Endothelial Cell News 3.37 November 5, 2018 | |
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TOP STORYA Mutant-Cell Library for Systematic Analysis of Heparan Sulfate Structure-Function Relationships Investigators report the generation of an heparan sulfate (HS)-mutant mouse lung endothelial cell library by systematic deletion of HS genes expressed in the cell. [Nat Methods] Abstract | |
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PUBLICATIONS(Ranked by impact factor of the journal)Multiple Roles of Lymphatic Vessels in Peripheral Lymph Node Development Using high-resolution imaging, scientists showed that lymphoid tissue inducer (LTi) cells initially transmigrate from veins at lymph node (LN) development sites using gaps in venous mural coverage. This process was independent of lymphatic vasculature, but lymphatic vessels were indispensable for the transport of LTi cells that egressed from blood capillaries elsewhere and served as an essential LN expansion reservoir. [J Exp Med] Abstract | Graphical Abstract Blood-Brain-Barrier Organoids for Investigating the Permeability of CNS Therapeutics Blood-brain barrier (BBB) organoids were obtained following coculture of endothelial cells, pericytes and astrocytes under low-adhesion conditions. These organoids reproduced many features of the BBB, including the expression of tight junctions, molecular transporters and drug efflux pumps, and hence can be used to model drug transport across the BBB. [Nat Protoc] Abstract CD276 knockdown by lentivirus transduction in late endothelial progenitor cells (LEPCs) significantly decreased proliferation and increased apoptosis of LEPCs in vitro. After CD276 silencing, the cell cycle of LEPCs was prone to remain at the G0/G1 phase, and cell migration rates as well as transwell and wound healing migration were decreased. [Stem Cells] Abstract RNA Stability Protein ILF3 Mediates Cytokine-Induced Angiogenesis Proangiogenic stimuli induced interleukin enhancer-binding factor 3 (ILF3) mRNA and protein expression in cultured human coronary artery endothelial cells (hCAECs). Angiogenic indices, including proliferation, migration, and tube formation, were all significantly reduced in hCAECs when ILF3 was knocked down using small interfering RNA, but were significantly increased when ILF3 was overexpressed using adenovirus. [FASEB J] Abstract Untreated extracellular vesicles (EVs) were found to promote endothelial cell migration and proliferation via ERK1/2 and JNK1/2/3 phosphorylation, whereas bortezomib-induced-EVs inhibited these functions. [Angiogenesis] Abstract p17 strongly induced angiogenesis of human brain endothelial cells. p17 concomitantly increased phosphorylation of epidermal growth factor receptor-1 (EGFR1) as well as down-stream intermediates ERK1/2, FAK, PLC-γ and PKC-β whilst an inhibitor peptide of EGFR, blocked cell signaling and angiogenesis. [Lab Invest] Abstract Researchers showed that increased levels of Thy-1 mRNA and protein in endothelial cells were observed at 14-18 hours and 20-28 hours after phorbol-12-myristate-13-acetate (PMA) treatment, respectively. Treatment with PMA for 32 hours induced Thy-1 up-regulation and inhibited capillary-like tube formation and endothelial cell migration. [Sci Rep] Full Article HUVECs were cultured to study the CXC chemokine receptor type 4/stromal cell-derived factor-1α interaction. Targeting of administered exosomes to ischemic kidneys was detected 30 min and 4 hrs after reperfusion. Exosomes increased miR-486-5p levels only in kidneys, within proximal tubules, glomeruli, and endothelial cells. [Sci Rep] Full Article Using HUVECs cultured under physiological chronic low oxygen as a cell model, the authors showed that transfecting cells with adenovirus carrying G protein α subunit 14 (GNA14) complementary DNA increased protein expression of GNA14. GNA14 overexpression blocked fibroblast growth factor 2 (FGF2)‐stimulated endothelial migration, whereas it enhanced endothelial monolayer integrity in response to FGF2. [J Cell Physiol] Abstract Lysyl oxidase‐like 2 (LOXL2) knockdown in endothelial cells resulted in decreased migration and sprouting, and concordantly, LOXL2 overexpression led to an increase in migration and sprouting, independent of its catalytic activity. [J Cell Physiol] Full Article Subscribe to one of our other 19 science newsletters such as Extracellular Matrix News & Cell Therapy News. | |
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REVIEWSPathological Effects of Ionizing Radiation: Endothelial Activation and Dysfunction The authors summarize the current knowledge on endothelial cell activation and dysfunction after ionizing radiation exposure as a central feature preceding the development of cardiovascular diseases. [Cell Mol Life Sci] Full Article Visit our reviews page to see a complete list of reviews in the endothelial cell research field. | |
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INDUSTRY NEWSTufts CTSI Announces Collaboration with Novartis to Foster Innovative Translational Research Leaders from Tufts Clinical and Translational Science Institute (CTSI) and Novartis announced a new collaboration to work together to foster innovative translational research and improve health through the exchange of expertise, resources, and educational opportunities. [Tufts Clinical and Translational Science Institute] Press Release The Lewis Katz School of Medicine at Temple University and Hunter College of the City University of New York have jointly received a five-year, $13.5 million grant from the National Cancer Institute (NCI). [Temple University] Press Release Micro Medical Solutions’ New MicroStent with 120 cm Delivery Catheter Receives CE Mark Approval Micro Medical Solutions announced that it has received CE Mark approval for a 4Fr.-compatible MicroStent 120 cm delivery catheter, as well as MicroStent devices in five lengths and five diameters to meet individual patient needs. MicroStent is a vascular stent specifically designed to reduce below-the-knee amputations caused by critical limb ischemia resulting from peripheral artery disease. [Micro Medical Solutions (PR Newswire Association LLC.)] Press Release | |
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POLICY NEWSWellcome and Gates Join Bold European Open-Access Plan Two of the world’s largest biomedical research funders have backed a plan to make all papers resulting from work they fund open access on publication by 2020. The London-based Wellcome Trust and the Bill and Melinda Gates Foundation in Seattle, Washington, announced they were both endorsing ‘Plan S’, adding their weight to an initiative already backed by 13 research funders across Europe since its launch in September. [Nature News] Editorial Record Number of Monkeys Being Used in U.S. Research The number of monkeys used in U.S. biomedical research reached an all-time high last year, according to data released in late September by the United States Department of Agriculture. [ScienceInsider] Editorial NSF Suspends Program Allowing Graduate Fellows to Study Abroad The U.S. National Science Foundation (NSF) has halted a program that each year allows hundreds of the nation’s best graduate students to work with experts in another country. And the agency isn’t saying why—or whether the program will resume. [ScienceInsider] Editorial
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EVENTSNEW EMBL: Proteomics in Cell Biology and Disease Mechanisms Visit our events page to see a complete list of events in the community.
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JOB OPPORTUNITIESNEW Postdoctoral Positions – Cell/Developmental/Vascular Biology (Uppsala University) Postdoctoral Fellow – Endothelial Cell Research (University of Missouri) Postdoctoral Research Associate – Innate Immunity and Inflammation (University of Illinois) Postdoctoral Fellow – Vascular Research (Johns Hopkins University School of Medicine) Postdoctoral Fellow – Vascular Development (University of Colorado Denver) Group Leader – Vascular Research (Helmholtz Zentrum München) Postdoctoral Fellow – Vascular and/or Lung Biology (Yale University) Postdoctoral Scientists – Vascular Diseases (Oklahoma Medical Research Foundation) Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.
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