Enhanced SMAD1 Signaling Contributes to Impairments of Early Development in CFC-iPSCs Scientists show that both SMAD1 and ERK signaling pathways may contribute to the developmental defects in cardio-facio-cutaneous (CFC) syndrome. Induced pluripotent stem cells derived from dermal fibroblasts of a CFC syndrome patient (CFC-iPSCs) revealed early developmental defects in embryoid body development, β-catenin localization, and neuronal differentiation. [Stem Cells] Abstract Novel Codon-Optimized Mini-Intronic Plasmid for Efficient, Inexpensive, and Xeno-Free Induction of Pluripotency Researchers developed a single plasmid reprogramming system which carries codon-optimized sequences of the canonical reprogramming factors (Oct4, Klf4, Sox2, and c-Myc) and short hairpin RNA against p53. They derived human and mouse induced pluripotent stem cell lines from fibroblasts by performing a single transfection. [Sci Rep] Full Article Micropattern Size-Dependent Endothelial Differentiation from a Human Induced Pluripotent Stem Cell Line Investigators examined the hypothesis that the diameter of micropatterned islands influences endothelial differentiation from human induced pluripotent stem cells. [J Tissue Eng Regen Med] Abstract Cdx2 Efficiently Induces Trophoblast Stem-Like Cells in Naïve, but Not Primed, Pluripotent Stem Cells Researchers compared the capacity of mouse embryonic stem cells, induced pluripotent stem cells, embryonal carcinoma cells, and epiblast stem cells to form trophoblasts. [Stem Cells Dev] Abstract β2-Adrenoreceptor-Mediated Proliferation Inhibition of Embryonic Pluripotent Stem Cells Investigators identified a β2-adrenoreceptor/AC/cAMP/PKA signaling pathway in embryonic pluripotent stem cells and found that the pathway stimulation inhibited proliferation and cell cycle progression involving modulating the stem cell growth and cycle regulatory machinery. [J Cell Physiol] Abstract Human iPS Cell-Derived Insulin Producing Cells Form Vascularized Organoids under the Kidney Capsules of Diabetic Mice Using a series of pancreatic growth factors, scientists successfully generated induced pluripotent stem (iPS) cells derived insulin producing cells. To investigate the capability of these cells to secrete insulin in vivo, the differentiated cells were transplanted under the kidney capsules of diabetic immunodeficient mice. [PLoS One] Full Article | Press Release Early Intervention for Spinal Cord Injury with Human Induced Pluripotent Stem Cells Oligodendrocyte Progenitors Scientists demonstrate for the first time that oligodendrocyte progenitors can be derived from induced pluripotent stem cells generated using either an episomal, non-integrating plasmid approach or standard integrating retroviruses that survive and differentiate into mature oligodendrocytes after early transplantation into the injured spinal cord. [PLoS One] Full Article Stable X Chromosome Reactivation in Female Human Induced Pluripotent Stem Cells To investigate whether reprogramming of female human fibroblasts into induced pluripotent stem cells leads to reactivation of the inactive X chromosome, researchers generated induced pluripotent stem cell lines from fibroblasts heterozygous for large X-chromosomal deletions. [Stem Cell Reports] Full Article | Graphical Abstract |