Elucidating Molecular Phenotypes Caused by the SORL1 Alzheimer’s Disease Genetic Risk Factor Using Human Induced Pluripotent Stem Cells Scientists used human induced pluripotent stem cells to examine genetic variation in the SORL1 gene and possible contributions to sporadic Alzheimer’s disease-related phenotypes in human neurons. [Cell Stem Cell] Abstract | Graphical Abstract | Press Release Extensive MicroRNA-Mediated Crosstalk between lncRNAs and mRNAs in Mouse Embryonic Stem Cells To gain insight into long noncoding RNA (lncRNA)-mRNA miRNA-mediated crosstalk, investigators analyzed transcriptome-wide changes induced by the targeted knockdown of over 100 lncRNA transcripts in mouse embryonic stem cells. [Genome Res] Abstract In Vitro Generation of Human Pluripotent Stem Cell Derived Lung Organoids Researchers report the step-wise differentiation of human pluripotent stem cells into lung organoids. By manipulating developmental signaling pathways hPSCs generate ventral-anterior foregut spheroids, which are then expanded into human lung organoids. [eLife] Full Article | Press Release Modeling and Studying Mechanism of Dilated Cardiomyopathy Using Induced Pluripotent Stem Cells Derived from Duchenne Muscular Dystrophy (DMD) Patients To study the molecular mechanisms underlying dilated cardiomyopathy in DMD heart, researchers generated cardiomyocytes from DMD and healthy control induced pluripotent stem cells. [Dis Model Mech] Abstract Factor Induced Reprogramming and Zinc Finger Nuclease Aided Gene Targeting Cause Different Genome Instability in β-Thalassemia iPSCs To examine the mutation status of the edited induced pluripotent stem cell (iPSC) genome and trace the origin of possible mutations at different steps, scientists generated virus-free iPSCs from amniotic cells carrying homozygous point mutations in β-hemoglobin gene (HBB) that cause severe β-thalassemia, corrected the mutations in both HBB alleles by ZFN-aided gene targeting, and obtained the final HBB gene corrected iPSCs by excising the exogenous drug resistant gene with Cre recombinase. [J Biol Chem] Abstract | Full Article RELB Alters Proliferation of Human Pluripotent Stem Cells via IMP3- and LIN28-Mediated Modulation of the Expression of IGF2 and Other Cell-Cycle Regulators The authors showed that RELB enhances the proliferation of hESCs and human induced pluripotent stem cells without affecting their pluripotency. They demonstrated that RELB does this by interacting with two RNA-binding proteins LIN28A and IMP3 (IGF-2 mRNA-binding protein 3); further, these interactions control mRNA levels and protein expression of IGF-2 and key cell-cycle genes. [Stem Cells Dev] Abstract Genetic Heterogeneity of Induced Pluripotent Stem Cells: Results from 24 Clones Derived from a Single C57BL/6 Mouse To assess the degree of genetic heterogeneity, and to determine whether some cells are more genetically “fit” for reprogramming, scientists performed exome sequencing on 24 mouse induced pluripotent stem cell clones derived from skin fibroblasts obtained from two different sites of the same eight-week-old C57BL/6J male mouse. [PLoS One] Full Article Reprogramming of HUVECs into Induced Pluripotent Stem Cells (HiPSCs), Generation and Characterization of hiPSC-Derived Neurons and Astrocytes With the objective of establishing reliable tools to study neurodegenerative diseases, researchers reprogrammed human umbilical vein endothelial cells (HUVECs) into iPSCs. Using a novel and direct approach, HiPSCs were differentiated into cells of central nervous system lineage, including neuronal, astrocyte and glial cells, with high efficiency. [PLoS One] Full Article |