A Short G1 Phase Imposes Constitutive Replication Stress and Fork Remodeling in Mouse Embryonic Stem Cells Researchers showed in cultured embryonic stem cells and mouse embryos that H2AX phosphorylation is dependent on ataxia telangiectasia and Rad3 related and is associated with chromatin loading of the ssDNA-binding proteins RPA and RAD51. [Nat Commun] Full Article Initiation of Stem Cell Differentiation Involves Cell Cycle-Dependent Regulation of Developmental Genes by Cyclin D Investigators demonstrated that cell cycle regulators Cyclin D1–3 control cell fate decisions in human pluripotent stem cells by recruiting transcriptional corepressors and coactivator complexes onto neuroectoderm, mesoderm, and endoderm genes. [Genes Dev] Abstract Aurkb/PP1-Mediated Resetting of Oct4 during the Cell Cycle Determines the Identity of Embryonic Stem Cells Investigators demonstrated that the regulation of Oct4 by aurora kinase b (Aurkb)/protein phosphatase 1 (PP1) during the cell cycle is important for resetting Oct4 to pluripotency and cell cycle genes in determining the identity of embryonic stem cells. [eLife] Abstract | Download Full Article NRF2 Orchestrates the Metabolic Shift during Induced Pluripotent Stem Cell Reprogramming Scientists developed a lentiviral reporter system to assay longitudinal changes in cell signaling and transcription factor activity in living cells throughout induced pluripotent stem cells reprogramming of human dermal fibroblasts. [Cell Rep] Full Article | Graphical Abstract Enrichment of G2/M Cell Cycle Phase in Human Pluripotent Stem Cells Enhances HDR-Mediated Gene Repair with Customizable Endonucleases Investigators observed improved efficiency using ZFNs, TALENs, two CRISPR/Cas9, and CRISPR/Cas9 nickase to target five genes in three human pluripotent stem cells lines. [Sci Rep] Full Article TRPV-1-Mediated Elimination of Residual iPS Cells in Bioengineered Cardiac Cell Sheet Tissues Researchers showed that TRPV-1 activation through transient culture at 42°C in combination with agonists is a simple and useful strategy to eliminate induced pluripotent stem cells from bioengineered cardiac cell sheet tissues. [Sci Rep] Full Article SETD7 Regulates the Differentiation of Human Embryonic Stem Cells The authors identified the methyltransferase SETD7 as highly induced during the differentiation of human embryonic stem cells and differentially expressed between induced pluripotent cells and somatic cells. [PLoS One] Full Article Mucin-Inspired Thermoresponsive Synthetic Hydrogels Induce Stasis in Human Pluripotent Stem Cells and Human Embryos Researchers report cellular stasis for both human pluripotent stem cells colonies and preimplantation embryos immersed in a wholly synthetic thermoresponsive gel comprising poly(glycerol monomethacrylate)-poly(2-hydroxypropyl methacrylate) diblock copolymer worms. [ACS Cent Sci] Full Article | Press Release |