An Epigenetic Switch Regulates De Novo DNA Methylation at a Subset of Pluripotency Gene Enhancers during Embryonic Stem Cell Differentiation Scientists investigated the effect of the Lsd1-Mi2/NuRD complex on the dynamic regulatory switch that induces the local interaction of histone tails with the Dnmt3 ATRX-DNMT3-DNMT3L domain, thus promoting DNA methylation at the enhancers of a subset of pluripotency genes. [Nucleic Acids Res] Full Article Tet Enzymes Regulate Telomere Maintenance and Chromosomal Stability of Mouse ESCs The authors showed that mouse embryonic stem cells (ESCs) depleted of ten-eleven translocation (Tet) 1 and/or Tet2 by RNAi exhibit short telomeres and chromosomal instability, concomitant with reduced telomere recombination. [Cell Rep] Full Article | Graphical Abstract Genome Therapy of Myotonic Dystrophy Type 1 iPS Cells for Development of Autologous Stem Cell Therapy Investigators developed genome therapy in human myotonic dystrophy type 1 induced pluripotent stem (iPS) cells to eliminate mutant transcripts and reverse the phenotypes for developing autologous stem cell therapy. [Mol Ther] Abstract Rapid Induction of Cerebral Organoids From Human Induced Pluripotent Stem Cells Using a Chemically Defined Hydrogel and Defined Cell Culture Medium Researchers described a robust and simple method for generating cerebral organoids from human pluripotent stem cells by using a chemically defined hydrogel material and chemically defined culture medium. [Stem Cells Transl Med] Abstract Generation and Gene Expression Profiling of 48 Transcription-Factor-Inducible Mouse Embryonic Stem Cell Lines Previously, the authors generated and analyzed 137 transcription factor (TF)-inducible mouse embryonic stem cells (ESCs) lines. As an extension of this “NIA Mouse ESC Bank,” they generated and characterized 48 additional mouse ESC lines, in which single TFs in each line could be induced in a doxycycline-controllable manner. [Sci Rep] Full Article Derivation of Porcine Embryonic Stem-Like Cells from In Vitro-Produced Blastocyst-Stage Embryos Investigators described the derivation of porcine embryonic stem-like cells by seeding the isolated inner cell mass from in vitro-produced porcine blastocyst into α-MEM with basic fibroblast growth factor. [Sci Rep] Full Article Generation and Characterization of Integration-Free Induced Pluripotent Stem Cells from Patients with Autoimmune Disease Scientists demonstrated the successful generation of integration-free induced pluripotent stem cells from patients with ankylosing spondylitis, Sjögren’s syndrome and systemic lupus erythematosus. [Exp Mol Med] Full Article Multiple Sclerosis Patient-Specific Primary Neurons Differentiated from Urinary Renal Epithelial Cells via Induced Pluripotent Stem Cells Renal proximal tubule epithelial cells were isolated from multiple sclerosis patients’ urine and transfected with pluripotency-inducing episomal factors. Subsequent induced pluripotent stem cells were formed into embryoid bodies selective for ectodermal lineage, resulting in neural tube-like rosettes and eventually neural progenitor cells. [PLoS One] Full Article |