor suppressor 1 (MTUS1) disturbed the elongated mitochondrial network and induced the formation of perinuclear clusters of mitochondria. Importantly, mitochondrial motility and fusion were suppressed, whereas generation of reactive oxygen species was increased in MTUS1 knockdown endothelial cells. [FASEB J] Abstract Engineered 3D Vascular and Neuronal Networks in a Microfluidic Platform The authors describe a new 3D microvascular and neuronal network model in a microfluidic platform. Both 3D networks were established by co-culturing human embryonic stem-derived motor neuron spheroids and endothelial cells in microfluidic devices. [Sci Rep] Full Article Researchers aimed to increase the in vivo lifetime of induced pluripotent stem-cell derived endothelial cells by culturing them on electrospun polycaprolactone/gelatin scaffolds, before quantifying the subsequent impact on their proangiogenic function. [Stem Cell Res Ther] Full Article Human Dental Pulp Stromal Cell Conditioned Medium Alters Endothelial Cell Behavior Scientists investigated the effects of conditioned medium derived from human dental pulp stromal cells (hDPSC-CM) on endothelial cell behavior in vitro. hDPSC-CM significantly accelerated the adhesion phases, from sedimentation to attachment and spreading, the proliferation rate and migration of HUVECs as shown in both endpoint assays and real-time cell analysis recordings. [Stem Cell Res Ther] Full Article Investigators compared alternative complement pathway activation and regulation in human glomerular and brain microvascular endothelial cells unstimulated or stimulated by the proinflammatory cytokine, tumor necrosis factor. [J Biol Chem] Abstract | Full Article | |
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REVIEWSShear Stress: An Essential Driver of Endothelial Progenitor Cells Scientists discuss the impact of shear stress on homing, paracrine effects, and differentiation of endothelial progenitor cells (EPCs). Unidirectional laminar shear stress significantly promotes homing of circulating EPCs to endothelial injury sites, induces anti-thrombotic and anti-atherosclerotic phenotype of EPCs, increases their capability to form capillary-like tubes in vitro, and enhances differentiation of EPCs into mature endothelial cells in a dose-dependent manner. [J Mol Cell Cardiol] Abstract Visit our reviews page to see a complete list of reviews in the endothelial cell research field. | |
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INDUSTRY NEWSGenentech announced that the FDA approved the Lucentis® 0.3 mg prefilled syringe (PFS) as a new method of administering the medicine to treat all forms of diabetic retinopathy. The Lucentis 0.3 mg PFS is now the first syringe prefilled with an anti-VEGF agent FDA-approved to treat both diabetic retinopathy and diabetic macular edema. [Genentech] Press Release Eisai Co., Ltd. and Merck announced that the multiple receptor tyrosine kinase inhibitor LENVIMA® has been approved in Japan for unresectable HCC. [Eisai Co.] Abstract | |
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POLICY NEWSFinal 2018 Budget Deal Should Help the National Science Foundation in 2019, Too The 4% increase for the National Science Foundation (NSF) in Alexandria, Virginia, in the 2018 omnibus spending bill hammered out by congressional leaders may be modest next to what its peer science agencies received. But it does offer NSF officials more breathing room to fund some major initiatives starting next year. [ScienceInsider] Editorial Expectations Are High for UKRI, the United Kingdom’s New £6 Billion Research Behemoth Combine U.S. agencies akin to the National Institutes of Health, National Science Foundation, and National Endowment for the Humanities. Toss in some energy and innovation research and fuel it all with the largest boost in R&D spending in recent history. Then put one person in charge. [ScienceInsider] Editorial HIV Researcher Is New Head of US Public-Health Agency HIV researcher Robert Redfield will lead the Centers for Disease Control and Prevention, Secretary of Health and Human Services Alex Azar announced. [Nature News] Editorial
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EVENTSNEW Goodbye Flat Biology: In Vivo Inspired Cancer Biology and Therapy Visit our events page to see a complete list of events in the community.
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JOB OPPORTUNITIESNEW Research Associate – Endothelial Cells (Imperial College London) Research Assistant – Stem Cell Biology in Critical Limb Ischemia (NUI Galway) Postgraduate Research Assistant – Cardiovascular Medicine (University of Oxford) Director – Whitaker Cardiovascular Institute (Boston University) Postdoctoral/Research Associate – Renal and Cardiovascular Research (University of Virginia) Research Associate – Vascular Biology (Case Western Reserve University) Postdoctoral Associate – Bioengineering (University of Pittsburgh) Postdoctoral Research Associate – Vascular Inflammation (University of Illinois) PhD Studentship – Tumor Biology (University College London) Postdoctoral Fellow – Vascular Biology (Weill Cornell Medicine) Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.
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